Article
Chemistry, Analytical
Yangping Li, Yuxiang Li, Hongjing Chen, Wenting Zhang, Yang Yan
Summary: This study developed a universal method for detecting acrylamide (AA) in various foods and analyzed its distribution in 437 food samples collected from Southeast China. The method was simple, rapid, and widely applicable, with good recovery rate and precision. AA was detected in all types of food, with varying levels ranging from LOQ to 6020 μg/kg. Significant differences in AA levels among different foods were observed. The developed method provides a useful tool for monitoring AA levels in food.
ANALYTICAL METHODS
(2023)
Article
Endocrinology & Metabolism
Zheng-yu Yang, Yi-ying Wu, Yi Zhou, Yun-qi Yang, Jia-hui Zhang, Tao He, Sha Liu
Summary: NITyr reduced lipid accumulation by improving lipid and glucose metabolism involving CB1 and CB2 receptors, without affecting appetite-related indexes and other organ functions.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rodrigo Zamith Cunha, Alberto Semprini, Giulia Salamanca, Francesca Gobbo, Maria Morini, Kirstie J. Pickles, Veronica Roberts, Roberto Chiocchetti
Summary: This study investigated the expression of cannabinoid receptors and related receptors in the trigeminal ganglia of horses and found that they are expressed in sensory neurons. The study suggests further investigation of these receptors in equine trigeminal neuronal disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Hongying Gao, Yi Lin, Jie Wei, Yike Zhang, Hong Pan, Miaomiao Ren, Jin Li, Liuying Huang, Xi Zhang, Qingyu Huang, Heqing Shen
Summary: This study developed a method to quantify the presence of NPs in animal tissues, selectively enriching PS-NP through a CSE protocol for analysis. The method was verified in experiments and provides a reference for assessing human exposure to NPs and associated health risks.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Analytical
Hehe Tian, Haichao Wen, Xinquan Yang, Siying Li, Jingming Li
Summary: This study utilized two different chromatography methods to analyze the volatile organic compounds in feces and urine of AD model mice treated with anthocyanins, revealing differences in VOCs content between the AD model group and the anthocyanin control group, which may provide insights into the mechanism of action of anthocyanins on AD.
MICROCHEMICAL JOURNAL
(2021)
Article
Food Science & Technology
Safae Chafi, Evaristo Ballesteros
Summary: The presence of chemical contaminants in food can seriously compromise food safety, creating a need for efficient analytical methods to detect these contaminants. In this study, a simple, sensitive, and fast analytical method was developed to determine thirteen natural and synthetic hormones in meat and fish products. The method showed good accuracy and sensitivity, and was able to remove potential interferences from the samples.
Article
Food Science & Technology
Marzieh Kamankesh, Fatemeh Barzegar, Nabi Shariatifar, Abdorreza Mohammadi
Summary: In this study, a combination of HF-EME and DLLME followed by GC/MS was applied to analyze the biogenic amines in non-alcoholic beers. The method showed advantages in improving selectivity and enrichment factor, which could help beverage industries to monitor and control the concentration of biogenic amines in beers.
Article
Energy & Fuels
Shiqi Wang, Hailong Zuo, Chun-ning Gao, Jing-hua Wang, Congcong Li, Shuoliang Wang
Summary: In this study, we used advanced mass spectrometry techniques to analyze crude oil compounds and proposed a new analytical approach. The method involved three-dimensional visualization of mass spectrometry data, multivariate statistical analyses, and a combination of multivariate statistical and conventional analytical methods. The results identified specific compounds as differential markers for distinguishing between different crude oil samples. The study also discussed the potential application of this method in petroleum-contaminated groundwater.
Article
Nutrition & Dietetics
Dadi Xie, Yanbo Zhang, Yujin Guo, Xianzhong Xue, Shiyuan Zhao, Chunmei Geng, Yuanyuan Li, Rui Yang, Yizhang Gan, Hanbing Li, Zhongfa Ren, Pei Jiang
Summary: The objective of this research was to observe and evaluate the impact of dietary components on metabolomic profiles in primary tissues of mice. The levels of metabolites in mice were studied and analyzed using gas chromatography-mass spectrometry. The results showed significant differences in metabolites related to energy, amino acids, and lipid metabolism.
FRONTIERS IN NUTRITION
(2023)
Article
Multidisciplinary Sciences
Liying Zhang, Charlotte Simonsen, Lucie Zimova, Kaituo Wang, Lavanya Moparthi, Rachelle Gaudet, Maria Ekoff, Gunnar Nilsson, Ute A. Hellmich, Viktorie Vlachova, Pontus Gourdon, Peter M. Zygmunt
Summary: This study uses cryo-EM and electrophysiology to identify a cannabinoid binding site in TRPV2 that is distinct from cannabidiol, which could potentially be a drug target for the treatment of inflammation and immune-mediated diseases.
