Article
Biochemistry & Molecular Biology
Ilona Faustova, Kaidi Moll, Ervin Valk, Mart Loog, Mihkel Ord
Summary: Cyclins not only activate CDK complexes, but also serve as docking scaffolds for CDK substrates and inhibitors. G1-cyclins in yeast play a specific role in promoting bud growth and polarization, essential for cell survival. The discovery of a specific docking motif in G1-cyclins expands our understanding of cyclin specificity mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Xingfeng Xu, Mao Li, Yan Su, Qi Wang, Peifang Qin, Haitao Huang, Yuting Zhang, Yali Zhou, Jianguo Yan
Summary: This article aimed to explore the potential mechanism of C9orf72 involved in the pathogenesis of Parkinson's disease (PD). The study found that the expression level of C9orf72 protein was significantly reduced in PD models, while the mRNA expression level remains unchanged. The degradation of C9orf72 protein was mainly through the ubiquitin-proteasome pathway, and its phosphorylation at the S9 site promoted its degradation. Knockdown of C9orf72 aggravated autophagy dysfunction and increased neuronal loss and motor dysfunction in PD mice, while overexpression of C9orf72 alleviated autophagy dysfunction in PD neurons. Interfering with Cdk5 phosphorylation at the S9 site of C9orf72 could alleviate autophagy dysfunction, neuronal death, and motor dysfunction caused by C9orf72 protein degradation.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Xiaolin Xu, Chenglong Wang, Peipei Zhang, Xuzhu Gao, Wencai Guan, Fanchen Wang, Xin Li, Jia Yuan, Hongjing Dou, Guoxiong Xu
Summary: This study reveals that chemoresistant cancer cells show decreased ROS levels and increased oxidative phosphorylation. Chemoresistant cells generate more energy through oxidative phosphorylation and enhance ROS scavenging systems to resist the effects of chemotherapy. Furthermore, increased ROS production efficiently inhibits chemoresistance and promotes cell death in chemoresistant cancer cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Dionysia Petratou, Martha Gjikolaj, Eva Kaulich, William Schafer, Nektarios Tavernarakis
Summary: The nervous system plays a crucial role in systemic stress response and maintaining neuronal sodium homeostasis. Imbalance in neuronal sodium homeostasis is linked to nervous system pathologies, but the effects of stress on this balance are not well understood. This study reveals that the DEG/ENaC family member DEL-4 functions as a proton-inactivated sodium channel in neuronal membranes and synapses, modulating locomotion in Caenorhabditis elegans. Heat stress and starvation influence DEL-4 expression, which in turn affects the expression and activity of stress-response transcription factors and leads to appropriate motor adaptations. Additionally, DEL-4 deficiency affects dopaminergic neurons and neurotransmission, while promoting neuronal survival in models of neurodegenerative diseases. These findings provide insights into the molecular mechanisms underlying the role of sodium channels in neuronal function and adaptation during stress.
Article
Physiology
Jiqiang Song, Yanping Yu, Zhichao Yan, Shan Xiao, Xianxin Zhao, Fang Wang, Qi Fang, Gongyin Ye
Summary: This study investigated the characteristics and functions of CLIC in Hi-5 cells, and found that the TnCLIC protein is essential for the survival of Hi-5 cells and has a lethal effect when knocked down in different insect cell lines. It was also discovered that the knockdown of TnCLIC leads to an increase in intracellular calcium ion levels. These results provide insights into the crucial role of insect CLIC in cell survival and lay the foundation for understanding the cell death mechanism.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Neurosciences
Qingqing Bai, Shuoyang Wang, Dongmei Rao, Zhiming Zhou, Jianfei Wang, Qi Wang, Yu Qin, Zhaohu Chu, Shoucai Zhao, Dijing Yu, Yang Xu
Summary: In this study, the researchers investigated the impact of RIPK3 activation on neuronal necroptosis and the involvement of DAXX in the nuclear necroptosis pathway after ICH. The results showed that RIPK3 promoted neuronal necroptosis in ICH mice, partially through the DAXX signaling pathway. RIPK3 and AIF interacted with nuclear DAXX to aggravate ICH injury.
CNS NEUROSCIENCE & THERAPEUTICS
(2023)
Article
Medicine, Research & Experimental
Ruonan Ma, Ling Fang, Lei Chen, Xiaonan Wang, Jing Jiang, Lizeng Gao
Summary: This study explores the role of iron in macrophages in suppressing intracellular bacteria. It reveals that the induction of ferroptotic stress and the inhibition of iron transport can lead to the death of intracellular bacteria. The findings suggest that targeting ferroptosis may provide a therapeutic approach for infectious diseases caused by intracellular pathogens.
