Article
Biotechnology & Applied Microbiology
Yu-An Sui, Shigenobu Kishino, Satoshi Maruyama, Masakazu Ito, Masayoshi Muramatsu, Shusei Obata, Jun Ogawa
Summary: The study discovered that the alcohol dehydrogenase PsADH from Pantoea sp. 7-4 can oxidize 1-tetradecanol to tetradecanal, providing a new pathway for direct alkane production from alcohol.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu-An Sui, Satoshi Maruyama, Natsumi Okada, Masakazu Ito, Masayoshi Muramatsu, Shusei Obata, Jun Ogawa, Shigenobu Kishino
Summary: PsADH, an alcohol dehydrogenase from Pantoea sp., was found to convert a variety of fatty alcohols into their corresponding aldehydes for alkane biosynthesis. By coupling PsADH with NpAD, a cyanobacterial aldehyde-deformylating oxygenase, and optimizing the reaction conditions, a 52% conversion of 1-tetradecanol to tridecane was achieved. This system was further used to generate alkanes ranging from C5-17, which can serve as biofuels, demonstrating the effectiveness of introducing a suitable alcohol dehydrogenase for alkane production.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2023)
Article
Chemistry, Physical
Wenjin Li, Yanxin Gao, Xuan Sun, Lei Wan, Haishuo Ji, Hang Luo, Yao Tian, Hao Song, Geng Wu, Liyun Zhang
Summary: In this study, the adsorption characteristics and catalytic properties of formate dehydrogenase from Clostridium ljungdahlii (ClFDH) on electrodes were investigated. The electrochemical signals observed on the electrodes were attributed to the reduction and oxidation potential of the [4Fe-4S](2+/+) cluster. Upon adding substrates, the signals transformed into a specific redox center that catalytically engages in electron transport and promotes the rapid and efficient reversible interconversion between CO2 and formate. The results provide valuable insights into the understanding of the catalytic mechanism of FDH and the design of synthetic catalysts.
Article
Chemistry, Applied
Marc Biermann, Martin Schuermann, Thomas Schmitges, Andreas Vogel, Jan Brummund
Summary: A biotransformation method to produce (R)-Undecavertol has been developed and successfully demonstrated at the pilot scale with high productivity. By using a highly selective alcohol dehydrogenase and NAD(P)H oxidase, racemic resolution of the substrate is achieved, and oxygen limitation is prevented by the supply of pure oxygen.
ORGANIC PROCESS RESEARCH & DEVELOPMENT
(2022)
Review
Microbiology
Shoshana C. Williams, Rachel Narehood Austin
Summary: This Mini Review provides an overview of the known canonical arrangement of electron-transfer proteins that activate AlkB and explores other possible arrangements. The diversity of these arrangements has biological significance and raises key questions for future research.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shoshana C. Williams, Allison P. Forsberg, Juliet Lee, Christina L. Vizcarra, Allison J. Lopatkin, Rachel N. Austin
Summary: The research identified over 100 distinct alkane monooxygenase (AlkB) enzymes containing a covalently bound, or fused, rubredoxin. Mutations introduced into the enzymes affected their activity and substrate selectivity.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Sunney I. Chan, Phimonphan Chuankhayan, Pavan Kumar Reddy Nareddy, I-Kuen Tsai, Yi-Fang Tsai, Kelvin H. -C. Chen, Steve S. -F. Yu, Chun-Jung Chen
Summary: The active site of methanol dehydrogenase contains a rare disulfide bridge that may work together with the PQQ prosthetic group and Ca2+ ion during catalysis. Studies show that the disulfide bridge undergoes redox reactions within the protein, producing a disulfide radical anion and a companion PQQ radical. These findings suggest a mechanism for controlled electron redistribution during alcohol oxidation, avoiding the formation of reduced PQQ ethenediol and allowing for possible recovery of the hydride for multiple catalytic turnovers.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Sharad Sarak, Taresh P. Khobragade, Hyunwoo Jeon, Amol D. Pagar, Pritam Giri, Somin Lee, Hyungdon Yun
Summary: A multienzyme biocatalytic cascade was developed to synthesize nylon monomers with high efficiency and a self-sufficient redox network, utilizing a cell-based modularization strategy to produce 6-aminohexanoic acid and epsilon-caprolactam from cyclohexanol. The designed E. coli consortia showed high yields of the desired products, making it a cost-effective method for nylon precursor production.
Article
Chemistry, Physical
Taiki Adachi, Tomoko Miyata, Fumiaki Makino, Hideaki Tanaka, Keiichi Namba, Kenji Kano, Keisei Sowa, Yuki Kitazumi, Osamu Shirai
Summary: A mediatorless bioanode for ethanol oxidation was constructed based on a direct electron transfer (DET)-type bienzymatic cascade, which achieved efficient conversion of biomass fuels.
