Article
Chemistry, Analytical
Diwakar M. Awate, Seth Holton, Katherine Meyer, Jaime J. Juarez
Summary: Flow focusing is an important technique for studying cellular samples, and this article presents low-cost millifluidic flow-focusing devices fabricated using a desktop 3D printer. Two printing strategies, monolithic SLA and hybrid molding, were used to create the devices. The experiments showed that the focusing widths achieved in these devices can be validated using a numerical model developed in ANSYS.
Article
Chemistry, Analytical
Chengxin Wu, Xing Wei, Xue Men, Yulong Xu, Junjie Bai, Yu Wang, Lei Zhou, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: We constructed an open and low-cost flow cytometer that integrates single cell aligning and fluorescence detection functions. The hardware cost for the LIF detection unit and 3D focusing device is $3200 and $400 respectively. The flow cytometer showed favorable assay precision and accuracy, and was successfully applied for evaluating ROS generation in single HepG2 cells.
Article
Nanoscience & Nanotechnology
Xiaofei Yuan, Andrew Glidle, Hitoshi Furusho, Huabing Yin
Summary: Traditional two-dimensional fluid control strategies in microfluidic cell sorting have limitations in terms of precision and purity, while devices based on three-dimensional hydrodynamics offer better flow-focusing characteristics but are restricted by arbitrary sample positioning. The 3D hydrodynamic focusing sorting platform designed in this study has advantages in precisely controlling sample velocity and position, showing potential for high-accuracy Raman activated sorting.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Optics
Brett F. Bathel, Joshua M. Weisberger
Summary: The novel schlieren system presented in this study eliminates the need for separate source and cutoff grids by manipulating the polarization of light through a single grid element, reducing complexity and setup time. The system's sensitivity to density objects can be adjusted using a polarizing prism, and it has been shown to be useful for wind tunnel measurements with images taken through acrylic windows.
Article
Chemistry, Multidisciplinary
Guantong Wang, Masaki Kudo, Kazuho Daicho, Sivasankaran Harish, Bin Xu, Cheng Shao, Yaerim Lee, Yuxuan Liao, Naoto Matsushima, Takashi Kodama, Fredrik Lundell, L. Daniel Soederberg, Tsuguyuki Saito, Junichiro Shiomi
Summary: This study demonstrates that nanocellulose materials can exhibit high thermal conductivity when their nanofibrils are highly aligned and bonded. The crystallinity of the filaments significantly influences their thermal conductivity.
Article
Chemistry, Analytical
Dayananda Desagani, Shani Kleiman, Teddy Zagardan, Hadar Ben-Yoav
Summary: Three-dimensional-printed lab-on-a-chip (LOC) devices offer cost-efficient and easy-to-handle solutions for particle analysis, allowing for remote diagnosis and monitoring of medical conditions such as inflammation.
Article
Biochemical Research Methods
Wenhan Zhao, Xiaopeng Shang, Boran Zhang, Dan Yuan, Binh Thi Thanh Nguyen, Wenshuai Wu, Jing Bo Zhang, Niancai Peng, Ai Qun Liu, Fei Duan, Lip Ket Chin
Summary: This study systematically investigates the flow transition process in the hydrodynamic focusing mechanism, revealing the transition from the squeezed focusing state to the over-squeezed anti-focusing state. The recovery rate of cell analysis can be maintained by limiting the Reynolds number below 30. These findings provide new insights into microfluidic cytometric analyses with various applications in food safety, water monitoring, and healthcare sectors.
Article
Optics
Xing Li, Yang Shi, Ting Pan, Dengyun Lu, Guoshuai Zhu, Jianyun Xiong, Danning Wang, Ziyi He, Jingping Huang, Hongbao Xin
Summary: A convenient opto-hydrodynamic strategy for 3D dynamic microswarm actuation based on the photothermal gradient-induced Marangoni effect is proposed. Such microswarm petals are controllably reconfigurable, deformable, and capable of performing stable migration. Importantly, this opto-hydrodynamic strategy is applicable for the formation of artificial 3D-dynamic bio-microswarms using different biological cells, which further facilitate the regulation of biological processes such as bacteria growth/division.
LASER & PHOTONICS REVIEWS
(2023)
Article
Multidisciplinary Sciences
Filippo Storti, Silvio Bonfadini, Luigino Criante
Summary: Accurately controlling the position of fluid and particles is crucial for lab-on-a-chip platforms. The development of microfluidic fabrication technology has enabled three-dimensional hydrodynamic focusing, which has revolutionized single-cell analysis systems. However, there is still a lack of a device that meets the requirements of high throughput, compactness, high integrability, and ease of use for biomedical research and clinical applications. In this study, a 3D flow focusing microfluidic device buried in fused silica substrate was proposed, which shows promising advantages in terms of focusing capability. High accuracy and resolution were achieved in the detection of particles and bacteria.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Roni Sverdlov Arzi, Asaf Kay, Yulia Raychman, Alejandro Sosnik
Summary: Nanoprecipitation and microfluidics technology are effective methods for producing pure drug nanoparticles and optimizing product performance. The use of specific microfluidic devices can lead to the production of rounded, optimized nanoparticles.
