Article
Developmental Biology
Philipp M. Tschaikner, Dominik Regele, Ruth Roeck, Willi Salvenmoser, Dirk Meyer, Michel Bouvier, Stephan Geley, Eduard Stefan, Pia Aanstad
Summary: In this study, Gpr161 was found to play a crucial role in negatively regulating the Hh signaling pathway by activating PKA to inhibit the expression of Hh target genes. Additionally, PKA phosphorylation-deficient forms of Gpr161 were shown to increase sensitivity to Shh, resulting in excessive high-level Hh signaling. These findings suggest that PKA-mediated phosphorylation of Gpr161 may provide a mechanism for fine-tuning Gpr161 ciliary localization and PKA activity.
Article
Multidisciplinary Sciences
Ricardo Fradique, Erika Causa, Clara Delahousse, Jurij Kotar, Laetitia Pinte, Ludovic Vallier, Marta Vila-Gonzalez, Pietro Cicuta
Summary: Cilia density, distribution, and beating frequency are crucial properties of airway epithelial tissues. This article introduces a novel and open-source method for fully characterizing cilia beating frequency and motile cilia coverage in an automated manner without any user intervention. The method successfully differentiates between different coverage densities, identifies small patches of cilia in larger fields of view, and provides a comprehensive characterization of cilia beating frequency in all moving areas.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Editorial Material
Cell Biology
Ivan Olaya, Sean M. Burgess
Summary: A recent study found that a cilium in zebrafish acts as an anchor for the centrosome and is crucial for telomere clustering and germ cell development.
DEVELOPMENTAL CELL
(2022)
Review
Cell Biology
Maulin M. Patel, Leonidas Tsiokas
Summary: The primary cilium is an important organelle present in most cell types, serving as a signaling center for various cellular processes. Abnormal cilia length can lead to a range of human diseases, known as ciliopathies. While cilia assembly is well understood, the process of cilia disassembly and its biological roles remain less clear and require further investigation for potential therapeutic targets.
Review
Cell Biology
Ashish K. Maurya
Summary: Sensory cilia is an ancient organelle with highly conserved structure and functioning. They serve a wide range of sensory functions and exhibit diverse shapes and sizes to fulfill specialized roles. Studying the structural diversity and underlying mechanisms of cilia can provide a comprehensive understanding of ciliary biology and uncover novel aspects of ciliary disease biology.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biology
Jingliang Zhang, Chenyu Zhang, Xiaoling Chen, Bingwei Wang, Weining Ma, Yang Yang, Ruimao Zheng, Zhuo Huang
Summary: Temporal lobe epilepsy (TLE) is a common and intractable neurological disorder, with dysfunctional PKA signaling playing a role in its development. A study found that reduced autophosphorylation of the PKA-RIIβ subunit is associated with decreased PKA activity and altered neuronal excitability, suggesting it as a potential therapeutic target for TLE.
COMMUNICATIONS BIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Siebren Faber, Ronald Roepman
Summary: This article introduces an optimized expansion microscopy for retinal tissue, which represents a significant advancement in studying inherited blindness.
Article
Cell Biology
Zahra Chinipardaz, Gongsheng Yuan, Min Liu, Dana T. Graves, Shuying Yang
Summary: Foxo1 upregulation is associated with impaired fracture healing in diabetic conditions. The mechanism by which diabetes causes cilia loss during fracture healing remains unclear.
CELL DEATH DISCOVERY
(2023)
Review
Biochemistry & Molecular Biology
Jesus Eduardo Martin-Salazar, Diana Valverde
Summary: Primary cilia, non-motile organelles associated with the cell cycle, are formed through various mechanisms and are linked to gene complexes. Genetic defects can lead to faulty cilium formation and the occurrence of ciliopathies.
Article
Biochemical Research Methods
Stef Broeren, Ines Figueiredo Pereira, Tongsheng Wang, Jaap den Toonder, Ye Wang
Summary: Researchers have developed an on-demand micromixer based on magnetic microwalls, which can achieve controllable mixing and complete mixing shut-off in commercial microfluidic devices. The microwalls can create a meandering path in the main channel to enable mixing or open up the channel to stop mixing, controlled by a simple magnet. Experimental results show that the microwalls can effectively enhance mixing in colored fluids with different flow rates and viscosities.
