Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 7, Issue 1, Pages 246-250Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2013.1373
Keywords
Tagetes erecta L; elastase inhibitor; tyrosinase inhibitor; H460 cells; Caco-2 cells; marigold flower extracts
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Funding
- Thai Herbal Nano-Cosmeceuticals Coordinated Research Program [P-00-10647]
- National Nanotechnology Center
- National Science and Technology Development Agency, Thailand
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Marigold (Tagetes erecta L.) has long been used as a medicinal herb for a number of therapeutic activities. In the present study, the cytotoxicities of ethanol and ethyl acetate extracts of marigold flowers and their inhibitory effects on elastase and tyrosinase enzymes were investigated. An MTT assay was performed to measure the cytotoxicity of these two extracts on the H460 lung cancer and the Caco-2 colon cancer cell lines. An elastase assay kit, based on the digestion of a non-fluorescent elastin substrate to highly fluorescent fragments by elastase, was used for the elastase inhibition assay. Tyrosinase inhibition activity was investigated using the dopachrome method with L-3,4-dihydroxyphenylalanine (L-DOPA) as a substrate. The data obtained in this study demonstrated that the extracts were nontoxic to H460 and Caco-2 cell lines. The elastase inhibition activities of ethanol (250 g/ml) and ethyl acetate (125 g/ml) extracts were found to be significantly higher than that of the negative control. The tyrosinase inhibition activities of ethanol and ethyl acetate extracts, in terms of the mean inhibition concentration (IC50), were 1,078 and 1,467 g/ml, respectively. To the best of our knowledge, the present study has demonstrated for the first time that marigold flower extracts possess tyrosinase inhibition activity. The activities of ethanol and ethyl acetate extracts of marigold flowers were investigated in vitro and indicated that these extracts possess useful properties that may be of interest for cosmetic development.
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