Review
Neurosciences
Xiaofan Jiang, Omar A. Mahroo
Summary: Research on the time taken for regaining visual sensitivity (dark adaptation) following bleaching exposures has found that it takes 30 minutes for rod-driven vision to recover, while cone-driven vision recovers within 100 milliseconds. These findings provide insights into the physiological processes underlying dark adaptation and may have clinical implications in the future.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Ophthalmology
Rigmor C. Baraas, Hilde R. Pedersen, Kenneth Knoblauch, Stuart J. Gilson
Summary: This study characterizes the association between foveal shape and cone and retinal pigment epithelium (RPE) cell topographies in healthy humans. The results show a strong relationship between cone and RPE cell spacing and the shape of the human fovea, indicating the critical role of RPE in foveal development.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Medicine, Research & Experimental
Bruce A. Berkowitz, Haohua Qian
Summary: The article reviews high spatial resolution imaging techniques and commercial OCT technologies for measuring mitochondrial respiration in the outer retina, and discusses the importance of changes in subretinal space water content for diagnosing threats to vision and improving treatment success rates.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Multidisciplinary Sciences
Hassan Rashidi, Yeh Chwan Leong, Kerrie Venner, Hema Pramod, Qi-Zhen Fei, Owen J. R. Jones, Dale Moulding, Jane C. Sowden
Summary: Retinal degenerative diseases are a major cause of blindness worldwide, but cell therapy shows promise in restoring and preserving sight. Researchers have successfully developed a large-scale retinal differentiation platform without the need for manual microdissection, and have generated retinal tissue using a stepwise differentiation protocol with small molecules.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Heike Kroeger, Julia M. D. Grandjean, Wei-Chieh Jerry Chiang, Daphne D. Bindels, Rebecca Mastey, Jennifer Okalova, Amanda Nguyen, Evan T. Powers, Jeffery W. Kelly, Neil J. Grimsey, Michel Michaelides, Joseph Carroll, R. Luke Wiseman, Jonathan H. Lin
Summary: ER stress and UPR signaling contribute to human diseases, including severe congenital vision loss conditions like achromatopsia. Loss-of-function ATF6 variants lead to defective cone formation in the retina, highlighting the essential role of ATF6 in human cone development. Pharmacologic targeting of the ATF6 pathway may hold potential for treating blinding retinal diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Vimal Prabhu Pandiyan, Phuong T. Nguyen, Edward N. Pugh, Ramkumar Sabesan
Summary: Using phase-resolved optical coherence tomography, this study measured the changes in length of human cone outer segments in response to bleaching stimuli. The results showed that these changes consist of a fast phase and two slower phases, with the slower phases being linearly related to cone pigment bleaching. The study also proposed hypotheses about the underlying mechanism of these changes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Optics
Somayyeh Soltanian-Zadeh, Kazuhiro Kurokawa, Zhuolin Liu, Furu Zhang, Osamah Saeedi, Daniel X. Hammer, Donald T. Miller, Sina Farsiu
Summary: Cell-level quantitative features of retinal ganglion cells (GCs) are important biomarkers for neurodegenerative diseases, with adaptive optics OCT enabling in vivo imaging. Weakly supervised deep learning-based automated segmentation of GC layer somas from AO-OCT volumes is as good as or even better than human experts, showing increased structure-function correlation in glaucoma subjects.
Article
Environmental Sciences
Artur Lenczuk, Anna Klos, Janusz Bogusz
Summary: This study uses probabilistic Principal Component Analysis (pPCA) to analyze the spatio-temporal variations of vertical displacements from 98 GPS stations to investigate the impact of groundwater mass changes on crustal displacements. The results show that the trend of vertical displacements induced by groundwater mass changes is overestimated by GRACE-WGHM data but underestimated by GPS-WGHM data. There are significant spatio-temporal patterns and correlations between GPS-WGHM displacements and GRACE-WGHM displacements with periods of intense groundwater mass changes. This study demonstrates that GPS displacements can provide essential information for studying and monitoring local displacements caused by groundwater mass changes.