Article
Engineering, Chemical
Yaser Kazemi, Arman Sadeghi, Abdullah Irankhah
Summary: Keeping reactants close to the reactive wall is crucial in microreactors where surface reactions are dominant. Single-phase hydrodynamic focusing is an effective technique, but has the drawback of high penetration of reactants into the sheath fluid. To overcome this, the concept of two-phase hydrodynamic focusing is introduced, using a highly viscous sheath fluid to create a barrier against reactant penetration.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Chemistry, Analytical
Zixi Chao, Yong Han, Zeheng Jiao, Zheng You, Jingjing Zhao
Summary: Flow cytometers are instruments used for rapid quantitative analysis of cell suspension. Traditional flow cytometry uses multi-channel filters to detect fluorescence, but full-spectrum fluorescence based on dispersion detection is a more effective and accurate method. Prism dispersion exhibits higher and more uniform light energy utilization, making it a more suitable method for small flow cytometers. The design criteria for prism dispersion fluorescence detection were demonstrated and verified in this study.
Article
Chemistry, Multidisciplinary
Michael. A. Warren, Amir Shakouri, Victor Pacheco-Pena, Toby Hallam
Summary: This paper introduces a novel design for microfluidic impedance cytometry devices that enhances the accuracy in determining the size of cells by producing a homogeneous electric field in the sensing region. It reduces the reliance on cell focusing and signal postprocessing, demonstrating higher accuracy compared to traditional designs in cell counting and sizing. The concept is validated through finite element simulations to generate suitable data sets for particle trajectories and model expected signal variations.
Article
Engineering, Electrical & Electronic
Yogesh M. Patel, Ritika Malik, Kedar Khare, Supreet Singh Bahga
Summary: We propose a microfluidic holographic cytometry technique that uses three-dimensional hydrodynamic focusing to accurately visualize, classify, and quantify cells and particles in a mixture. This technique achieves high-resolution holographic imaging without the need for computationally-expensive numerical refocusing used in existing methods. It also prevents cell clustering and can be fabricated at a low cost. The technique shows promise for label-free classification and quantification of infected cells in various applications.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2023)
Article
Mechanics
Nathan J. J. Di Vaira, Lukasz Laniewski-Wollk, Raymond L. L. Johnson, Saiied M. M. Aminossadati, Christopher R. R. Leonardi
Summary: Hydrodynamic clogging in planar channels is investigated using direct numerical simulation, focusing on the influence of electrostatic forces. The research incorporates electrostatic physics into an existing numerical framework and verifies the results. It is found that the clogging probability depends on the Stokes number at different ranges. Under the effect of electrostatic forces, clogging is represented by the wall adhesion number. The study also reveals the impact of particle agglomeration and channel width on clogging, and the sensitivities to the Coulomb friction coefficient.
JOURNAL OF FLUID MECHANICS
(2023)
Article
Engineering, Electrical & Electronic
Pasquale Imperatore, Gianluca Persichetti, Genni Testa, Romeo Bernini
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2020)
Article
Optics
Genni Testa, Gianluca Persichetti, Romeo Bernini
Summary: The full polymeric optofluidic FP resonator has a high quality factor and sensitivity for refractometric sensing applications. The device is based on multilayer optical polymeric films, simplifying fabrication process while maintaining high optical surface quality.
Article
Biochemical Research Methods
Sara Tombelli, Cosimo Trono, Simone Berneschi, Chiara Berrettoni, Ambra Giannetti, Romeo Bernini, Gianluca Persichetti, Genni Testa, Guillermo Orellana, Francesca Salis, Susanne Weber, Peter B. Luppa, Giampiero Porro, Giovanna Quarto, Markus Schubert, Marcel Berner, Paulo P. Freitas, Susana Cardoso, Fernando Franco, Vania Silverio, Maria Lopez-Martinez, Urs Hilbig, Kathrin Freudenberger, Gunter Gauglitz, Holger Becker, Claudia Gartner, Mark T. O'Connell, Francesco Baldini
Summary: This paper describes a compact point of care optical device for therapeutic drug monitoring, utilizing immunoassays in disposable plastic chips to detect multiple analytes simultaneously with high efficiency. The device is equipped with a microfluidic system and thin film amorphous silicon photodiodes for fluorescence detection, improving the speed and sensitivity of the assay.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Electrical & Electronic
Aldo Minardo, Luigi Zeni, Romeo Bernini, Ester Catalano, Raffaele Vallifuoco
Summary: This paper demonstrates quasi-distributed refractive index sensing using multiple tapers in optical fibers, showing the variation of the Brillouin frequency shift with the diameter of the tapered fiber and the outer refractive index in an optical fiber taper. It also discusses the possibility of multi-point refractive index sensing along the same fiber.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2022)
Article
Engineering, Electrical & Electronic
Pasquale Imperatore, Genni Testa, Gianluca Persichetti, Romeo Bernini
Summary: The power coupling between two parallel oriented, equal length light diffusing multimodal optical fibers, coupled through scattering processes, is investigated both theoretically and experimentally. A simple analytical model of the inherent coupling coefficient is developed using a perturbation approach. The results of the model are compared with experimental measurements at three different wavelengths, showing a close agreement. These findings provide a better understanding of the power coupling mechanism and the dependence on the main structural parameters of the two-fiber configuration.
