4.2 Article

Characterization of nine microsatellite loci from the endangered southern brown bandicoot (Isoodon obesulus) using 454 pyrosequencing

Journal

CONSERVATION GENETICS RESOURCES
Volume 5, Issue 1, Pages 105-107

Publisher

SPRINGER
DOI: 10.1007/s12686-012-9743-1

Keywords

Isoodon obesulus; Endangered marsupial; Microsatellites; Partial 454 shotgun pyrosequencing

Funding

  1. Australian Research Council [LP0668987]
  2. Native Vegetation Council
  3. Wildlife Conservation Fund
  4. Nature Foundation SA
  5. Australian Research Council [LP0668987] Funding Source: Australian Research Council

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Nine polymorphic microsatellite markers were developed for a nationally endangered marsupial, the southern brown bandicoot (Isoodon obesulus) using a next generation sequencing approach. The nine markers were genotyped in 59 individuals from two distinct locations (the Mt Burr Range and the Mt Lofty Ranges) in South Australia. All loci showed Hardy-Weinberg equilibrium with the exception of one locus in the Mt Lofty population, possibly because of null alleles. No evidence of linkage disequilibrium was detected. These markers will provide valuable resources for future projects on the conservation genetics of southern brown bandicoots in Australia.

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