Article
Hematology
Anna K. Brandtner, Georg F. Lehner, Andreas Pircher, Clemens Feistritzer, Michael Joannidis
Summary: In this study, it was found that inflammation can induce endothelial cells to express tissue factor and trigger a procoagulant state, with the highest TF-PCA observed in microvascular endothelial cells. The correlation between IL-6 and TF levels was positive in a cohort of septic patients. The ratio of TF to its inhibitor TFPI on the endothelial membrane differs between endothelial cell subtypes, possibly explaining the susceptibility of the microvascular system to inflammation-induced thrombosis.
THROMBOSIS RESEARCH
(2021)
Article
Medicine, Research & Experimental
Yu-Hang Liu, Lorenz M. Brunner, Johannes Rebling, Maya Ben-Yehuda Greenwald, Sabine Werner, Michael Detmar, Daniel Razansky
Summary: This study used non-invasive imaging technology to observe and monitor the process of skin repair in mice, and found that overexpression of vascular endothelial growth factor A (VEGF-A) can promote vascularization in the skin, which was further validated by detailed histological staining results.
Article
Multidisciplinary Sciences
Sara G. Romeo, Ilaria Secco, Edoardo Schneider, Christina M. Reumiller, Celio X. C. Santos, Anna Zoccarato, Vishal Musale, Aman Pooni, Xiaoke Yin, Konstantinos Theofilatos, Silvia Cellone Trevelin, Lingfang Zeng, Giovanni E. Mann, Varun Pathak, Kevin Harkin, Alan W. Stitt, Reinhold J. Medina, Andriana Margariti, Manuel Mayr, Ajay M. Shah, Mauro Giacca, Anna Zampetaki
Summary: The microvasculature is critical for the delivery of oxygen and metabolites throughout tissues. In this study, human blood vessel organoids (BVOs) were used to show that CTGF is a critical paracrine regulator of microvascular integrity that can restore pericyte coverage and vessel structure.
NATURE COMMUNICATIONS
(2023)
Article
Hematology
Kathryn M. Musgrave, Jonathan Scott, Wezi Sendama, Aaron I. Gardner, Fiona Dewar, Cameron J. Lake, Henri M. H. Spronk, Rene van Oerle, Mayken Visser, Hugo ten Cate, Patrick Kesteven, Andrew Fuller, David McDonald, Carly Knill, Gillian Hulme, Andrew Filby, Stephen E. Wright, Alistair I. Roy, Marie-Helene Ruchaud-Sparagano, A. John Simpson, Anthony J. Rostron
Summary: The expression of tissue factor varies in different subsets of monocytes. Stimulation with lipopolysaccharide reduces the proportion of intermediate monocytes and increases tissue factor expression on classical and non-classical monocytes. In critically ill patients, tissue factor expression is significantly higher in intermediate and non-classical monocytes among patients with sepsis. After recovery, tissue factor expression increases significantly in classical monocytes.
THROMBOSIS RESEARCH
(2023)
Review
Oncology
Erika Testa, Claudia Palazzo, Roberta Mastrantonio, Maria Teresa Viscomi
Summary: This review discusses the dynamic interaction between tumor cells and brain microvascular endothelial cells (BMECs), providing evidence of how tumor cells utilize BMECs for the formation of new blood vessels. The interplay between tumor cells and BMECs involves multiple communication routes, including soluble factors, direct cell-cell contact, and extracellular vesicles. Furthermore, the review describes how this interaction modifies the structure, metabolism, and physiology of BMECs to promote tumor growth and invasiveness. Recent advances in high-throughput 3D models are briefly discussed for a better understanding of the complexity of BMECs-tumor cell interaction.
Article
Neurosciences
Wen Zeng, Qiaoling Lei, Jiao Ma, Shuqiang Gao, Rong Ju
Summary: This study investigated the biological effects of EPC-derived MVs on BMECs, showing that EPC-MVs can enhance BMEC viability, proliferation, migration, and tube formation. These results suggest the proangiogenic effect of EPC-derived MVs on BMECs, which holds potential applications in cell-free therapy for brain injury.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Cardiac & Cardiovascular Systems
Gemma Arderiu, Esther Pena, Lina Badimon
Summary: In this study, it was shown that ischaemia-activated endothelial cells release microvesicles rich in tissue factor (TF) which promote monocyte differentiation into endothelial cell-like cells and new blood vessel formation in the ischaemic zone. This mechanism may contribute to tissue repair in ischaemic conditions.