Article
Biochemistry & Molecular Biology
Tao Zhang, Yongfang Xia, Li Hu, Dongmei Chen, Chen-Ling Gan, Long Wang, Yingxue Mei, Guihua Lan, Xindong Shui, Yuan Tian, Ruomeng Li, Mi Zhang, Tae Ho Lee
Summary: The aggregation of A13 peptides into oligomers and fibrils is a key feature of Alzheimer's disease. This study reveals that A13 oligomers can upregulate DAPK1 levels and cause neuronal apoptosis and tau phosphorylation. The inhibition of DAPK1 can protect neurons against A13-induced damage.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ilona Faustova, Luka Bulatovic, Frida Matiyevskaya, Ervin Valk, Mihkel Ord, Mart Loog
Summary: Cyclin-dependent kinases (CDKs) are regulated by different cyclins throughout the cell cycle, with the NLxxxL docking motif playing a crucial role in the phosphorylation-dependent degradation of the CDK inhibitor Far1 at the G1/S stage in yeast. This motif is specifically recognized by the S-phase CDK (S-CDK) Clb5/6-Cdc28, indicating its importance in controlling cell cycle events. Additionally, the NLxxxL motif governs S-cyclin-specificity in multiple yeast CDK targets, suggesting its significance in fine-tuning cell cycle processes.
Article
Biology
Dianrong Li, Jie Chen, Jia Guo, Lin Li, Gaihong Cai, She Chen, Jia Huang, Hui Yang, Yinhua Zhuang, Fengchao Wang, Xiaodong Wang
Summary: The study reveals that RIPK3 signals to either necroptosis or apoptosis depending on its serine 165/threonine 166 phosphorylation status, with low levels of the chaperone Hsp90/CDC37 leading to RIPK3 also signaling to apoptosis. Dysfunction in RIPK3 phosphorylation can impact cellular fate, as demonstrated by knock-in mutations in mice.
Article
Biochemistry & Molecular Biology
Hongmei Wang, Siyuan Bu, Jiajian Tang, Yi Li, Chunhua Liu, Junhong Dong
Summary: The protein tyrosine phosphatase PTPN5 is expressed in a developmental manner in the murine pituitary, and its expression is significantly reduced in the pituitary after ovary removal. PTPN5 regulates FSH secretion through two parallel signaling pathways and is activated by Ca2+-dependent phosphatase calcineurin. Blocking or knocking out PTPN5 reduces FSH release in the whole pituitary by regulating intracellular calcium homeostasis.
Review
Cell Biology
Diksha Kumari, Krishanu Ray
Summary: Phosphorylation of Kinesin subunits and adaptors plays a crucial role in regulating the activity and interactions of Kinesin motors, influencing intracellular transport dynamics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mihee Oh, Sun Young Kim, Jeong-Su Byun, Seonha Lee, Won-Kon Kim, Kyoung-Jin Oh, Eun-Woo Lee, Kwang-Hee Bae, Sang Chul Lee, Baek-Soo Han
Summary: JAK2 may negatively regulate neuronal differentiation by suppressing the GSK-3 beta/Fyn/CDK5 signaling pathway responsible for morphological maturation in the nervous system.
Article
Biochemistry & Molecular Biology
Shuzhen Liu, Preston Perez, Xue Sun, Ken Chen, Rojin Fatirkhorani, Jamila Mammadova, Zhigao Wang
Summary: MLKL forms amyloid-like polymers to promote necroptosis, which induces lysosomal membrane permeabilization (LMP) and subsequent cell death. Cathepsin B plays a significant role in this process and its inhibition protects cells from necroptosis.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Medicine, Research & Experimental
Ke-Jie He, Yang-fan Zhang, Lai-ying Liang, Xiao-Shen Cheng, Guoyu Gong, Xiao-Mei Ouyang, Ying Lin, Bayasi Guleng
Summary: This study evaluated the function and prognosis of TICRR in liver cancer and found that it could serve as an independent prognostic marker. TICRR was also found to be related to immune infiltration and had a positive correlation with PD1/PD-L1 and CTLA-4. Additionally, experiments demonstrated that TICRR facilitated the proliferation and metastasis of liver cancer cells in vitro. These findings suggest that TICRR might be a potential biomarker and therapeutic target in liver cancer.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)