Article
Optics
Denise Valente, Kari V. Vienola, Robert J. Zawadzki, Ravi S. Jonnal
Summary: The study introduces a novel method of multiplexing FF-SS-OCT signals using carrier modulation (CM), which allows for simultaneous measurement of differential scattering anisotropy in the x and y dimensions. By utilizing different optical pupils to acquire images passing through, the technique demonstrates the potential for inspecting various properties of scattered light.
Article
Neurosciences
Tylor R. Lewis, Mustafa S. Makia, Carson M. Castillo, Muayyad R. Al-Ubaidi, Muna Naash, Vadim Y. Arshavsky
Summary: Mutations in the PRPH2 gene cause a broad range of retinal diseases by affecting the structure of outer segment of photoreceptor cells, resulting in irregular disc enclosure process. Abnormal PRPH2 oligomerization leads to defects in outer segment structure, particularly at the stage of disc enclosure.
JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Ailis L. Moran, John D. Fehilly, Daniel Floss Jones, Ross Collery, Breandan N. Kennedy
Summary: Outer segment phagocytosis (OSP) is a regulated process in which photoreceptor outer segment tips are consumed by adjacent retinal pigment epithelium (RPE) cells. However, there is still limited knowledge about the key regulators of OSP in vivo. Understanding the regulation of OSP is crucial for the treatment of ocular diseases related to OSP.
Article
Multidisciplinary Sciences
Erin H. H. Su, Niranjana Kesavamoorthy, Hossein Ameri
Summary: Studies have shown that prolonged dark adaptation does not cause significant changes in the thickness, volume, or intensity of the outer or overall retina. These findings challenge our current understanding of the neuroprotective effects of dark adaptation in preventing blindness and highlight the need for further research.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Dianlei Guo, Jiali Ru, Lijing Xie, Mingjuan Wu, Yingchun Su, Shiyong Zhu, Shujuan Xu, Bin Zou, Yanhong Wei, Xialin Liu, Yizhi Liu, Chunqiao Liu
Summary: The study discovered that Tmem138 plays a crucial role in the functional organization of photoreceptor connecting cilium (CC), which is essential for rhodopsin localization and outer segment (OS) biogenesis. Inactivation of Tmem138 leads to abnormal development of OS and degeneration of photoreceptor cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemical Research Methods
Nikolai P. P. Skiba, Tylor R. R. Lewis, William J. J. Spencer, Carson M. M. Castillo, Andrej Shevchenko, Vadim Y. Y. Arshavsky
Summary: We used quantitative mass spectrometry to determine the abundances of 20 key proteins in mouse rod outer segments, and calculated the absolute number of molecules and molar ratios for each protein. This study resolved discrepancies in the reported abundances of these proteins and advanced our understanding of the coordinated molecular function of the phototransduction pathway.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nemanja Milicevic, Ouafa Ait-Hmyed Hakkari, Udita Bagchi, Cristina Sandu, Aldo Jongejan, Perry D. Moerland, Jacoline B. ten Brink, David Hicks, Arthur A. Bergen, Marie-Paule Felder-Schmittbuhl
Summary: The study showed that Per1 and Per2 are essential clock components for driving photoreceptor outer segment phagocytosis, and this process may be transcriptionally driven by the retinal pigment epithelium (RPE). No differentially expressed genes were found in photoreceptors, while several genes were differentially expressed in the RPE, both in wild type and Per1, Per2 double mutant mice. STRING analysis identified a group of interacting genes potentially involved in driving photoreceptor outer segment phagocytosis in the RPE.
Article
Multidisciplinary Sciences
William J. Spencer, Nicholas F. Schneider, Tylor R. Lewis, Carson M. Castillo, Nikolai P. Skiba, Vadim Y. Arshavsky
Summary: The photoreceptor outer segment is a cilium filled with flat-tened disc membranes for light capture. The addition of new discs at the base of outer segments is driven by Arp2/3-mediated actin polymerization initiated by the pentameric WAVE complex. Knockout of WASF3, a subunit of the WAVE complex, disrupts actin polymerization and leads to disorganized membrane lamellae instead of a proper outer segment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Matthias Poege, Julia Mahamid, Sanae S. Imanishi, Juergen M. Plitzko, Krzysztof Palczewski, Wolfgang Baumeister
Summary: Studies have identified two molecular connectors/spacers in the membrane structure of rod outer segment (ROS) that likely contribute to the precise stacking of ROS disks. Additionally, a continuous supramolecular assembly composed of peripherin-2 (PRPH2) and rod outer segment membrane protein 1 (ROM1) oligomers enforces the extreme radius of curvature at the disk rims. Together, these molecular assemblies constitute the structural basis of the highly specialized ROS functional architecture.
