Article
Biotechnology & Applied Microbiology
Emily E. Jackson, Nathalie Gnanou Besse, Rabeb Bennour Hennekinne, Timothy Ells, Michel Laurentie, Thomas Meheut, Stephen J. Forsythe
Summary: This study reports the sequence types of Cronobacter sakazakii strains and investigates the correlation between sequence type and growth rate. The results suggest that there is no strong correlation between the two factors.
Article
Food Science & Technology
Jae-Ik Lee, Sang-Soon Kim, Dong-Hyun Kang
Summary: In this study, the effect of desiccation stress on the transcriptomic and phenotypic changes of Cronobacter sakazakii ATCC 29544 was investigated. The addition of glucose or lactose to the growth medium induced differentially expressed genes (DEGs) and altered the membrane lipids, making C. sakazakii more susceptible to lipid oxidation. The transcriptome and phenotype of C. sakazakii were found to be influenced by the nutritional materials used to cultivate the pathogen.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Zhanwen Zheng, Yawen Xie, Sheng Ma, Junhong Tu, Jiahui Li, Sen Liang, Yunfeng Xu, Chao Shi
Summary: The study demonstrated that 405-nm LED light effectively inactivated C. sakazakii in PIF, reducing its tolerance to various environmental stressors. LED illumination also downregulated the transcription levels of tolerance-associated genes and caused damage to bacterial cell membranes. This suggests the potential of 405-nm LED technology in preventing and controlling pathogens in food processing and storage environments.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Agriculture, Dairy & Animal Science
Ju Liu, Guoyang Xie, Qin Xiong, Dan Mu, Hengyi Xu
Summary: A simple and sensitive fluorescent turn-off aptasensor was developed for detecting viable Cronobacter sakazakii in powdered infant formula. The sensor showed good performance and specificity in detecting the pathogen within 3 hours in both pure culture and formula samples.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Immunology
Margaret G. Taylor, Megan H. Amerson-Brown, Kristina Hulten, Lindsay H. Cameron, Galit Holzmann-Pazgal, Morven S. Edwards, Catherine E. Foster
Summary: This study reports two cases of infants hospitalized with Cronobacter sakazakii meningitis, both of whom had exposure to powdered infant formula at home. Early advanced brain imaging is recommended, and reporting to public health officials may help identify outbreaks.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2021)
Article
Food Science & Technology
Yuan-song Zhang, Xuechao Xu, Juanli Yang, Mengyuan Tan, Wenyuan Zhou, Lu Gao, Zhen-quan Yang
Summary: In this study, a chromogenic system based on phage-directed immobilized nanozyme was developed for colorimetric measurement of Cronobacter sakazakii in powdered infant formula (PIF). The method showed high specificity and low detection limit, successfully detecting C. sakazakii and achieving good recovery rates.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Haiyan Zeng, Chengsi Li, Dandan Luo, Jumei Zhang, Yu Ding, Moutong Chen, Xiaojuan Yang, Tao Lei, Shi Wu, Qinghua Ye, Rui Pang, Qihui Gu, Qingping Wu
Summary: A novel lytic phage B1 was isolated from river water in Guangzhou, China, which can simultaneously lyse C. malonaticus and C. turicensis, showing potential for controlling foodborne pathogens. The phage has high stability, strong lytic ability, and can significantly inhibit the growth of Cronobacter spp., especially C. turicensis, making it a promising candidate for food safety.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Doyeon Kim, Minsik Kim
Summary: In this study, we developed a bioluminescent reporter bacteriophage for rapid and specific detection of Cronobacter sakazakii (Cs) in food and food processing environments.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Hyung Suk Kim, Md. Ashrafudoulla, Bo-Ram Kim, Md. Furkanur Rahaman Mizan, Soo-Jin Jung, Mohammad Sadekuzzaman, Si Hong Park, Sang-Do Ha
Summary: The study showed that bacteriophages PBES04 and PBES19 can effectively reduce the biofilm formation of Cronobacter sakazakii on stainless steel and silicone rubber surfaces. C. sakazakii was not detected in infant formula milk treated with these bacteriophages, indicating their potential effectiveness in increasing food safety in commercial facilities.
