Angiopoietin-like protein ANGPTL2 gene expression is correlated with lymph node metastasis in lung cancer

Inflammation plays key roles at various stages of tumor development, including invasion and metastasis. In mice, the angiopoietin-like protein (ANGPTL2) gene has been implicated in inflammatory carcinogenesis. ANGPTL2 mRNA expression was investigated by real-time polymerase chain reaction (RT-PCR) assay using LightCycler in surgically treated non-small cell lung cancer (NSCLC) cases. In total, 110 surgically resected NSCLC cases were used for mRNA level analyses. The ANGPTL2/beta-actin mRNA levels were not significantly different between lung cancer (1598.481 +/- 6465.781) and adjacent normal lung tissues (2116.639 +/- 8337.331, P=0.5453). The tumor/normal (T/N) ratio of ANGPTL2/beta-actin mRNA levels was not different between gender, age, smoking status and pathological stages. The T/N ratio of ANGPTL2/beta-actin mRNA levels was significantly higher in lymph node metastasis-positive cases (2.173 +/- 3.151) compared with lymph node metastasis-negative cases (1.212 +/- 1.778, P=0.0464). However, ANGPTL2 mRNA status was not correlated with tumor invasion status. Thus, ANGPTL2 may drive metastasis and provide a candidate for blockade of its function as a strategy to antagonize the metastatic process in NSCLC.