Review
Cell Biology
Joseph O. Magliozzi, James B. Moseley
Summary: Cell polarity signaling pathways play a direct role in regulating the contractile actomyosin ring (CAR) during cytokinesis, contributing to both local organization of CAR proteins and structures, as well as global organization of the division plane within a cell. Recent phosphoproteomics studies have identified new connections between cell polarity kinases and CAR proteins, pointing to conserved processes across different organisms. Opportunities for future research exist to further explore the conservation and divergence in the cell division process among organisms.
Article
Biochemistry & Molecular Biology
Mathieu C. Husser, Imge Ozugergin, Tiziana Resta, Vincent J. J. Martin, Alisa J. Piekny
Summary: Cytokinesis is a crucial process that requires the assembly and ingression of an actomyosin ring to physically separate daughter cells. By studying the localization of key molecules during cytokinesis in live human cells, differences in cytokinetic diversity were observed among different cell types.
Article
Biochemistry & Molecular Biology
Lina Liu, Jiaju Xu, Ruiying Na, Renpeng Du, Wenxiang Ping, Jingping Ge, Dan Zhao
Summary: In this study, the extraction, purification, and characterization of an exopolysaccharide (EPS) from Saccharomyces cerevisiae Y3 were investigated. The EPS was found to be a hetero-polysaccharide composed of various sugars and exhibited good hydrophilicity, thermal stability, and antioxidation ability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Microbiology
Cesar Roncero, Ruben Celador, Noelia Sanchez, Patricia Garcia, Yolanda Sanchez
Summary: Cytokinesis divides a mother cell into two daughter cells using a contractile actomyosin ring (CAR), contributing to genome integrity. While differences exist in the final steps of cytokinesis in animal cells and yeast, the signalling pathways play a critical role in cell wall remodeling during cytokinesis in yeast.
Article
Cell Biology
Zachary McDargh, Tianyi Zhu, Hongkang Zhu, Ben O'Shaughnessy
Summary: This study used molecular simulations to investigate the role of cofilin in the contractile ring during cell division. The simulations revealed that the absence of cofilin led to an increase in ring tension, resulting in bridging instabilities. This research identified the critical role of cofilin in actomyosin turnover and its importance in protecting the contractile ring during cytokinesis.
JOURNAL OF CELL SCIENCE
(2023)
Article
Chemistry, Analytical
Yu-ki Tanaka, Shunsuke Shimazaki, Yasunori Fukumoto, Yasumitsu Ogra
Summary: A rapid and precise quantification method for a histidine-tagged recombinant protein produced in Escherichia coli (E. coli) has been developed using single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS). This method allows for the determination of protein quantity based on the signal of nickel or cobalt ions bound to the His-tag, without the need for extraction and purification processes.
ANALYTICAL CHEMISTRY
(2022)
Review
Immunology
Alexander A. Morano, Jeffrey D. Dvorin
Summary: The actomyosin contractile ring is a conserved feature in eukaryotic cytokinesis, but the structure and force generation mechanism of the basal complex in Apicomplexans, which lack myosin II homolog, remain poorly understood. By comparing with well-studied cytokinetic mechanisms in other organisms, new research directions and possible answers are suggested to explore the diversity and divergence of Apicomplexans.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Anthony M. Rossi, Kathleen L. Gould, K. Adam Bohnert
Summary: This study explored the role of the cytokinetic ring component Fic1 in septum formation. It was found that Fic1 promotes septum formation by interacting with F-BAR proteins Cdc15 and Imp2, as well as with Cyk3. The findings indicate differences in the mechanisms of cell division in different yeasts.
Article
Cell Biology
Sandy Ibanes, Fatima El-Alaoui, Josephine Lai-Kee-Him, Chantal Cazevieille, Francois Hoh, Sebastien Lyonnais, Patrick Bron, Luca Cipelletti, Laura Picas, Simonetta Piatti
Summary: This study reveals that Syp1 is able to align and pack septin filaments laterally, aiding in the assembly of septin collar. It also emphasizes the conserved protein functions and the functional diversity of unstructured protein domains.