Article
Neurosciences
Jillian N. Pearring, Jorge Martinez-Marquez, Jason R. Willer, Eric C. Lieu, Raquel Y. Salinas, Vadim Y. Arshavsky
Summary: The study found that the targeted delivery of the rod cyclic nucleotide-gated (CNG) channel to the outer segment uses the conventional secretory pathway and requires preassembly of its constituent alpha 1 and beta 1 subunits. The N-terminal glutamic acid-rich protein domain of CNG beta 1 encodes specific information targeting the channel to rod outer segments and connects the channel to photoreceptor disk rims through an interaction with peripherin-2. These findings reveal fine functional specializations within the CNG channel's structural domains and suggest that its sequestration to the outer segment plasma membrane requires an interaction with peripherin-2.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Daniel E. Maidana, Lucia Gonzalez-Buendia, Joan W. Miller, Demetrios G. Vavvas
Summary: Recent evidence suggests that modulating the RIPK3 pathway can improve the survival of transplanted cells and aid in the repair of degenerated retinas. Simultaneous inhibition of RIPK3 in both the donor and host can maximize the survival of transplanted cells. Additionally, targeting RIPK3 in the immune system can protect both donor and host retinal cells. These findings highlight the potential of immunomodulatory and neuroprotective strategies targeting RIPK3 in the regenerative therapy of photoreceptor transplantation.
Article
Neurosciences
Una Greferath, Mario Huynh, Andrew Ian Jobling, Kirstan Anne Vessey, Gene Venables, Denver Surrao, Helen Christine O'Neill, Ioannis J. Limnios, Erica Lucy Fletcher
Summary: Retinitis pigmentosa is a group of inherited retinal degenerations characterized by gradual loss of photoreceptors, ultimately leading to irreversible vision loss. This study focused on analyzing the changes in glial, microglial, and photoreceptor cells in different retinal locations in RCS rats, revealing significant differences in photoreceptor loss between the dorsal and ventral retina. The findings suggest that breakdown of the outer limiting membrane may play a role in exacerbating photoreceptor loss in the ventral retina, highlighting the value of using the RCS rat model for sectorial retinitis pigmentosa.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemical Research Methods
Shuibin Ni, Shanjida Khan, Thanh-Tin P. Nguyen, Ringo Ng, Brandon J. Lujan, Ou Tan, David Huang, Yifan Jian
Summary: This paper introduces a novel imaging technology called Volumetric Directional OCT (VD-OCT) that can dynamically adjust the incident beam position during scanning, allowing for early identification of retinal diseases.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Chemistry, Multidisciplinary
Yongan Feng, Jie Wang, Zhimin Li
Summary: This study introduces a novel strategy for designing layered high-energy materials by controlling the directional hydrogen bonding, allowing energetic molecules to stack layer by layer to form a target structure. The three substances designed using this strategy exhibit expected layered structures and demonstrate excellent insensitivity and detonation performance.
CRYSTAL GROWTH & DESIGN
(2021)
Article
Cell Biology
Ashish K. Solanki, Manas R. Biswal, Stephen Walterhouse, Rene Martin, Altaf A. Kondkar, Hans-Joachim Knolker, Bushra Rahman, Ehtesham Arif, Shahid Husain, Sandra R. Montezuma, Deepak Nihalani, Glenn Prazere Lobo
Summary: The unconventional myosin MYO1C plays a crucial role in rhodopsin localization to photoreceptor outer segments and is essential for normal visual function. Loss of MYO1C in mice leads to rhodopsin mislocalization, impaired photoreceptor function, and progressively shorter outer segments, indicating its significance in retinal cell physiology.
