Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 8, Issue 5, Pages 1389-1396Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2014.1985
Keywords
umbilical cord; Wharton's jelly; mesenchymal stem cells; transplantation; diabetes mellitus
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Funding
- Beijing Municipal Administration of Traditional Chinese Medicine [WZF2012-13, WZF2012-02]
- Beijing Municipal Health Bureau Talent Project [2012-18]
- Beijing Municipal Health System High-level Health Technicians Project [2013-3-080]
- Beijing Science and Technology Committee Capital Special Project [Z121107001012103]
- Beijing Traditional Chinese Medicine Technology Development Fund [JJ2013-03]
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The aim of the present study was to investigate the roles of the co-culture of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hUC-MSCs) with rat pancreatic cells in the treatment of rats with diabetes hUC-MSCs were isolated and passaged, followed by Transwell co-culture with rat pancreatic cells. The induced islet-like cell clusters were transplanted into the renal capsule in rats with streptozotocin (STZ)-induced diabetes mellitus. The effects of co-culture on blood glucose levels in rats were observed. The isolated hUC-MSCs expressed the specific surface markers, including cluster of differentiation 44 (CD44) (91.4%), CD29 (91.3%) and CD105 (99.2%). Following co-culture with hUC-MSCs for 7 and 10 days, the rat pancreatic cells were strongly stained by pancreatic and duodenal homeobox-1 and human insulin. The insulin and C-peptide concentrations were increased significantly compared to the pure culture group. One week following the transplantation of induced islet-like cells into the renal capsule, the blood glucose level of rats in the STZ experimental group was significantly lower than that of the STZ control group. There were notable 5-bromo-2'-deoxyuridine-positive nuclei and insulin-positive cytoplasm in the renal capsule following cell transplantation. Therefore, co-culture of hUC-MSCs with rat pancreatic cells can lower the blood glucose levels in rats with diabetes mellitus.
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