NATURE COMMUNICATIONS
(2022)
Article
Environmental Sciences
Samia Mokh, Rania Nassar, Atika Berry, Mohammad El Khatib, Samah Doumiati, Mariam Taha, Raed Ezzeddine, Mohamad Al Iskandarani
Summary: The study utilized a solid-phase extraction method combined with liquid and gas chromatography-mass spectrometry to determine 18 organic UV filters in water samples, successfully detecting 8 target compounds. The method showed good recovery rates and acceptable repeatability and reproducibility, providing data support for future evaluation of UV filter residues in Lebanese swimming pools and their impact on the ecosystem.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biochemical Research Methods
Lei He, Yuanji Gao, Lijuan Zhao
Summary: The combination of bubbling extraction and gas chromatography-mass spectrometry enables efficient qualitative and quantitative analysis of volatiles in beer, with high extraction efficiency, short analysis time, and rational recovery rates. The method is suitable for a wide concentration range of components and has the potential to be a routine analysis tool for alcoholic beverages.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Environmental Sciences
Ming Yang, Shili Tian, Qingyang Liu, Zheng Yang, Yifan Yang, Peng Shao, Yanju Liu
Summary: This method established a quantitative method for the simultaneous determination of priority and non-priority PAHs in plant leaves using GC-MS. The method is highly sensitive, with good linearity and low detection limits, and demonstrated high spiked recovery rates.
Article
Chemistry, Analytical
Mohammed Moufid, Michael Hofmann, Nezha El Bari, Carlo Tiebe, Matthias Bartholmai, Benachir Bouchikhi
Summary: The presence of wastewater and air pollution poses a significant risk to citizens and the country's image. Electronic nose and Voltammetric Electronic tongue are utilized to discriminate between polluted and clean environmental samples, with chemometric tools for data processing. These systems are proven to be valuable for analyzing polluted matrices.
Article
Chemistry, Multidisciplinary
Fangfang Zhu, Honglong Liu, Wenqing Zhang, Chuanyuan Du, Hao Zhu, Xiaodi Du, Xiaosong Hu, Yawei Lin
Summary: A novel BSMAD derivative was synthesized for the quantitative analysis of BAs in alcoholic beverages, and a HPLC method with low detection limit and excellent repeatability was successfully established. The method was applied for BA determination in various types of alcoholic beverages with satisfactory recoveries, providing a reliable and sensitive approach for future studies.
Article
Biotechnology & Applied Microbiology
Shanmukh S. Salimath, Trevor B. Romsdahl, Anji Reddy Konda, Wei Zhang, Edgar B. Cahoon, Michael K. Dowd, Thomas C. Wedegaertner, Kater D. Hake, Kent D. Chapman
Summary: Upland cotton is an economically important crop globally cultivated for various purposes. Transgenic cotton lines expressing a barley gene showed increased accumulation of vitamin E without negative impacts on plant performance; in fact, it enhanced antioxidant activity and oxidative stability of cottonseed oil, potentially providing new opportunities for improved cottonseed co-products.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Review
Plant Sciences
Ashley E. Cannon, Kent D. Chapman
Summary: N-Acylethanolamine (NAE) signaling is important in both vertebrates and plants, where G proteins play a crucial role in transmitting the signal. While similarities exist between plants and animals in NAE signaling, there are also distinct differences, highlighting the complexity of this fundamental lipid signaling pathway in eukaryotes.
TRENDS IN PLANT SCIENCE
(2021)
Article
Ophthalmology
Stephanie L. Grillo, Justin D. Etzel, Sarah R. Weber, Cassandra Ondeck, Weiwei Wang, Yuanjun Zhao, Alistair J. Barber, Jeffrey M. Sundstrom
Summary: The study found an association between Fibulin-3 (Fib3) and drusen as well as extracellular vesicles (EVs), showing different distribution patterns in age-related macular degeneration (AMD).
EXPERIMENTAL EYE RESEARCH
(2021)
Review
Cell Biology
Salvatore L. Stella, Jasmine S. Geathers, Sarah R. Weber, Michael A. Grillo, Alistair J. Barber, Jeffrey M. Sundstrom, Stephanie L. Grillo
Summary: Neurodegenerative retinal diseases lead to gradual loss of retinal neurons, requiring neuroprotective or regenerative strategies for treatment. Zebrafish, due to their similar eye structure and development to mammals, are commonly used as a model for studying these diseases.
Article
Biochemistry & Molecular Biology
Trevor B. Romsdahl, Shrikaar Kambhampati, Somnath Koley, Umesh P. Yadav, Ana Paula Alonso, Doug K. Allen, Kent D. Chapman
Summary: The combination of C-13-isotopic labeling and mass spectrometry imaging offers a method to analyze metabolic flux, but presents technical challenges. Results from studying embryos of oilseed crops show greater C-13-isotopic labeling in cotyledons, with isotopic enrichment in PC molecular species suggesting different flux patterns related to fatty acid pathways.
