Journal
PPAR RESEARCH
Volume 2009, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2009/369602
Keywords
-
Categories
Funding
- Canadian Institutes of Health Research (CIHR) [MOP-200609]
Ask authors/readers for more resources
Omega-3 fatty acids (FAs) have the potential to regulate gene expression via the peroxisome proliferator-activated receptor alpha (PPAR alpha); therefore, genetic variations in this gene may impact its transcriptional activity on target genes. It is hypothesized that the transcriptional activity by wild-type L162-PPAR alpha is enhanced to a greater extent than the mutated variant (V162-PPAR alpha) in the presence of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) or a mixture of EPA: DHA. To examine the functional difference of the two allelic variants on receptor activity, transient co-transfections were performed in human hepatoma HepG2 cells activated with EPA, DHA and EPA: DHA mixtures. Results indicate that the addition of EPA or DHA demonstrate potential to increase the transcriptional activity by PPAR alpha with respect to basal level in both variants. Yet, the EPA: DHA mixtures enhanced the transcriptional activity to a greater extent than individual FAs indicating possible additive effects of EPA and DHA. Additionally, the V162 allelic form of PPAR alpha demonstrated consistently lower transcriptional activation when incubated with EPA, DHA or EPA: DHA mixtures than, the wild-type variant. In conclusion, both allelic variants of the PPAR alpha L162V are activated by omega-3 FAs; however, the V162 allelic form displays a lower transcriptional activity than the wild-type variant. Copyright (C) 2009 Iwona Rudkowska et al.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available