☆ 4.2 Article

Mogurnda adspersa microsatellite markers: multiplexing and multi-tailed primer tagging

CONSERVATION GENETICS RESOURCES (2009)

Journal

CONSERVATION GENETICS RESOURCES

Volume 1, Issue 1, Pages 411-414

Publisher

SPRINGER

DOI: 10.1007/s12686-009-9095-7

Keywords

Freshwater fish; Purple-spotted gudgeon; Eleotridae; M13 tailing; Multiplex PCR

Categories

Biodiversity Conservation Genetics & Heredity

Funding

DERM

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Abstract

A set of twelve microsatellite DNA loci were developed for the threatened Australian freshwater fish Mogurnda adspersa (Eleotridae). Primers were tailed with one of four 20-mer oligonucleotides for use in four-colour fluorescent detection and optimised for multiplex PCR. The loci were used to genotype individuals from two populations in the Pioneer River catchment of central Queensland, eastern Australia. Number of alleles per locus ranged from 2 to 33 and per locus heterozygosity ranged from 0.06 to 0.81. Successful cross-species amplification of all loci was achieved in the congener M. mogurnda. These markers will be used to estimate effective population size and to examine the relationship between flow regime and population demographic parameters.

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