Review
Biochemistry & Molecular Biology
Byung-Ho Kang, Charles T. Anderson, Shin-ichi Arimura, Emmanuelle Bayer, Magdalena Bezanilla, Miguel A. Botella, Federica Brandizzi, Tessa M. Burch-Smith, Kent D. Chapman, Kai Duenser, Yangnan Gu, Yvon Jaillais, Helmut Kirchhoff, Marisa S. Otegui, Abel Rosado, Yu Tang, Juergen Kleine-Vehn, Pengwei Wang, Bethany Karlin Zolman
Summary: The article summarizes a modern view of plant cell structures and unresolved mysteries, discussing the complexities of internal structures and the shapes revealed by new microscopy techniques. Future research will continue to focus on the combination of imaging modalities with functional studies.
Article
Biochemistry & Molecular Biology
Stepan Fenyk, Helen K. Woodfield, Trevor B. Romsdahl, Emma J. Wallington, Ruth E. Bates, David A. Fell, Kent D. Chapman, Tony Fawcett, John L. Harwood
Summary: This study investigated the effects of expressing PDAT1 in oil seeds on lipid metabolism, showing that PDAT1 overexpression results in a slight decrease in seed oil content and altered fatty acid composition. This has important implications for regulating oil accumulation in crops.
BIOCHEMICAL JOURNAL
(2022)
Article
Plant Sciences
Yingqi Cai, Zhiyang Zhai, Jantana Blanford, Hui Liu, Hai Shi, Jorg Schwender, Changcheng Xu, John Shanklin
Summary: The study demonstrated that PAP2 can stimulate a cycle of fatty acid synthesis and degradation, mitigating negative effects of high TAG accumulation on plant growth. By combining PAP2 with TAG-promoting factors, carbon assimilation, fatty acid synthesis, and allocation to TAGs can be enhanced for optimized plant growth and storage lipid accumulation.
Review
Plant Sciences
Patricia Scholz, Kent D. Chapman, Robert T. Mullen, Till Ischebeck
Summary: In recent years, the number of contact sites between subcellular compartments in eukaryotic cells has increased, highlighting their essential role in cellular organization and functioning. This article discusses various contact sites involving plant lipid droplets, including their role in biogenesis and degradation, as well as newly discovered connections with unknown physiological functions.
Article
Plant Sciences
Omar Arias-Gaguancela, Bikash Adhikari, Mina Aziz, Kent D. Chapman
Summary: The study shows that PDP-EA can enhance FAAH enzyme activity to promote NAE hydrolysis in upland cotton seedlings, resulting in enhanced growth. This research expands the role of NAE metabolism in seedling development to plant species beyond the model Arabidopsis.
Article
Plant Sciences
Zhiyang Zhai, Jantana K. Blanford, Yingqi Cai, Jing Sun, Hui Liu, Hai Shi, Jorg Schwender, John Shanklin
Summary: CDK8 plays a crucial role in lipid synthesis regulation. It promotes lipid synthesis by activating WRINKLED1 through its interaction with GEMINIVIRUS REP INTERACTING KINASE1. However, KIN10 can degrade CDK8 and inhibit lipid synthesis.
Article
Plant Sciences
Omar Arias-Gaguancela, Mina Aziz, Kent D. Chapman
Summary: In cotton plants, both FAAH and LOX enzymes are involved in the metabolism and regulation of 9-NAE-HOD, which negatively affects seedling growth. Silencing FAAH genes leads to elevated levels of 9-NAE-HOD and inhibited seedling growth, while silencing LOX genes reverses this effect. These findings expand our understanding of plant growth modulation by NAE oxylipins.
Review
Plant Sciences
Ljudmilla Borisjuk, Patrick Horn, Kent Chapman, Peter M. Jakob, Andre Guendel, Hardy Rolletschek
Summary: Imaging has long supported our understanding of plants' inner life, development, and response to the environment. Novel technologies, relying on NMR, MS, or IR spectroscopy, are making significant contributions to visualizing plant metabolism. This review provides an overview of current imaging methods, discusses their advantages and limitations, presents examples of their application, and suggests their potential for experimental practice, encouraging progress in plant science.
Review
Plant Sciences
Patrick J. Horn, Kent D. Chapman
Summary: Mass spectrometry imaging (MSI) is a valuable analytical technique used to investigate the spatial distribution of molecules within biological systems. In the realm of plant science, MSI has been increasingly employed to explore the distribution patterns of diverse metabolites and phytochemicals, facilitating the understanding of their metabolic pathways and functional roles.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Lani Archer, Hossain A. Mondal, Sumita Behera, Moon Twayana, Monika Patel, Joe Louis, Vamsi J. Nalam, Jantana Keereetaweep, Zulkarnain Chowdhury, Jyoti Shah
Summary: Interaction between MYZUS PERSICAE-INDUCED LIPASE 1 function in the phloem, and 12-oxo-phytodienoic acid (OPDA) and CYCLOPHILIN 20-3 is involved in limiting green peach aphid infestation on Arabidopsis thaliana.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)