Review
Cell & Tissue Engineering
Shaoxiong Yu, Saihua Yu, Haiyan Liu, Naishun Liao, Xiaolong Liu
Summary: This review summarizes the current status and challenges of mesenchymal stem cell (MSC) transplantation for end-stage liver disease (ESLD). It highlights the importance of improving cell engraftment efficiency in enhancing MSC therapy for liver diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Pierfrancesco Pagella, Cesar Nombela-Arrieta, Thimios A. Mitsiadis
Summary: Specific stem cell populations within dental mesenchymal tissues ensure tooth homeostasis and regeneration, with the decision between renewal and differentiation influenced by interactions with stromal cells and extracellular matrix molecules. The Cxcl12 chemokine, a general marker of stromal cells, plays crucial roles in stem cell maintenance, mobilization, and migration. Using Cxcl12-GFP transgenic mice, this study revealed widespread expression of Cxcl12 in intact and injured teeth, highlighting its significance in various dental mesenchymal stem cell niches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Pei Tian, Andrew Elefanty, Edouard G. Stanley, Jennifer C. Durnall, Lachlan H. Thompson, Ngaire J. Elwood
Summary: Clinical-grade induced pluripotent stem cells (iPSCs) can be efficiently created using small volumes of thawed cord blood from public cord blood banks. This study presents a protocol that involves ex-vivo expansion and reprogramming of erythroid progenitor cells derived from cord blood, resulting in iPSC lines that are tested for pluripotency, genomic integrity, and stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Sydney Felker, Archana Shrestha, Jeff Bailey, Devin M. Pillis, Dylan Siniard, Punam Malik
Summary: The study found that intra-bone marrow transplantation improved engraftment of hematopoietic progenitor cells but not long-term repopulating hematopoietic stem cells. Hematopoietic progenitor cells expressed higher levels of functional CXCR4 than hematopoietic stem cells, giving them an advantage in retention and homing during intra-bone marrow transplantation.
Article
Chemistry, Multidisciplinary
Yong Xie, Yi Sun, Yudong Liu, Jinyun Zhao, Quanbo Liu, Jiaqi Xu, Yiming Qin, Rundong He, Feifei Yuan, Tianding Wu, Chunyue Duan, Liyuan Jiang, Hongbin Lu, Jianzhong Hu
Summary: This study explores a specific targeted treatment approach for spinal cord injury by identifying stable subpopulations of exosomes that can improve blood-spinal cord barrier integrity and promote neural function recovery.
Article
Cell & Tissue Engineering
Huangfan Xie, Zhongjie Sun, Xiong Xiao, Defang Liu, Hailong Qi, Guoxiong Tian, Miao Chen, Ligong Chen, Xuncheng Su
Summary: Transient inhibition of the JNK pathway can increase the frequency of HSCs in cord blood and promote their quiescence, leading to the enhancement of HSC number and engraftment potential. These findings have the potential to improve HSC collection and transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Review
Cell & Tissue Engineering
Gabriela Sanchez-Petitto, Katayoun Rezvani, May Daher, Hind Rafei, Partow Kebriaei, Elizabeth J. Shpall, Amanda Olson
Summary: Umbilical cord blood transplantation is an attractive alternative source of hematopoietic stem cells, with unique immune tolerance and engraftment kinetics. It has a lower risk of GvHD and comparable clinical outcomes to bone marrow transplantation. However, limitations in cell numbers and delayed engraftment pose challenges for its wider application. Research is being conducted to address these limitations and explore the potential of cord blood in treating various disorders.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Multidisciplinary Sciences
Linlin Jin, Zimin Sun, Huilan Liu, Xiaoyu Zhu, Yonggang Zhou, Binqing Fu, Xiaohu Zheng, Kaidi Song, Baolin Tang, Yun Wu, Jiang Zhu, Rui Sun, Zhigang Tian, Haiming Wei
Summary: Unrelated cord blood transplantation is effective for hematopoietic disorders but often leads to pre-engraftment syndrome (PES). GM-CSF produced by cord blood-derived inflammatory monocytes drives PES pathology, and tocilizumab has shown clinical efficacy in limiting pathology in PES patients. This study provides insights into the pathogenesis and treatment of PES, improving nonrelapse mortality outcomes.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Hala M. ElBadre, Sahar E. M. El-Deek, Haidi Karam-Allah Ramadan, Mohamed M. Elbadr, Dina Sabry, Noran M. Ahmed, Amr M. Ahmed, Reham El-Mahdy
Summary: In this study, the effects of umbilical cord mesenchymal stem cells (UC-MSCs) derived exosomes on HepG2 cell line and the underlying mechanism of controlling hepatocellular carcinoma (HCC) proliferation were investigated. The results showed that treatment with exosomes significantly reduced the survival of HepG2 cells and altered the expression levels of SIRT-1, SDF-1, CXCR-4, TNF-α, and caspase-3. Furthermore, the anti-proliferative, apoptotic, and anti-angiogenic effects of exosomes on HepG2 cells were time-dependent. These findings suggest that exosomes could be a potential novel therapy for HCC, but further large-scale studies are needed to confirm this conclusion.
