Journal
ONCOLOGY LETTERS
Volume 7, Issue 5, Pages 1689-1694Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2014.1931
Keywords
notch1; microRNA-34a; renal cell carcinoma; target oncogenes; cell proliferation; cell cycle
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Funding
- National Natural Science Foundation of China [81372753]
- Science and Technology Commission of Shanghai Municipality [124119a2200]
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MicroRNA-34a (miR-34a) is a tumor suppressive microRNA, which induces G1 arrest, apoptosis and senescence by repressing the expression of multiple oncogenes. This study aimed to investigate the biological function and molecular mechanisms of miR-34a in human renal cell carcinoma (RCC) cells. Quantitative polymerase chain reaction (qPCR) revealed that miR-34a expression was significantly downregulated in eight of the 10 (80%) RCC tissues compared with adjacent normal tissues. In RCC cell lines, several other target genes of miR-34a were dysregulated at the mRNA level when the expression of miR-34a was elevated. In addition, western blot analysis and qPCR revealed that forced expression of miR-34a downregulated the expression of Notch1 at the protein and mRNA level. The Cell Counting Kit-8 identified that transient forced expression of miR-34a inhibited cell growth and resulted in cell cycle arrest, which was evaluated by flow cytometry. Our data demonstrated that miR-34a inhibits cell proliferation by downregulating Notch1 in RCC cell lines.
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