Article
Biochemical Research Methods
Alessandra Carmichael Martins, Brian Vohnsen
BIOMEDICAL OPTICS EXPRESS
(2019)
Article
Biochemical Research Methods
Vinay Kumar Nilagiri, Marwan Suheimat, Andrew J. Lambert, Andrew Turpin, Brian Vohnsen, David A. Atchison
Summary: The Stiles-Crawford effect of the first kind (SCE) refers to the phenomenon where light entering the eye near the center of the pupil appears brighter than light entering near the edge. Research showed that SCE directionality increases as the testing location moves from the center to parafoveal positions, and this effect is influenced by field size. Using a uniaxial Maxwellian system, it was found that SCE directionality increases with larger field sizes, supported by a geometric optics absorption model.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Neurosciences
Brian Vohnsen
Summary: This study explores the relationship between vergence, age-dependent scaling of eye and photoreceptor parameters, and the angular spectrum of waves incident on the eye pupil. The research suggests that a pupil size limitation of 3mm exists for children in the school age, as larger pupils may lead to leakage and crosstalk among photoreceptors. Balancing photoreceptor outer segment length and density in relation to pupil size is crucial for optimal light capture efficiency and prevention of leakage and crosstalk.
Editorial Material
Optics
Juan Tabernero, Linda Lundstrom, Christina Schwarz, Brian Vohnsen
Summary: This feature issue brings together articles from the Visual and Physiological Optics meeting (VPO2022) held in August 2022 in Cambridge, UK. It showcases a range of topics discussed at the meeting and provides examples of current research in the field, published jointly by Biomedical Optics Express and the Journal of the Optical Society of America A.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Biochemical Research Methods
Juan Tabernero, Linda Lundstroem, Christina Schwarz, Brian Vohnsen
Summary: This feature issue gathers articles presented at the tenth Visual and Physiological Optics meeting (VPO2022) that took place on August 29-31, 2022, in Cambridge, UK. It is a joint feature issue between Biomedical Optics Express and Journal of the Optical Society of America A, covering a wide range of topics discussed at the meeting and providing examples of current research in the field.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Chemistry, Multidisciplinary
Najnin Sharmin, Petros Papadogiannis, Dmitry Romashchenko, Linda Lundstrom, Brian Vohnsen
Summary: The accommodative response of the human eye is predominantly driven by foveal vision but also reacts to off-foveal stimuli. This study measured monocular accommodation using annular stimuli centered around the fovea and extending to different radial eccentricities. The results showed a significant reduction in accommodative response with increased radial eccentricity.
APPLIED SCIENCES-BASEL
(2023)
Meeting Abstract
Ophthalmology
Najnin Sharmin, Brian Vohnsen
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Meeting Abstract
Ophthalmology
Brian Vohnsen, Qi Qi Kennedy, Najnin Sharmin
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Proceedings Paper
Optics
B. Vohnsen
Summary: The impact of different Zernike aberration terms on the retina is discussed in this paper, with even-ordered Zernike aberrations having a wavefront slope at the retina while odd-ordered Zernike aberration modes do not have wavefront tilt across the point-spread function. Understanding this is essential for how vision is triggered in normal viewing conditions as well as for analyzing vision and photoreceptors in ophthalmic design using psychophysical methods.
CURRENT DEVELOPMENTS IN LENS DESIGN AND OPTICAL ENGINEERING XXII
(2021)
Meeting Abstract
Ophthalmology
Brian Vohnsen
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Proceedings Paper
Ophthalmology
N. Sharmin, B. Vohnsen
LIGHT IN NATURE VIII
(2020)
Proceedings Paper
Ophthalmology
B. Vohnsen
LIGHT IN NATURE VIII
(2020)
Meeting Abstract
Ophthalmology
Brian Vohnsen, Alessandra Carmichael Martins, Najnin Sharmin, Salihah Qaysi
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Salihah Qaysi, Brian Vohnsen
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Meeting Abstract
Ophthalmology
Najnin Sharmin, Petros Papadogiannis, Dimitry Romaschenko, Linda Lundstrom, Brian Vohnsen
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