CARDIOVASCULAR RESEARCH
(2022)
Article
Endocrinology & Metabolism
Xu Jing, Jieyu Wu, Caijuan Dong, Juan Gao, Takahiro Seki, Changil Kim, Egon Urgard, Kayoko Hosaka, Yunlong Yang, Siwen Long, Ping Huang, Junnian Zheng, Laszlo Szekely, Yuanting Zhang, Wei Tao, Jonathan Coquet, Minghua Ge, Yuguo Chen, Mikael Adner, Yihai Cao
Summary: COVID-19 infection enhances adipose browning and non-shivering thermogenesis, leading to adipose atrophy and weight loss. Inhibition of vascular endothelial growth factor (VEGF) signaling may represent an effective approach for preventing and treating COVID-19-associated weight loss.
Review
Endocrinology & Metabolism
Rick H. G. J. van Lanen, Stan Melchers, Govert Hoogland, Olaf E. M. G. Schijns, Marc A. M. J. van Zandvoort, Roel H. L. Haeren, Kim Rijkers
Summary: The blood-brain barrier is dysfunctional in temporal lobe epilepsy, likely due to microvascular changes. Vascular endothelial growth factor (VEGF) plays a pivotal role in this process.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Cell Biology
Vincent Roy, Alyssa Brodeur, Lydia Touzel Deschenes, Nicolas Dupre, Francois Gros-Louis
Summary: The role of RNF213 in MMD pathology has been studied using a CRISPR-Cas9 system to generate a knockout model in human cerebral microvascular endothelial cells. This in vitro model successfully replicated MMD-associated features such as increased angiogenesis and secretion of pro-angiogenic factors, highlighting their potential as biomarkers.
Review
Biochemistry & Molecular Biology
Sharmila Rajendran, Shanmuganathan Seetharaman, Arun Dharmarajan, Kaviarasan Kuppan
Summary: Pericytes are microvascular mural cells that regulate vascular development, stabilization, maturation, and remodeling. In diabetic retinopathy and diabetic nephropathy, apoptotic loss and migration of pericytes lead to microvascular instability, exacerbating the disease progression. Specific biomarkers are needed for early stabilization of pericytes in these diabetes-associated complications.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Review
Physiology
Jacqueline Herold, Joanna Kalucka
Summary: Obesity leads to expansion of adipose tissue, affecting its angiogenic capacity and increasing the risk of obesity-related diseases such as cardiovascular diseases.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Dermatology
Jiao Li, Hui Hou, Ling Zhou, Juanjuan Wang, Jiannan Liang, Junqin Li, Ruixia Hou, Xuping Niu, Guohua Yin, Xinhua Li, Kaiming Zhang
Summary: The study found that psoriatic HDMECs exhibit increased tube formation and cell migration, as well as reduced proliferation and metabolic activity compared to normal HDMECs.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Immunology
Gemma Arderiu, Esther Pena, Anna Civit-Urgell, Lina Badimon
Summary: Hypoxic endothelial cells release extracellular microvesicles that induce monocyte homing and angiogenesis. The microvesicles contain noncoding RNAs, including miR-126, which can modulate cellular signaling pathways. Activated endothelial cells secrete microvesicles carrying miR-126, which regulate monocyte reprogramming of angiogenic genes.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Arinola O. Lampejo, Nien-Wen Hu, Daniela Lucas, Banks M. Lomel, Christian M. Nguyen, Carmen C. Dominguez, Bing Ren, Yong Huang, Walter L. Murfee
Summary: The development of biomimetic models bridges the gap between in vitro and in vivo assays, providing tools for studying microvascular dynamics. A key challenge is to recreate the functional complexity of a real network environment. Engineering design requirements and top-down tissue culture methods help achieve the matching of physiological function.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cardiac & Cardiovascular Systems
Sean M. Davidson, Chantal M. Boulanger, Elena Aikawa, Lina Badimon, Lucio Barile, Christoph J. Binder, Alain Brisson, Edit Buzas, Costanza Emanueli, Felix Jansen, Miroslava Katsur, Romaric Lacroix, Sai Kiang Lim, Nigel Mackman, Manuel Mayr, Philippe Menasche, Rienk Nieuwland, Susmita Sahoo, Kaloyan Takov, Thomas Thum, Pieter Vader, Marca H. M. Wauben, Kenneth Witwer, Joost P. G. Sluijter
Summary: This article provides an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. It discusses methods for determining the protein, RNA, and lipid content of EVs. The guidance provided in this document is important for investigating the role of EVs in cardiovascular diseases and highlights key methodological issues to consider.