Article
Pharmacology & Pharmacy
Jialu Wu, Yue Wan, Zhenxing Wu, Shengyu Zhang, Dongsheng Cao, Chang-Yu Hsieh, Tingjun Hou
Summary: MF-SuP-pKa is a novel pKa prediction model that utilizes subgraph pooling, multi-fidelity learning, and data augmentation. The model captures the local and global environments around ionization sites for micro-pKa prediction using a knowledge-aware subgraph pooling strategy. By fitting low-fidelity data to high-fidelity data through transfer learning, MF-SuP-pKa achieves superior performance compared to state-of-the-art models with less high-fidelity training data.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Physics, Applied
Hideyuki Sugioka, Wataru Tomita, Mitsuhiro Tanaka
Summary: The study demonstrates that a thermally actuated double pendulum can exhibit a metachronal motion using a self-propulsion mechanism caused by spontaneous asymmetrical heat transfer. By using a multiple pendulum structure with beams of different lengths, phase differences can be produced among the beams. This device has potential applications in biomedical microfluidic systems or microrobots in water.
JOURNAL OF APPLIED PHYSICS
(2021)
Review
Cell Biology
Zhanhong Ren, Xiaoxiao Mao, Siqi Wang, Xin Wang
Summary: This article reviews the characteristics, molecular mechanisms, and treatment methods of ciliopathy and primary ciliary dyskinesia (PCD), emphasizing the importance of further research on cilia-related diseases.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Jay Gopalakrishnan, Kerstin Feistel, Benjamin M. Friedrich, Anne Grapin-Botton, Nathalie Jurisch-Yaksi, Elvira Mass, David U. Mick, Roman-Ulrich Mueller, Helen May-Simera, Bernhard Schermer, Miriam Schmidts, Peter Walentek, Dagmar Wachten
Summary: Primary cilia are dynamic cellular protrusions that play a crucial role in sensing and processing extracellular signals, controlling cell fate, and function during tissue development.
Review
Biochemistry & Molecular Biology
Lance Lee, Lawrence E. Ostrowski
Summary: Over the past two decades, our understanding of motile cilia and their role in disease has greatly increased, with critical information and insight coming from the analysis of mouse models. Mouse models have made significant contributions to genetic and cell biological studies of motile cilia, and have been at the forefront of research on mammalian motile cilia and PCD pathogenesis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sarah E. Fiedler, Amelia R. Kerns, Catherine Tsang, Vivian Tsang, Dennis Bourdette, Sonemany Salinthone
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Cell Biology
Maxfield P. Flynn, Sarah E. Fiedler, Amelia B. Karlsson, Daniel W. Carr, Evelyn T. Maizels, Mary Hunzicker-Dunn
JOURNAL OF CELL SCIENCE
(2016)
Article
Neurosciences
Sarah E. Fiedler, Vijayshree Yadav, Amelia R. Kerns, Catherine Tsang, Sheila Markwardt, Edward Kim, Rebecca Spain, Dennis Bourdette, Sonemany Salinthone
MOLECULAR NEUROBIOLOGY
(2018)
Article
Reproductive Biology
Sarah E. Fiedler, Malini Bajpai, Daniel W. Carr
BIOLOGY OF REPRODUCTION
(2008)
Article
Reproductive Biology
Sarah E. Fiedler, Tejasvi Dudiki, Srinivasan Vijayaraghavan, Daniel W. Carr
BIOLOGY OF REPRODUCTION
(2013)
Article
Cell Biology
Sarah E. Fiedler, Joseph H. Sisson, Todd A. Wyatt, Jacqueline A. Pavlik, Todd M. Gambling, Johnny L. Carson, Daniel W. Carr
Article
Biochemistry & Molecular Biology
Sarah E. Fiedler, Robynn V. Schillace, Crystal J. Daniels, Sarah F. Andrews, Daniel W. Carr
Article
Multidisciplinary Sciences
Mary E. Hunzicker-Dunn, Blanca Lopez-Biladeau, Nathan C. Law, Sarah E. Fiedler, Daniel W. Carr, Evelyn T. Maizels
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Cell Biology
Sarah E. Fiedler, Rebecca Spain, Edward Kim, Sonemany Salinthone
Summary: Lipoic acid (LA) exhibits antioxidant and anti-inflammatory effects in multiple sclerosis (MS) by modulating inflammatory cytokine secretion and phagocytosis, potentially via a cAMP-mediated mechanism.
IMMUNOLOGY AND CELL BIOLOGY
(2021)
Article
Plant Sciences
NN Gushwa, D Hayashi, A Kemper, B Abram, JE Taylor, J Upton, CF Tay, S Fiedler, S Pullen, LP Miller, G Tallman
Article
Reproductive Biology
M Bajpai, SE Fiedler, ZH Huang, S Vijayaraghavan, GE Olson, G Livera, M Conti, DW Carr
BIOLOGY OF REPRODUCTION
(2006)