4.2 Article

Isolation and characterization of twenty-four polymorphic microsatellite loci for the topical seagrass, Thalassia hemprichii

Journal

CONSERVATION GENETICS RESOURCES
Volume 5, Issue 4, Pages 939-941

Publisher

SPRINGER
DOI: 10.1007/s12686-013-9937-1

Keywords

Connectivity; Gene flow; Indonesia; Conservation

Funding

  1. TOTAL Foundation
  2. University of Hawai'i Graduate Student Organization
  3. University of Hawai'i Arts and Sciences Student Research Award
  4. University of Hawai'i Edmondson Grant
  5. Sigma XI
  6. Explorers Club
  7. Rufford Small Grants Foundation
  8. University of Hawai'i NSF-EPSCoR program [EPS-0903833]

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Twenty-four polymorphic microsatellite loci were developed for the tropical seagrass, Thalassia hemprichii. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Pacific Biosciences SMRT technology. The 24 loci were characterized in 19 individuals from the Tukang Besi Islands, South East Sulawesi, Indonesia. Observed levels of heterozygosity ranged from 0.26 to 0.95 with a mean of 4.7 alleles per locus. No pairs of loci showed evidence of significant linkage disequilibrium and one locus significantly deviated from Hardy-Weinberg expectations.

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