Journal
LIGHT-SCIENCE & APPLICATIONS
Volume 4, Issue -, Pages -Publisher
CHINESE ACAD SCIENCES, CHANGCHUN INST OPTICS FINE MECHANICS AND PHYSICS
DOI: 10.1038/lsa.2015.34
Keywords
computational imaging; lensfree microscopy; on-chip microscopy; synthetic aperture
Categories
Funding
- Presidential Early Career Award for Scientists and Engineers (PECASE)
- Army Research Office (ARO) [W911NF-13-1-0419, W911NF-13-1-0197]
- ARO Life Sciences Division
- ARO Young Investigator Award
- National Science Foundation (NSF) CAREER Award
- NSF CBET Division Biophotonics Program
- NSF Emerging Frontiers in Research and Innovation (EFRI) Award
- NSF EAGER Award
- Office of Naval Research (ONR)
- Howard Hughes Medical Institute (HHMI)
- National Institutes of Health (NIH) Director's New Innovator Award from the Office of the Director, National Institutes of Health [DP2OD006427]
- National Science Foundation under the American Recovery and Reinvestment Act of 2009 (ARRA) [0963183]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0954482] Funding Source: National Science Foundation
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Wide field-of-view (FOV) and high-resolution imaging requires microscopy modalities to have large space-bandwidth products. Lensfree on-chip microscopy decouples resolution from FOV and can achieve a space-bandwidth product greater than one billion under unit magnification using state-of-the-art opto-electronic sensor chips and pixel super-resolution techniques. However, using vertical illumination, the effective numerical aperture (NA) that can be achieved with an on-chip microscope is limited by a poor signal-to-noise ratio (SNR) at high spatial frequencies and imaging artifacts that arise as a result of the relatively narrow acceptance angles of the sensor's pixels. Here, we report, for the first time, a synthetic aperture-based on-chip microscope in which the illumination angle is scanned across the surface of a dome to increase the effective NA of the reconstructed lensfree image to 1.4, achieving e.g., similar to 250-nm resolution at 700-nm wavelength under unit magnification. This synthetic aperture approach not only represents the largest NA achieved to date using an on-chip microscope but also enables color imaging of connected tissue samples, such as pathology slides, by achieving robust phase recovery without the need for multi-height scanning or any prior information about the sample. To validate the effectiveness of this synthetic aperture-based, partially coherent, holographic on-chip microscope, we have successfully imaged color-stained cancer tissue slides as well as unstained Papanicolaou smears across a very large FOV of 20.5 mm(2). This compact on-chip microscope based on a synthetic aperture approach could be useful for various applications in medicine, physical sciences and engineering that demand high-resolution wide-field imaging.
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