REMOTE SENSING OF ENVIRONMENT
(2023)
Correction
Cell & Tissue Engineering
Aindrila Saha, Elizabeth Capowski, Maria A. Fernandez Zepeda, Emma C. Nelson, David M. Gamm, Raunak Sinha
Summary: ROs derived from human pluripotent stem cells demonstrate robust and wavelength-specific light-evoked responses similar to adult foveal cones. The photoresponses and membrane physiology of these lab-generated cones are comparable to intact ex vivo primate fovea. These results greatly enhance the potential of ROs as a source of functional human cones for cell replacement therapies, drug testing, and in vitro models of retinal dystrophies.
Article
Multidisciplinary Sciences
Manuela Voelkner, Felix Wagner, Lisa Maria Steinheuer, Madalena Carido, Thomas Kurth, Ali Yazbeck, Jana Schor, Stephanie Wieneke, Lynn J. A. Ebner, Claudia Del Toro Runzer, David Taborsky, Katja Zoschke, Marlen Vogt, Sebastian Canzler, Andreas Hermann, Shahryar Khattak, Joerg Hackermueller, Mike O. Karl
Summary: Human organoids can provide valuable insights into complex and incurable neuropathologies. In this study, researchers successfully established a human retinal organoid system that replicates various aspects of the human retina, including those within the macula. By combining TNF and HBEGF, the researchers induced the degeneration of photoreceptors, glial pathologies, dyslamination, and scar formation, uncovering a previously unknown pathomechanism. This research could have significant implications for age-related macular degeneration and other related diseases, offering potential therapeutic approaches.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Slawomir Tomczewski, Piotr Wegrzyn, Dawid Borycki, Egidijus Auksorius, Maciej Wojtkowski, Andrea Curatolo
Summary: Electroretinography (ERG) has long been used for studying retinal physiological function, and recently a new technique called optoretinography (ORG) has been developed using phase-sensitive optical coherence tomography (OCT) to measure the physiological response of retinal photoreceptors to visible light. In this study, the researchers used a spatio-temporal optical coherence tomography (STOC-T) system to capture optoretinograms with a flickering stimulus and demonstrated the ability to detect differences in the photoreceptor optical path length modulation amplitudes in response to different flicker frequencies. They also showed the potential of spatially mapping the retinal response to a patterned stimulus with ORG, allowing for characterization of the spatially-resolved temporal-frequency response of the retina.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Jeffrey M. Mcbride, Michael J. Hackmann, Sophia Nimphius, Barry Cense
Summary: This study investigates the feasibility of using a polarizatio-sensitive optical coherence tomography (PS-OCT) needle probe to accurately identify anatomical structures in human skeletal muscle. The results suggest that the PS-OCT needle probe can be inserted into live human skeletal muscle and identify relevant anatomical structures, potentially serving as a diagnostic tool for skeletal muscle pathology.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemistry & Molecular Biology
Xiaotian Jiang, Rabab Rashwan, Valentina Voigt, Jeanne Nerbonne, David M. Hunt, Livia S. Carvalho
Summary: Loss of Kv8.2 subunits in murine retina contributes to early cellular and physiological changes leading to retinal dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Guangying Ma, Taeyoon Son, Tae-Hoon Kim, Xincheng Yao
Summary: The study demonstrated optical coherence tomography (OCT) of amplitude-IOS and phase-IOS changes in human photoreceptors, showing rapid changes predominantly within the photoreceptor outer segment (OS) associated with phototransduction activation.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Ophthalmology
Markku Kilpelainen, Nicole M. Putnam, Kavitha Ratnam, Austin Roorda
Summary: Due to the significant difference in spatial resolution between the central fovea and the surrounding retinal regions, accurate fixation on important objects is critical for humans. Research found that healthy human observers consistently use the same preferred retinal location (PRL) across days and can accurately judge stimulus locations based on visual scene coordinates and retinal locations.