Article
Materials Science, Paper & Wood
Paula M. R. Higasi, Igor Polikarpov
Summary: Enzymatic deconstruction of cellulose has been revolutionized by the discovery of Lytic Polysaccharide Monooxygenases (LPMOs). These copper-dependent enzymes break the cellulose chain through oxidative processes, facilitating cellulose degradation. In this study, the LPMO TtLPMO9H showed activity towards cellulose when an aryl-alcohol oxidase (TtAAOx) and its substrate veratryl alcohol were present. The production and release of oxidized oligosaccharides by TtLPMO9H required the presence of methoxylated benzyl alcohols and either TtAAOx for in situ production or an exogenous supply of H2O2.
Article
Materials Science, Paper & Wood
Paula M. R. Higasi, Igor Polikarpov
Summary: The discovery of Lytic Polysaccharide Monooxygenases (LPMOs) has revolutionized the enzymatic deconstruction of cellulose. These copper-dependent enzymes break the cellulose chain through an oxidative process, facilitating deconstruction by cellulases. The activity of the LPMO enzyme towards cellulosic substrate is dependent on the presence of an aryl-alcohol oxidase (TtAAOx) and its substrate, veratryl alcohol. Additionally, the presence of methoxylated benzyl alcohols and either TtAAOx or an exogenous supply of H2O2 is required for the formation and release of oxidized oligosaccharides by the LPMO.
Article
Energy & Fuels
Priyanki Das, Vinay Bachu, Lepakshi Barbora, Arup Dutta, Mrinal Kumar Sarma, Pranab Goswami
Summary: This study presents critical findings on a methanol-fueled enzymatic biofuel cell using alcohol oxidase and bilirubin oxidase as catalysts, demonstrating high operational stability through efficient design improvements.
Article
Biochemistry & Molecular Biology
Bipasa Dey, Tanmay Dutta
Summary: This review provides a comprehensive overview of laccase-driven electrocatalytic reactions, including the structural and functional features of laccases that contribute to the electron transfer mechanism and reduction potential. It explores the application prospects of tailored laccase in various fields and emphasizes the importance of enzyme engineering in the design of optimal bioelectrocatalysts.
BIOELECTROCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sharad Sarak, Amol D. Pagar, Taresh P. Khobragade, Hyunwoo Jeon, Pritam Giri, Seonga Lim, Mahesh D. Patil, Yechan Kim, Byung-Gee Kim, Hyungdon Yun
Summary: A multienzymatic cascade for the synthesis of 1,6-hexamethylenediamine (HMD) and related alpha,omega-diamines from corresponding cycloalkanols was developed. The biosynthetic pathway was distributed into different modules using an E. coli-based modularization strategy. An engineered transaminase variant was used to drive the reaction towards target product formation, resulting in a high yield of HMD.
Article
Thermodynamics
Halil Yalcin Akdeniz, Ozgur Balli
Summary: In this paper, an extensive assessment of a turbojet engine powered by biofuel, hydrogen, and JP-8 fuels is carried out based on thermodynamic principles. Results show varying fuel mass stream, exhaust gaseous mass stream rate, kinetic energy rate, energy efficiency, specific fuel consumption, and exergetic efficiency when using biofuel and hydrogen as compared to JP-8 fuel utilization.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2021)
Article
Chemistry, Multidisciplinary
Olja Simoska, Zayn Rhodes, Emily Carroll, Katia N. Petrosky, Shelley D. Minteer
Summary: A Redox flow battery (RFB) utilizing genetically engineered Escherichia coli to regenerate degraded redox-active phenazines was developed. This novel approach improves the stability of RFB systems by preventing degradation of anolyte species after battery cycling.
CHEMICAL COMMUNICATIONS
(2023)
Review
Chemistry, Multidisciplinary
Bo Wang, Yifeng Zhang, Shelley D. D. Minteer
Summary: Ammonia is crucial for the fertilizer industry and the global chemical economy. However, the traditional Haber-Bosch process for ammonia synthesis is energy and capital-intensive, as well as associated with high greenhouse gas emissions. Therefore, green ammonia synthesis has become a research hotspot. Among various approaches, nature-inspired bioelectrocatalytic nitrogen fixation (e-BNF) is emerging as a cutting-edge, carbon-neutral, energy-efficient, and potentially sustainable strategy. However, the development of e-BNF is still in its early stages.
ENERGY & ENVIRONMENTAL SCIENCE
(2023)
Editorial Material
Chemistry, Organic
Kevin Lam, Shelley D. Minteer, Darren L. Poole
Summary: Guest editors Kevin Lam, Shelley D. Minteer, and Darren L. Poole introduce the themed issue on synthetic electrochemistry in Organic & Biomolecular Chemistry.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Physical
N. Samali Weliwatte, Hui Chen, Tianhua Tang, Shelley D. Minteer
Summary: By combining bioelectrocatalytic multienzyme cascade with organocatalysis, we have effectively addressed the challenges of selectively activating chemically inert C-H bonds and systematically functionalizing compounds. This design allows for high control over the substrate and enables more complex functionalization, making it versatile and adaptable.
