Journal
BIOMEDICAL OPTICS EXPRESS
Volume 4, Issue 2, Pages 187-192Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.4.000187
Keywords
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Funding
- Ministry of Knowledge Economy [10035559]
- National Research Foundation of Korea
- Ministry of Education, Science and Technology [2010-0014728]
- Korea Evaluation Institute of Industrial Technology (KEIT) [10035559] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2010-0014728] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In this study, we propose a new detection method of nanoparticle-enhanced human papillomavirus genotyping microarrays using a DVD optical pick-up with a photodiode. The HPV genotyping DNA chip was labeled using Au/Ag core-shell nanoparticles, prepared on a treatment glass substrate. Then, the bio information of the HPV genotyping target DNA was detected by measuring the difference of the optical signals between the DNA spots and the background parts for cervical cancer diagnosis. Moreover the approximate linear relationship between the concentration of the HPV genotyping target DNA and the optical signal depending on the density of Au/Ag core-shell nanoparticles was obtained by performing a spot finding algorithm. It is shown that the nanoparticle-labeled HPV genotyping target DNA can be measured and quantified by collecting the low-cost photodiode signal on the treatment glass chip, replacing high-cost fluorescence microarray scanners using a photomultiplier tube. (c) 2012 Optical Society of America
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