Article
Engineering, Chemical
Beatriz B. Cardoso, Jean-Michel Fernandes, Ana C. Pinheiro, Adelaide Braga, Sara C. Silverio, Ligia R. Rodrigues
Summary: The aim of this study was to establish a food-grade purification strategy for the production and purification of Epilactose, a functional sugar. Epilactose was produced by enzymatic epimerization and unreacted lactose was removed using a suitable 8-galactosidase enzyme. Monosaccharides generated during the reaction were eliminated through microbial treatment or activated charcoal. The resulting two-step process achieved an 87% purity and 76.4% yield of Epilactose. Additionally, the resistance of Epilactose to gastrointestinal conditions was evaluated using in vitro simulation, demonstrating its potential as a prebiotic.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Cell Biology
MariaSanta C. Mangione, Jun-Song Chen, Kathleen L. Gould
Summary: The coordination of genetic material and cytoplasmic division is mediated by Cdk1, which phosphorylates various substrates including Pxl1. Pxl1 is dephosphorylated by Clp1 and Dis2 at mitotic exit, affecting the recruitment and duration of cytokinesis.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Food Science & Technology
Yen-Tso Lai, Chang-Wei Hsieh, Yi-Chen Lo, Bo-Kang Liou, Hui-Wen Lin, Chih-Yao Hou, Kuan-Chen Cheng
Summary: The study explored the enological characteristics of non-Saccharomyces yeast strains and their influence on aroma compound complexity during fermentation process, finding that they can provide more diverse flavors in wines.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Guihu Zhang, Peng Xiao, Youqiang Xu, Honghua Li, Hehe Li, Jinyuan Sun, Baoguo Sun
Summary: Baijiu, a popular alcoholic beverage, contains over 2000 aroma compounds, with the sulfur-containing compounds being the most influential in shaping its flavor. This study isolated 12 yeast strains capable of producing benzenemethanethiol from baijiu daqu, and identified Saccharomyces cerevisiae strain J14 as the most promising. The optimal fermentation conditions for producing benzenemethanethiol were determined to be 28°C for 24 hours with a 4% inoculum of 3.025 g/L L-cysteine.
Article
Biotechnology & Applied Microbiology
Guokun Wang, Aline Tavares, Simone Schmitz, Lucas Franca, Hugo Almeida, Joao Cavalheiro, Ana Carolas, Suleyman Ozmerih, Lars M. Blank, Bruno S. Ferreira, Irina Borodina
Summary: This study developed an integrated process for manufacturing CCM from glucose by yeast fermentation, achieving high CCM yield and purity. The engineered strain ST10209 produced 1.4 g/L CCM in microplate assay and 22.5 g/L CCM in a 2 L fermenter, outperforming the previous strain ST8943. The CCM recovery yield reached 66.3% with 95.4% purity using a recovery and purification process involving activated carbon treatment.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Ramy S. Yehia, Ahmed M. Saleh, Mohammad Bani Ismail, Saleh Al-Quraishy, Ohoud Al-Amri, Rewaida Abdel-Gaber
Summary: This study investigated the production, characterization, and bioactivity of yeast mannan. The highest production of mannan was observed in the Saccharomyces cerevisiae isolate RY1. Characterization of RY1 mannan revealed its ability to scavenge radicals and exhibit anti-tumor activity against liver and breast cancer cell lines. The findings confirm the potential of S. cerevisiae mannan as a biotherapeutic agent for cancer treatment.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Virology
Mirela Popa, Zsolt Ruzsics, Mark Loetzerich, Lars Doelken, Christopher Buser, Paul Walther, Ulrich H. Koszinowski
JOURNAL OF VIROLOGY
(2010)
Article
Materials Science, Multidisciplinary
Christopher Buser, David G. Drubin
MICROSCOPY AND MICROANALYSIS
(2013)
Article
Multidisciplinary Sciences
Takuma Kishimoto, Yidi Sun, Christopher Buser, Jian Liu, Alphee Michelot, David G. Drubin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2011)
Article
Biochemistry & Molecular Biology
Alexandra Russo, Pragya Goel, E. J. Brace, Chris Buser, Dion Dickman, Aaron DiAntonio
Article
Cell Biology
Pragya Goel, Dominique Dufour Bergeron, Mathias A. Boehme, Luke Nunnelly, Martin Lehmann, Christopher Buser, Alexander M. Waher, Stephan J. Sigrist, Dion Dickman
JOURNAL OF CELL BIOLOGY
(2019)
Article
Biology
Keke Ding, Yifu Han, Taylor W. Seid, Christopher Buser, Tomomi Karigo, Shishuo Zhang, Dion K. Dickman, David J. Anderson
Article
Microbiology
Paul Kelly, Nicholas Backes, Kyle Mohler, Christopher Buser, Arundhati Kavoor, Jesse Rinehart, Gregory Phillips, Michael Ibba
Article
Developmental Biology
Sarah Perry, Pragya Goel, Nancy L. Tran, Cristian Pinales, Christopher Buser, Daniel L. Miller, Barry Ganetzky, Dion Dickman
Article
Cell Biology
Shantae M. Thornton, Varsha D. Samararatne, Joseph G. Skeate, Christopher Buser, Kim P. Luehen, Julia R. Taylor, Diane M. Da Silva, W. Martin Kast
Article
Chemistry, Multidisciplinary
Vincent P. Aguirre, Simonne Jocic, Paul Webster, Christopher Buser, John A. Moss, Laura M. Barge, Yijie Tang, Yisong Guo, Marc M. Baum
Summary: Green rust is an important chemically reduced mineral that may have played a role in the geochemical cycles of early Earth and Mars. Strictly anaerobic synthesis and protecting these oxygen-sensitive samples can be challenging.
