4.6 Article

Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy

Journal

BIOMEDICAL OPTICS EXPRESS
Volume 1, Issue 2, Pages 706-719

Publisher

OPTICAL SOC AMER
DOI: 10.1364/BOE.1.000706

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Funding

  1. National Science Foundation (NSF) [CBET-0651622]
  2. Bikura Postdoctoral Fellowship from Israel
  3. Div Of Chem, Bioeng, Env, & Transp Sys
  4. Directorate For Engineering [1039562] Funding Source: National Science Foundation

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We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution. The whole-cell phase profiles are analyzed to yield valuable quantitative parameters characterizing the cell dynamics, without the need to decouple thickness from refractive index differences. Our experimental results verify that these new parameters can be used with wide field interferometric microscopy to discriminate the modulation of cardiomyocyte contraction dynamics due to temperature variation. To demonstrate the necessity of the proposed numerical analysis for cardiomyocytes, we present confocal dual-channel fluorescence microscopy results which show that the rapid motion of the cell organelles during contraction preclude assuming a homogenous refractive index over the entire cell contents, or using multiple-exposure or scanning microscopy. (C) 2010 Optical Society of America

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