Journal
BIOMEDICAL OPTICS EXPRESS
Volume 5, Issue 3, Pages 778-787Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.5.000778
Keywords
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Funding
- European Union
- State of Hungary
- European Social Fund [TAMOP-4.2.4.A/2-11/1-2012-0001, TAMOP-4.2.2.C-11/1/KONV-2012-0010]
- Impulse lasers for use in materials science and biophotonics [TAMOP-4.2.2.A-11/ 1/KONV-2012-0060]
- Chemical and Biological Metrology programme of the UK National Measurement Office
- Marie Curie Integration Grant [PCIG13-GA-2013-618273]
- Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
- EPSRC [EP/H018301/1] Funding Source: UKRI
- Alzheimers Research UK [ARUK-EG2012A-1, ARUK-PG2013-14] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/H018301/1] Funding Source: researchfish
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Localization-based super-resolution microscopy image quality depends on several factors such as dye choice and labeling strategy, microscope quality and user-defined parameters such as frame rate and number as well as the image processing algorithm. Experimental optimization of these parameters can be time-consuming and expensive so we present TestSTORM, a simulator that can be used to optimize these steps. TestSTORM users can select from among four different structures with specific patterns, dye and acquisition parameters. Example results are shown and the results of the vesicle pattern are compared with experimental data. Moreover, image stacks can be generated for further evaluation using localization algorithms, offering a tool for further software developments. (C) 2014 Optical Society of America
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