JOURNAL OF LIGHTWAVE TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Genni Testa, Gianluca Persichetti, Romeo Bernini
Summary: We have presented an optofluidic hybrid silicon-polymer planar ring resonator with integrated microfluidic channels. The low-loss integration of microfluidic channels was achieved by utilizing the interference pattern created by the self-imaging effect. Numerical simulations were conducted to minimize the propagation losses along the ring loop caused by the integration of microfluidic channels. Optical characterization showed that the proposed approach is suitable for microfluidics integration in planar optofluidic design.
Article
Optics
E. Catalano, R. Vallifuoco, R. Bernini, L. Zeni, A. Minardo
Summary: This paper demonstrates the use of non-adiabatic tapers in optical fibers for refractive index sensing based on Brillouin scattering. By exciting higher order optical modes, a multipeaked Brillouin gain spectrum is achieved, resulting in an enhanced sensitivity to refractive index changes. The spectral difference between two peaks also allows for a temperature-independent measurement of the external refractive index.
Article
Engineering, Electrical & Electronic
Gianluca Persichetti, Genni Testa, Pasquale Imperatore, Romeo Bernini
Summary: An optical fiber sensor system for distributed optical absorption spectroscopy based on light diffusing optical fiber is proposed and evaluated. The system allows distributed measurements of optical absorption properties of the sample medium between the fibers, with a spatial resolution of about 17 cm over a 4 m measurement range. The system has also been used for distributed chemical sensing, achieving a limit of detection of 710 ppm for water solutions containing copper ions.
IEEE SENSORS JOURNAL
(2023)
Review
Chemistry, Analytical
Aldo Minardo, Romeo Bernini, Gaia Maria Berruti, Giovanni Breglio, Francesco Antonio Bruno, Salvatore Buontempo, Stefania Campopiano, Ester Catalano, Marco Consales, Agnese Coscetta, Andrea Cusano, Maria Alessandra Cutolo, Pasquale Di Palma, Flavio Esposito, Francesco Fienga, Michele Giordano, Antonio Iele, Agostino Iadicicco, Andrea Irace, Mohammed Janneh, Armando Laudati, Marco Leone, Luca Maresca, Vincenzo Romano Marrazzo, Marco Pisco, Giuseppe Quero, Michele Riccio, Anubhav Srivastava, Patrizio Vaiano, Luigi Zeni, Antonello Cutolo
Summary: Our research group, made up of researchers from various universities in Campania, Italy, has been dedicated to studying photonic sensors for safety and security applications in healthcare, industry, and the environment for the past twenty years. This paper is the first in a series of three and it presents the main concepts of the technologies used in our photonic sensors and reviews our key findings in innovative applications for infrastructure and transportation monitoring.
Article
Optics
E. Catalano, R. Vallifuoco, R. Bernini, L. Zeni, A. Minardo
Summary: In this manuscript, it is demonstrated that an array of tapers formed along a single-mode optical fiber can be used for localized, dynamic strain sensing position with a Brillouin Optical Frequency-Domain Analysis (BOFDA) sensor. The periodic modulation of the Brillouin frequency shift by the multi-taper appears as a spectral resonance in the baseband transfer function acquired by the BOFDA sensor. Any strain acting along the multi-taper affects the magnitude of this resonance, enabling dynamic strain measurements at that position. The fabrication of multi-taper arrays with different periods allows for the realization of more dynamic sensing positions along the same fiber. Measurements were performed on two vibrating cantilevers using two multi-taper arrays with lengths of 72 mm and 75 mm, respectively, at a sampling rate of 46 Hz.
Proceedings Paper
Engineering, Electrical & Electronic
Gianluca Persichetti, Genni Testa, Romeo Bernini, Emanuela Viaggiu, Roberta Congestri, Victor Dumas, Laurent Labbe
SENSORS AND MICROSYSTEMS, AISEM 2019
(2020)