Article
Biochemical Research Methods
Sylvia Desissaire, Florian Schwarzhans, Matthias Salas, Andreas Wartak, Georg Fischer, Clemens Vass, Michael Pircher, Christoph K. Hitzenberger
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Biochemical Research Methods
Muhammad Faizan Shirazi, Elisabeth Brunner, Marie Laslandes, Andreas Pollreisz, Christoph K. Hitzenberger, Michael Pircher
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Biochemical Research Methods
Sylvia Desissaire, Andreas Pollreisz, Aleksandra Sedova, Dorottya Hajdu, Felix Datlinger, Stefan Steiner, Clemens Vass, Florian Schwarzhans, Georg Fischer, Michael Pircher, Ursula Schmidt-Erfurth, Christoph K. Hitzenberger
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Biochemical Research Methods
Florian Schwarzhans, Sylvia Desissaire, Stefan Steiner, Michael Pircher, Christoph K. Hitzenberger, Hemma Resch, Clemens Vass, Georg Fischer
BIOMEDICAL OPTICS EXPRESS
(2020)
Article
Biochemical Research Methods
Elisabeth Brunner, Julia Shatokhina, Muhammad Faizan Shirazi, Wolfgang Drexler, Rainer Leitgeb, Andreas Pollreisz, Christoph K. Hitzenberger, Ronny Ramlau, Michael Pircher
Summary: The potential advantages of P-WFS in ophthalmic imaging have not been fully realized, but a cost-effective AO correction method has been proposed in this study, which has been successfully applied to human retinal imaging.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Biochemical Research Methods
Alice R. Motschi, Philipp K. Roberts, Sylvia Desissaire, Markus Schranz, Florian Schwarzhans, Hrvoje Bogunovic, Michael Pircher, Christoph K. Hitzenberger
Summary: A new algorithm for automatic detection, segmentation, and quantification of fibrotic lesions in 3D data sets recorded by PS-OCT was presented in this study. The algorithm showed high precision in detecting fibrosis and agreement with clinical diagnosis, particularly in cases of smaller lesion areas.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Biochemical Research Methods
Sylvia Desissaire, Florian Schwarzhans, Stefan Steiner, Clemens Vass, Georg Fischer, Michael Pircher, Christoph K. Hitzenberger
Summary: By utilizing TPE-OCT methods, we have successfully evaluated retinal tissue deformation in vivo over time periods of several seconds, demonstrating precision and repeatability of measurements. A reproducible phase pattern is observed at each heartbeat cycle for all imaged volunteers, with differences in motion induced by arteries and veins also investigated.
BIOMEDICAL OPTICS EXPRESS
(2021)
Editorial Material
Biochemical Research Methods
Christoph K. Hitzenberger, Adam Gibson
Summary: Biomedical Optics Express Editors-in-Chief Christoph K. Hitzenberger and Deputy Editor Adam Gibson shared final remarks as they approach the end of their editorial terms.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Florian Schwarzhans, Sylvia Desissaire, Stefan Steiner, Michael Pircher, Christoph K. Hitzenberger, Hemma Resch, Clemens Vass, Georg Fischer
Summary: This paper describes a technique to accurately estimate the trajectories of retinal nerve fiber bundles (RNFB) in a large field of view image. The method utilizes stitched projections of polarization-sensitive optical coherence tomography (PS-OCT) data and a mathematical model of average RNFB trajectories as prior knowledge. The fully automatic process was applied to data recorded in healthy subjects and glaucoma patients, and compared to manual traces.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Alice R. Motschi, Florian Schwarzhans, Sylvia Desissaire, Stefan Steiner, Hrvoje Bogunovic, Philipp K. Roberts, Clemens Vass, Christoph K. Hitzenberger, Michael Pircher
Summary: Conventional OCT has difficulty imaging Henle fibers (HF) due to their low backscattering potential. However, polarization-sensitive (PS) OCT can visualize HF by exploiting the form birefringence of fibrous structures. We developed a new measure using PS-OCT to estimate the presence of HF at various eccentricities from the fovea and found that glaucoma patients showed a slightly decreased retardation at 2° to 7.5° from the fovea, indicating potential early effects of glaucoma on this neuronal tissue.
BIOMEDICAL OPTICS EXPRESS
(2023)