Article
Cell & Tissue Engineering
Liang Wang, Huan Li, Jinfu Lin, Ruojie He, Menglong Chen, Yu Zhang, Ziyu Liao, Cheng Zhang
Summary: Overexpression of CCR2 enhanced ADSC muscle homing and therapeutic effects in dystrophic mice following systemic transplantation.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Lindsay Zhou, Courtney McDonald, Tamara Yawno, Graham Jenkin, Suzanne Miller, Atul Malhotra
Summary: Cell therapies using umbilical cord blood and cord tissue have emerged as a promising approach for preventing and treating neonatal morbidities. Published clinical studies mainly focus on safety and feasibility, with limited assessment of efficacy. There are ongoing trials investigating the potential of these therapies in neonates.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Cell & Tissue Engineering
Liem Thanh Nguyen, Nghia Trung Tran, Uyen Thi Trang Than, Minh Quang Nguyen, Anh Minh Tran, Phuong Thi Xuan Do, Thao Thi Chu, Tu Dac Nguyen, Anh Viet Bui, Tien Anh Ngo, Van Thanh Hoang, Nhung Thi My Hoang
Summary: This study evaluated the efficiency of UCB-MSC isolation and expansion using different commercially available media kits and found that StemMACS(TM) MSC Expansion Media is more appropriate. Autologous serum coating and plasma supplement enhanced cell proliferation. UCB-MSCs exhibited functions in promoting angiogenesis and inhibiting cancer cell growth.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Immunology
Yi Chen, Jiamin Huang, Zaiwen Guo, Zhechen Zhu, Yiming Shao, Linbin Li, Yunxi Yang, Yanzhen Yu, Lu Liu, Bingwei Sun
Summary: In this study, the function and heterogeneity of neutrophils in neonatal umbilical cord blood (UCB) were analyzed using single-cell RNA sequencing analysis for the first time. The results showed that neutrophils in UCB were more naive and had more obvious degranulation and activation functions compared to healthy adults. Significant differences were also observed in the amount and function of a specific subset of neutrophils between UCB and healthy adults. Additionally, a new subset of neutrophils with distinct characteristics was identified. This study provides new insights into the mechanism of neutrophil-mediated diseases in neonates and the potential use of neutrophils in UCB.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell & Tissue Engineering
Ying Yang, Ming Zhang, Mengqi Li, Yingchun Li, Wei Yang, Zhuogang Liu, Hongtao Wang
Summary: This study found that adding an unrelated umbilical cord blood unit to haploidentical hematopoietic stem cell transplantation can improve the prognosis and enhance the graft versus leukemia effect without increasing the incidence of graft versus host disease.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Hematology
Asma S. Al-Amoodi, Yanyan Li, Arwa Al-Ghuneim, Hanaa Allehaibi, Ioannis Isaioglou, Luke E. Esau, Dina B. AbuSamra, Jasmeen S. Merzaban
Summary: Compared to short-term (ST) stem cells, long-term (LT) stem cells have difficulties migrating to the bone marrow. This study suggests that enhancing the migration ability of LT-HSCs can overcome this issue.
Article
Biochemistry & Molecular Biology
Mariusz Z. Ratajczak, Mateusz Adamiak, Andrzej Deptala, Joanna Domagala-Kulawik, Janina Ratajczak, Magdalena Kucia
Summary: This article discusses the induction of a sterile inflammation state through the activation of bone marrow stromal and innate immunity cells, which promotes the migration and engraftment of HSPCs. Deciphering these mechanisms will help in understanding the mechanisms of hematopoietic recovery after transplantation, and provide important information for optimizing the clinical process using small molecular modifiers of innate immunity and purinergic signaling.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Biographical-Item
Biophysics
Mariusz Ratajczak, Eliane Gluckman, Robert Peter Gale
BONE MARROW TRANSPLANTATION
(2022)
Article
Environmental Sciences
Katarzyna Waszczuk, Jolanta Kucharska-Mazur, Ernest Tyburski, Katarzyna Rek-Owodzin, Piotr Plichta, Krzysztof Rudkowski, Piotr Podwalski, Tomasz Grazlewski, Monika Mak, Blazej Misiak, Anna Michalczyk, Maciej Tarnowski, Katarzyna Sielatycka, Angelika Szczesniak, Karolina Luczkowska, Barbara Dolegowska, Marta Budkowska, Mariusz Z. Ratajczak, Jerzy Samochowiec
Summary: This study investigates the potential role of stem cells and immune factors in the etiology of schizophrenia. Although preliminary findings suggest the importance of the immune system and regenerative processes, further studies are needed.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biochemistry & Molecular Biology
Kamila Bujko, Mateusz Adamiak, Ahmed Abdelbaset-Ismail, Arjun Thapa, Nicoletta Ilowska, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: This article investigates the role of P2X1 receptor in the trafficking of hematopoietic stem progenitor cells (HSPCs). Through in vitro and in vivo murine models, it is found that deficiency in P2X1 receptor may lead to difficulties in HSPCs homing and could be associated with the intracellular pattern recognition receptor Nlrp3.