Article
Electrochemistry
Kevin Beaver, Ashwini Dantanarayana, Ana Bonizol Zani, Danielle L. Lehto, Shelley D. Minteer
Summary: Microbial electrochemical systems have wide applications in bioremediation, biosensing, and bioenergy. This study evaluated the effects of biofilm on metabolic current output in mediated electron transfer systems and found that nitric oxide treatment had varying effects on different bacterial species. The results also showed that biofilm-based mediated electron transfer systems benefit from increased electrochemical output and enhanced cell adhesion.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Editorial Material
Chemistry, Organic
Shelley Minteer, Jingguang Chen, Song Lin, Cathleen Crudden, Stefanie Dehnen, Prashant V. Kamat, Marisa Kozlowski, Geraldine Masson, Scott J. Miller
Article
Chemistry, Analytical
Marcela Herrera-Dominguez, Koun Lim, Iris Aguilar-Hernandez, Alejandra Garcia-Garcia, Shelley D. Minteer, Nancy Ornelas-Soto, Raul Garcia-Morales
Summary: A bioelectrode using immobilized enzyme on carbon paper electrode modified with MoS2 was developed for the detection of acetaminophen. The best detection was achieved with a concentration of 1 mg/mL of MoS2. The laccase enzyme LacII showed the best biosensing efficiency with an LOD of 0.2 mu M and a sensitivity of 0.108 mu A/mu M cm(2) in the buffer matrix.
Editorial Material
Chemistry, Physical
Shelley Minteer, Jingguang Chen, Song Lin, Cathleen Crudden, Stefanie Dehnen, Prashant Kamat, Marisa Kozlowski, Geraldine Masson, Scott J. Miller
Editorial Material
Chemistry, Physical
Shelley Minteer, Jingguang Chen, Song Lin, Cathleen Crudden, Stefanie Dehnen, Prashant V. Kamat, Marisa Kozlowski, Geraldine Masson
ACS ENERGY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Isaac P. A. Guynn, Kevin Beaver, Erin M. Gaffney, Ana Bonizol Zani, Ashwini Dantanarayana, Shelley D. Minteer
Summary: S. EAGSL is a halotolerant and electroactive bacterium isolated from the Great Salt Lake. It has the ability to bridge the gap between power output and halotolerance in microbial fuel cells. The biosynthetic gene for ectoine, a valuable chemical with protein-stabilizing properties, has been identified in S. EAGSL.
CELL REPORTS PHYSICAL SCIENCE
(2023)
Editorial Material
Chemistry, Analytical
Shelley Minteer, Jingguang Chen, Song Lin, Cathleen Crudden, Stefanie Dehnen, Prashant V. Kamat, Marisa Kozlowski, Geraldine Masson, Scott J. Miller
ACS MEASUREMENT SCIENCE AU
(2023)
Editorial Material
Chemistry, Multidisciplinary
Shelley Minteer, Jingguang Chen, Song Lin, Cathleen Crudden, Stefanie Dehnen, Prashant V. Kamat, Marisa Kozlowski, Geraldine Masson, Scott J. Miller
ACS ORGANIC & INORGANIC AU
(2023)
Article
Chemistry, Multidisciplinary
Jian Luo, Michael. T. T. Davenport, Chad Callister, Shelley. D. D. Minteer, Daniel. H. H. Ess, T. Leo Liu
Summary: In this study, the mechanism of Ni-catalyzed electrochemical aryl amination reactions was investigated through experiments and calculations. The results revealed that the coordination of an amine to the Ni-II catalyst, followed by the generation of a stable Ni-II aryl amido intermediate and reductive elimination, were key steps in the reaction. The findings provide new insights into the mechanism of this reaction and offer guidance for the development of other Ni-catalyzed electrosynthetic reactions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Olja Simoska, Dale A. Cummings, Erin M. Gaffney, Claire Langue, Tommy G. Primo, Courtney J. Weber, Corbin E. Witt, Shelley D. Minteer
Summary: Metabolic engineering of E. coli to produce phenazine electron mediator carriers is an efficient and promising strategy for establishing efficient electron transfer in bioelectrochemical systems and enhancing the performance of microbial fuel cells.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Chemistry, Analytical
Jiahe Xu, Miharu Koh, Shelley D. Minteer, Carol Korzeniewski
Summary: This paper describes a spectroelectrochemical cell for confocal Raman microscopy studies of electrode-supported films. By optimizing spectral subtraction technique, potential-induced changes in the chemical composition of microscale redox films on carbon disk electrodes can be measured. The experiments demonstrate the effectiveness of this method in detecting transformations of redox-active molecules in ionomer membranes supported by electrodes.
ACS MEASUREMENT SCIENCE AU
(2023)