CARDIOVASCULAR RESEARCH
(2023)
Review
Cardiac & Cardiovascular Systems
Frank Lezoualc'h, Lina Badimon, Hana Baker, Monique Bernard, Gabor Czibik, Rudolf A. de Boer, Thomas D'Humieres, Micheline Kergoat, Mark Kowala, Jennifer Rieusset, Gemma Vilahur, Maximin Detrait, Chris Watson, Genevieve A. Derumeaux
Summary: Diabetic cardiomyopathy is a distinct, multifactorial disease that leads to ventricular hypertrophy and abnormal myocardial contractility. Animal models provide opportunities to study its mechanisms, but there are limitations when extrapolating findings to humans.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cardiac & Cardiovascular Systems
Raffaele Bugiardini, Stefano Nava, Gaetano Caramori, Jinsung Yoon, Lina Badimon, Maria Bergami, Edina Cenko, Antonio David, Ilir Demiri, Maria Dorobantu, Oana Fronea, Radmilo Jankovic, Sasko Kedev, Nebojsa Ladjevic, Ratko Lasica, Goran Loncar, Giuseppe Mancuso, Guiomar Mendieta, Davor Milicic, Petra Mjehovic, Marijan Pasalic, Milovan Petrovic, Lidija Poposka, Marialuisa Scarpone, Milena Stefanovic, Mihaela van der Schaar, Zorana Vasiljevic, Marija Vavlukis, Maria Laura Vega Pittao, Vladan Vukomanovic, Marija Zdravkovic, Olivia Manfrini
Summary: This retrospective cross-sectional study in 17 hospitals across 5 European countries found that women were at higher risk of in-hospital mortality and acute heart failure compared to men, but sex was not associated with mortality risk in patients receiving intensive care treatment for COVID-19.
CARDIOVASCULAR RESEARCH
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Emiel P. C. van der Vorst, Lina Badimon
CARDIOVASCULAR RESEARCH
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Lina Badimon, Guiomar Mendieta, Gemma Vilahur
EUROPEAN HEART JOURNAL
(2023)
Article
Medicine, General & Internal
Maria Carmen Garcia-Gomez, Teresa Padro, Natalia Munoz-Garcia, Maria Bianchi, Lorenzo Alvarez, Lina Badimon, Emili Corbella, Xavier Pinto
Summary: This study investigated the HDL antioxidant function in patients with rheumatoid arthritis (RA). The results showed that RA patients had lower HDL antioxidant capacity and a lower resistance of LDL particles to oxidation, mainly related to the degree of inflammation.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Alberto Cordero, Natalia Munoz-Garcia, Teresa Padro, Gemma Vilahur, Vicente Bertomeu-Gonzalez, David Escribano, Emilio Flores, Pilar Zuazola, Lina Badimon
Summary: This study investigated the role of lipoprotein functionality as well as particle number and size in patients with acute coronary syndrome (ACS) and on-target LDL-C levels. It was found that ACS patients had higher susceptibility to LDL oxidation and lower HDL-antioxidant capacity. They also had lower HDL-C and Apolipoprotein A-I levels compared to non-ACS patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Alba Vilella-Figuerola, Alberto Cordero, Sonia Mirabet, Natalia Munoz-Garcia, Rosa Suades, Teresa Padro, Lina Badimon
Summary: This study investigated platelet-derived extracellular vesicles (pEVs) in patients with chronic heart failure (CHF) and first-onset acute coronary syndrome (ACS). It was found that CHF patients had reduced pEVs carrying specific antigens, while ACS patients had increased pEVs with phosphatidylserine (PS) exposure. The background etiology of CHF or ACS type did not affect pEV levels.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Alex Gallinat, Gemma Vilahur, Teresa Padro, Lina Badimon
Summary: This study analyzed the effects of beer consumption on the gene expression response of the heart under oxidative stress, and found that beer intake can regulate gene expression in the heart and have a protective effect. It was also found that different doses of beer intake have different effects.
Article
Cardiac & Cardiovascular Systems
Jeanine E. Roeters van Lennep, Lale S. Tokgozoglu, Lina Badimon, Sandra M. Dumanski, Martha Gulati, Connie N. Hess, Kirsten B. Holven, Maryam Kavousi, Meral Kayikcioglu, Esther Lutgens, Erin D. Michos, Eva Prescott, Jane K. Stock, Anne Tybjaerg-Hansen, Marieke J. H. Wermer, Marianne Benn
Summary: Cardiovascular disease is the leading cause of death globally, with atherosclerotic cardiovascular disease (ASCVD) being the main contributor. ASCVD mortality is currently increasing, especially in middle-aged women. Sex-specific factors, such as hypertensive disorders of pregnancy, premature menopause, and polycystic ovary syndrome, are associated with higher cardiovascular risk.