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA
(2022)
Meeting Abstract
Hematology
Ahmed Abdelbaset-Ismail, Arjun Thapa, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Meeting Abstract
Hematology
Vira Chumak, Andrzej Ciechanowicz, Mateusz Adamiak, Arjun Thapa, Ahmed Abdelbaset-Ismail, Kamila Bujko, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Meeting Abstract
Hematology
Kamila Bujko, Mateusz Adamiak, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Article
Biochemical Research Methods
Kamila Bujko, Andrzej K. Ciechanowicz, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: Very small embryonic like stem cells (VSELs) are dormant stem cells deposited during embryogenesis in various tissues, including bone marrow. They are released and circulate in peripheral blood at a low level, but increase in number in response to stressors and tissue/organ damage. This study evaluates CD34(+)Lin(-)CD45(-) and CD133(+)Lin(-)CD45(-) VSELs derived from umbilical cord blood, comparing their molecular and proteomic characteristics.
Article
Oncology
Mariusz Z. Ratajczak, Mateusz Adamiak, Ahmed Abdelbaset-Ismail, Kamila Bujko, Arjun Thapa, Vira Chumak, Stephanie Franczak, Katarzyna Brzezniakiewicz-Janus, Janina Ratajczak, Magdalena Kucia
Correction
Oncology
Alison Domingues, Elisa Rossi, Kamila Bujko, Gregoire Detriche, Ulysse Richez, Adeline Blandinieres, Magdalena Kucia, Janina Ratajczak, David M. Smadja, Mariusz Z. Ratajczak
Article
Cell & Tissue Engineering
Arjun Thapa, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: Hematopoietic stem/progenitor cells (HSPCs) express receptors for complement cascade (ComC) cleavage fragments C3a and C5a and respond to inflammation-related cues by sensing PAMPs and DAMPs. The activation of Nox2-ROS-Nlrp3 inflammasome by ComC-derived C3a and C5a plays a role in regulating the responsiveness of HSPCs to stress. Additionally, ComC expressed in HSPCs, known as the complosome, may also contribute to regulating hematopoiesis.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Ahmed Abdelbaset-Ismail, Katarzyna Brzezniakiewicz-Janus, Arjun Thapa, Janina Ratajczak, Magda Kucia, Mariusz Z. Ratajczak
Summary: The study reveals that hematopoietic stem progenitor cells (HSPCs) follow the diurnal circulation rhythm in peripheral blood and melatonin can inhibit the migration of HSPCs by inhibiting Nlrp3 inflammasome activation.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Ahmed Abdelbaset-Ismail, Andrzej K. Ciechanowicz, Kamila Bujko, Janina Ratajczak, Magdalena Kucia, Mariusz Z. Ratajczak
Summary: Proliferation, metabolism, and migration of hematopoietic stem/progenitor cells (HSPCs) are regulated by receptors integrated into membrane lipid rafts (MLRs). The synthesis of MLRs components in HSPCs is regulated by Nox2-ROS-Nlrp3 inflammasome axis, which modulates lipogenesis and the responsiveness of HSPCs to external stimuli.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Himi Tripathi, Alison Domingues, Renee Donahue, Audrey Cras, Coralie L. Guerin, Erhe Gao, Bryana Levitan, Mariusz Z. Ratajczak, David M. Smadja, Ahmed Abdel-Latif, Wadea M. Tarhuni
Summary: Ischemic heart disease, often caused by AMI, is a major cause of morbidity and mortality worldwide. The combination therapy of MSCs and ECFCs can reduce cardiomyocyte apoptosis, scar size, and adverse cardiac remodeling in a pre-clinical model of AMI. This approach shows promise for future human studies during the acute phase of ischemic cardiac injury.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Cell & Tissue Engineering
Vira Chumak, Katarzyna Sielatycka, Andrzej Ciechanowicz, Kamila Bujko, Mariusz Z. Ratajczak, Magdalena Kucia
Summary: Very small embryonic-like stem cells (VSELs) are dormant stem cells in adult tissues that contribute to tissue/organ repair and regeneration. Proteomic analysis revealed that VSELs express proteins involved in development, signal transduction, and cellular functions, supporting their origin from reproductive lineage.
STEM CELL REVIEWS AND REPORTS
(2023)
