Journal
JOURNAL OF PLANT INTERACTIONS
Volume 9, Issue 1, Pages 275-284Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/17429145.2013.824622
Keywords
Rhizobium tibeticum; isoflavonoids; salinity; beta-galactosidase; nodulation
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Funding
- Science and Technology Development Fund (STDF), Ministry of Higher Education and Scientific Research, Egypt [12]
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Rhizobia-legume symbiosis depends on molecular dialog, which involves the production of specific plant flavonoid compounds as signal molecules. Rhizobium tibeticum was recovered from the root nodule of fenugreek and identified by sequencing the 16S rRNA gene. The effect of salinity stress on nod gene expression was measured in terms of beta-galactosidase activity. R. tibeticum containing Escherichia coli lacZ gene fusions to specific nodulation (nod) genes were used to determine beta-galactosidase activity. Combination of hesperetin (7.5 mu M) and apigenin (7.5 mu M) significantly increased beta-galactosidase activity more than the single application of hesperetin or apigenin. Preincubation of R. tibeticum with hesperetin and apigenin combination significantly alleviates the adverse effect of salinity on nod gene expression and therefore, enhances nodulation and nitrogen fixation of fenugreek.
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