ACS EARTH AND SPACE CHEMISTRY
(2021)
Article
Pathology
Xuan Bao, Zhaoxia Zhang, Yanjiang Guo, Christopher Buser, Harold Kochounian, Nancy Wu, Xiaohua Li, Shikun He, Bin Sun, Fred N. Ross-Cisneros, Alfredo A. Sadun, Lvzhen Huang, Mingwei Zhao, Henry K. W. Fong
Summary: Age-related macular degeneration is a progressive eye disease that is the leading cause of blindness in elderly individuals. The disease is characterized by the formation of extracellular deposits known as drusen between the retinal pigment epithelium and Bruch membrane. Research suggests that abnormal RGR proteins, such as RGR-d, may play a pathogenic role in the degeneration of the choriocapillaris, retinal pigment epithelium, and photoreceptors, leading to potential vision loss.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Cell Biology
Yi-Jheng Peng, Junhua Geng, Ying Wu, Cristian Pinales, Jennifer Langen, Yen-Ching Chang, Christopher Buser, Karen T. Chang
Summary: The study identifies Mnb and Synj as novel regulators of ADBE, and reveals that CME and ADBE are differentially governed by the dual phosphatase domains of Synj.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Manjula Gunawardana, Mariana Remedios-Chan, Debbie Sanchez, Simon Webster, Amalia E. Castonguay, Paul Webster, Christopher Buser, John A. Moss, MyMy Trinh, Martin Beliveau, Craig W. Hendrix, Mark A. Marzinke, Michael Tuck, Richard M. Caprioli, Michelle L. Reyzer, Joseph Kuo, Philippe A. Gallay, Marc M. Baum
Summary: Global efforts to prevent HIV-1 infection in vulnerable populations are stagnant, while the development of long-acting subdermal implants delivering the potent prodrug, TAF, shows significant potential. In this study, using mouse models and imaging mass spectrometry, we address knowledge gaps in the preclinical pharmacology of long-acting subdermal TAF delivery and determine the effective TAF dose for preventing HIV-1 acquisition.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Alberto Danese, Simone Patergnani, Alessandra Maresca, Camille Peron, Andrea Raimondi, Leonardo Caporali, Saverio Marchi, Chiara La Morgia, Valentina Del Dotto, Claudia Zanna, Angelo Iannielli, Alice Segnali, Ivano Di Meo, Andrea Cavaliere, Magdalena Lebiedzinska-Arciszewska, Mariusz R. Wieckowski, Andrea Martinuzzi, Milton N. Moraes-Filho, Solange R. Salomao, Adriana Berezovsky, Rubens Belfort, Christopher Buser, Fred N. Ross-Cisneros, Alfredo A. Sadun, Carlo Tacchetti, Vania Broccoli, Carlotta Giorgi, Valeria Tiranti, Valerio Carelli, Paolo Pinton
Summary: Patients with Leber's hereditary optic neuropathy (LHON) exhibit sustained abnormal autophagy and compartment-specific mitophagy activity, disrupting mitochondrial homeostasis and leading to defective bioenergetics and excessive reactive oxygen species production. Modulating autophagy and mitochondrial biogenesis can counteract this pathological mechanism.
Article
Biology
Yifu Han, Chun Chien, Pragya Goel, Kaikai He, Cristian Pinales, Christopher Buser, Dion Dickman
Summary: In this study, the researchers used the Drosophila larval neuromuscular junction as a model system to separate neurotransmission between tonic and phasic neurons using botulinum neurotoxin BoNT-C. They also identified heterosynaptic plasticity rules in this circuit.
