Article
Endocrinology & Metabolism
Huan Liu, Shirin Geravandi, Ausilia Maria Grasso, Saheri Sikdar, Alberto Pugliese, Kathrin Maedler
Summary: This study found no association between enteroviral infection in the pancreas and T2D diabetes. Despite similarities in inflammatory markers in islets in both T1D and T2D, long-term enteroviral infiltration is a distinct pathological feature of T1D-associated autoimmunity and T1D pancreases.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell Biology
Yasir Mohamud, Hui Tang, Yuan Chao Xue, Huitao Liu, Chen Seng Ng, Amirhossein Bahreyni, Honglin Luo
Summary: Coxsackievirus B3 targets TFEB for proteolytic processing to disrupt host lysosomal function and enhance viral infection.
Article
Virology
Yiyun Michelle Fan, Yizhuo Lyanne Zhang, Amirhossein Bahreyni, Honglin Luo, Yasir Mohamud
Summary: This study investigated how enteroviruses utilize the cellular autophagy pathway for replication. The authors discovered that Coxsackievirus B3 (CVB3) selectively degrades the autophagy cysteine protease ATG4A and identified the site at which CVB3 protease 2A cleaves ATG4A. Furthermore, cleavage of ATG4A was found to result in a loss of autophagy function.
Article
Cell Biology
Mehrdad Alirezaei, Claudia T. Flynn, Selma D. Garcia, Taishi Kimura, J. Lindsay Whitton
Summary: Food restriction in mice increases susceptibility to attenuated enterovirus infection, with TFEB playing a crucial role in regulating this effect. TFEB's rapid increase in expression and activation status under food restriction influences the host's susceptibility to coxsackievirus B3 infection.
Article
Virology
Anna Zimina, Ekaterina G. Viktorova, Seyedehmahsa Moghimi, Jules Nchoutmboube, George A. Belov
Summary: Through our study on poliovirus, we found that the capsid protein VP0 interacts with the cellular autophagy protein LC3A, while VP0 and P1 proteins strongly colocalize with the autophagy marker p62/SQSTM1. Furthermore, our research indicates that there is a specific autophagy response during infection, with the capsid proteins possibly being targeted to p62/SQSTM1-dependent sequestration in order to control viral protein levels and prevent premature removal of viral RNAs from the replication/translation pool.
Review
Cell Biology
Ming Yang, Shilu Luo, Xi Wang, Chenrui Li, Jinfei Yang, Xuejing Zhu, Li Xiao, Lin Sun
Summary: The endoplasmic reticulum (ER) is crucial for maintaining cellular homeostasis, and ER-phagy, a selective form of autophagy, helps remove damaged ER to protect cells from harm caused by excessive ER stress. Many receptor-mediated ER-phagy pathways have been discovered in recent years, highlighting the importance of this newly identified autophagy process in maintaining cellular health.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Cell Biology
Yaiza R. Varela, Alicia Alonso
Summary: The interaction between LC3/GABARAP proteins and specific lipids plays a crucial role in their function. Cardiolipin and ceramide have been found to be essential for the protein-bilayer interaction.
Article
Virology
Florian Hinte, Jendrik Mueller, Wolfram Brune
Summary: The Unfolded Protein Response (UPR) and Endoplasmic Reticulum-Associated Degradation (ERAD) play crucial roles in protein quality control in the secretory pathway. Viruses like cytomegaloviruses modulate the UPR to benefit their replication by degrading IRE1, a sensor molecule for UPR. The viral M50 protein degrades IRE1 using the ERAD machinery, facilitating virus replication.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yaiza R. Varela, Marina N. Iriondo, Asier Etxaniz, Uxue Ballesteros, L. Ruth Montes, Felix M. Goni, Alicia Alonso
Summary: This study explores the interaction of LC3/GABARAP proteins with phospholipid monolayers and bilayers. It finds that cardiolipin is essential for the protein interaction with bilayers, while ceramide markedly increases binding. Confocal fluorescence microscopy experiments reveal the arrangement of ceramide in the phospholipid bilayers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biotechnology & Applied Microbiology
Ting Cao, Binfeng Peng, Xiangping Zhou, Jialun Cai, Yun Tang, Jie Luo, Haitao Xie, Ji Zhang, Shuangquan Liu
Summary: The endoplasmic reticulum (ER) is a crucial multifunctional organelle responsible for correct folding and assembly of secretory and transmembrane proteins. Perturbations in ER function can lead to ER stress, activating the unfolded protein response (UPR) to cope with misfolded protein accumulation. If the imbalance cannot be restored, it may lead to cell death or diseases. The UPR is a coordination system that regulates transcription and translation to help cells recover ER homeostasis. The integrated signaling system in cells includes ER-associated degradation (ERAD), autophagy, hypoxia signaling, and mitochondrial biogenesis to cope with ER stress.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Bin Wang, Jing Zhang, Xin Liu, Qingqing Chai, Xiaoran Lu, Xiaoyu Yao, Zhichang Yang, Liangliang Sun, Silas F. Johnson, Richard C. Schwartz, Yong-Hui Zheng
Summary: Zaire ebolavirus (EBOV) suppresses the expression of its structural glycoprotein (GP) through protein disulfide isomerases (PDIs), resulting in reduced pathogenicity. PDIs trigger misfolding of GP by targeting its cysteine residues and activate the unfolded protein response (UPR), leading to degradation of misfolded GP through ER-associated protein degradation (ERAD) and autophagy-lysosomal pathway. PDIs also have similar inhibitory activity on GP expression from other ebolavirus species.
Article
Biochemistry & Molecular Biology
Haruka Chino, Akinori Yamasaki, Koji L. Ode, Hiroki R. Ueda, Nobuo N. Noda, Noboru Mizushima
Summary: This study reveals the critical role of LIR phosphorylation in the interaction, localization, and initiation of autophagy of the ER-phagy receptor TEX264 with ATG8. Structural analysis shows that phosphorylation increases binding affinity by generating multiple hydrogen bonds with ATG8, which cannot be mimicked by acidic residues. This finding highlights the importance of LIR phosphorylation in LIR-ATG8 interactions.
Article
Biochemistry & Molecular Biology
Tokuya Hattori, Ken Hanafusa, Ikuo Wada, Nobuko Hosokawa
Summary: During ER-associated degradation (ERAD), degradation of SEL1L generates a ladder of degradation products, with the C-terminal Pro-rich region responsible for this pattern. These degradation intermediates in the cytosol interact with aggregation-prone proteins, promoting protein aggregation. The findings reveal the interconnection of protein homeostasis across subcellular compartments.
Article
Oncology
Ellen J. Tisdale, Cristina R. Artalejo
Summary: The Golgi complex is a dynamic organelle that regulates cellular activities and maintains a distinct structure. Rab2 is a protein involved in Golgi structure and its amplification is associated with human cancers. Rab2 overexpression affects Golgi morphology and transport, stimulates LC3-lipidation, and influences Golgi-associated signaling pathways that may contribute to oncogenesis.
EXPERIMENTAL CELL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Francisco Jose Martinez-Amaro, Carlos Garcia-Padilla, Diego Franco, Houria Daimi
Summary: The endoplasmic reticulum (ER) is crucial for protein quality control and maintaining protein homeostasis. ER stress triggers various molecular mechanisms such as ERAD, UPR, and reticulophagy to restore protein balance. Failure to alleviate ER stress leads to apoptosis and cardiovascular diseases. The roles of microRNAs, as well as lncRNAs and circRNAs, in modulating ER stress response and their impact on cardiovascular diseases are discussed in this review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)