EUROPEAN HEART JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Rosa Suades, Alba Vilella-Figuerola, Teresa Padro, Sonia Mirabet, Lina Badimon
Summary: In this study, circulating extracellular microvesicles (cEVs) from chronic heart failure patients were phenotypically and quantitatively characterized. It was found that PS--cEVs were predominantly present in both chronic heart failure patients and non-HF subjects, but the proportions of PS-cEVs varied depending on the parental markers. PS+-cEVs were mainly derived from platelets and increased in the non-HF group, while PS--cEVs were mainly derived from endothelium and connexin 43-rich sources and significantly increased in chronic heart failure patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Maria Bergami, Olivia Manfrini, Stefano Nava, Gaetano Caramori, Jinsung Yoon, Lina Badimon, Edina Cenko, Antonio David, Ilir Demiri, Maria Dorobantu, Natalia Fabin, Oana Gheorghe-Fronea, Radmilo Jankovic, Sasko Kedev, Nebojsa Ladjevic, Ratko Lasica, Goran Loncar, Giuseppe Mancuso, Guiomar Mendieta, Davor Milicic, Petra Mjehovic, Marijan Pasalic, Milovan Petrovic, Lidija Poposka, Marialuisa Scarpone, Milena Stefanovic, Mihaela van der Schaar, Zorana Vasiljevic, Marija Vavlukis, Maria Laura Vega Pittao, Vladan Vukomanovic, Marija Zdravkovic, Raffaele Bugiardini
Summary: This study found that the use of azithromycin in COVID-19 patients with prior history of cardiovascular disease is significantly associated with an increased risk of acute heart failure and all-cause 30-day mortality. However, in patients without prior cardiovascular disease, azithromycin treatment is associated with significantly reduced risk of death and no significant increase in acute heart failure.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2023)
Article
Medical Laboratory Technology
Lola Fernandez Encinas, Nuria Lluch, Alan H. B. Wu, Juan Carlos Kaski, Lina Badimon, Judit Cubedo
Summary: This study aimed to validate the analytical performance of two novel ELISAs for two different glycosylated ApoJ variants (ApoJ-GlycA2 and ApoJ-GlycA6). The results showed that these methods fulfilled the acceptance criteria established by the European Medicines Agency for precision, accuracy, recovery, cross-reactivity, and stability. These newly developed ELISAs accurately measured serum levels of ApoJ-GlycA2 and ApoJ-GlycA6.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Gemma Arderiu, Anna Civit-Urgell, Lina Badimon
Summary: The incidence and prevalence of critical limb ischemia have increased, but there are no successful treatments available. Adipose-derived stem cells (ASCs) are a promising source of stem cells for cell-based therapy due to their multilineage capacity and low immunogenicity. The proangiogenic benefits of ASCs are attributed to their secretion of proangiogenic molecules, microvesicles/exosomes, and their differentiation capability toward endothelial cells (ECs). However, the therapeutic efficacy of ASCs in peripheral artery diseases patients is still relatively low.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Jeanine E. Roeters van Lennep, Lale S. Tokgozoglu, Lina Badimon, Sandra M. Dumanski, Martha Gulati, Connie N. Hess, Kirsten B. Holven, Maryam Kavousi, Meral Kayikcioglu, Esther Lutgens, Erin D. Michos, Eva Prescott, Jane K. Stock, Anne Tybjaerg-Hansen, Marieke J. H. Wermer, Marianne Benn
Summary: Cardiovascular disease is the leading cause of death globally, with women experiencing the fastest relative increase in middle-aged mortality. In addition to missed or delayed diagnosis and undertreatment, sex-specific factors contribute to increased cardiovascular risk in women. The European Atherosclerosis Society's position statement focuses on the impact of gender-specific factors and the effects of these factors on women's lifelong lipid profiles, including lipoprotein(a). Sociocultural components further compound these effects. Early identification and treatment of modifiable cardiovascular risk factors in women, especially those related to sex-specific conditions, are necessary to reduce the high burden of cardiovascular disease in women.
EUROPEAN HEART JOURNAL
(2023)