Article
Food Science & Technology
Jae-Ik Lee, Sang-Soon Kim, Dong-Hyun Kang
Summary: The aim of this study was to develop a new primer-probe set for detecting Cronobacter sakazakii based on comparative genomics. The sensitivity and selectivity of the designed primer were confirmed, and it was found to have minimal interference from other substances. Additionally, the detection efficiency of the designed primer in various food samples was comparable to that of conventionally used selective media.
Review
Food Science & Technology
Rajni Chauhan, Ben D. Tall, Gopal Gopinath, Wamik Azmi, Gunjan Goel
Summary: This article reviews the factors and strategies responsible for the survival and persistence of Cronobacter sakazakii in powdered infant formula (PIF), focusing on thermal resistance, antibiotic or antimicrobial resistance, virulence potential, and stress-associated gene expression. This pathogen poses a high risk to neonates and its ability to survive in low-moisture foods is influenced by intrinsic and extrinsic factors.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Food Science & Technology
Geng Zou, Libere Ndayishimiye, Lingxiang Xin, Manshan Cai, Longjian Zhang, Jie Li, Zhiyong Song, Renwei Wu, Yang Zhou, Yuanguo Shi, Yingwang Ye, Rui Zhou, Jinquan Li
Summary: In this study, a phage termed LPCS28 with a broad lysis spectrum against C. sakazakii was isolated from environmental water samples. LPCS28 showed high thermal stability, with no significant decrease in titer after heating at 60 degrees C for one hour. Upon thermal processing at 63 degrees C for 30 min, LPCS28 effectively inhibited C. sakazakii in reconstituted powdered infant formula and liquid milk, reducing its concentration to below the limit of detection within 9 h at 37 degrees C. Phylogenetic analysis classified LPCS28 as a new genus, Nanhuvirus, within the family Straboviridae. These results highlight the potential of LPCS28 as a biological control agent against pathogenic C. sakazakii in the dairy industry.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Lihan Wang, Xinyi Pang, Jiayuan Zhao, Haonan Jin, Xinyan Yang, Shiqian Fu, Shasha Cheng, Hongxuan Li, Chao Miao, Chaoxin Man, Yujun Jiang
Summary: JK004 is a novel virulent phage isolated from the native environment of C. sakazakii, known for its tolerance to heat, pH, and osmotic-stress. It shows excellent antibacterial activity against C. sakazakii and biofilms, without containing any known virulence, transduction, or antibiotic resistance genes, indicating its safety for practical applications in food production. The study suggests the potential of JK004 as an effective strategy for controlling C. sakazakii.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Yanpeng Yang, Sheng Ma, Kailun Guo, Du Guo, Jiahui Li, Muxue Wang, Yutang Wang, Chunling Zhang, Xiaodong Xia, Chao Shi
Summary: This study found that combined LED illumination and citral treatment can effectively reduce the abundance of C. sakazakii in reconstituted powdered infant formula. The combination treatment also destroys cellular morphology and membrane integrity, and induces lipid peroxidation. Neither LED illumination nor citral treatment results in DNA breakdown in C. sakazakii.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Microbiology
Pauline Ogrodzki, Stephen J. Forsythe
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Microbiology
Maha A. Aldubyan, Ibtesam S. Almami, Fatiha M. Benslimane, Abdlrhman M. Alsonosi, Stephen J. Forsythe
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Microbiology
Peng Fei, Yichao Jiang, Yan Jiang, Xiujuan Yuan, Tongxiang Yang, Junliang Chen, Ziyuan Wang, Huaibin Kang, Stephen J. Forsythe
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Immunology
Ondrej Holy, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Igor Hochel, Julio Parra-Flores, Jana Petrzelova, Kamila Facevicova, Stephen Forsythe, Abdlrhman Alsonosi
MICROBIAL PATHOGENESIS
(2019)
Article
Dentistry, Oral Surgery & Medicine
M. A. Payne, A. Hashim, A. Alsam, S. Joseph, J. Aduse-Opoku, W. Wade, M. A. Curtis
JOURNAL OF DENTAL RESEARCH
(2019)
Article
Microbiology
Susan M. Joseph, Thomas Battaglia, Julia M. Maritz, Jane M. Carlton, Martin J. Blaser
Article
Immunology
L. Monin, D. S. Ushakov, H. Arnesen, N. Bah, A. Jandke, M. Munoz-Ruiz, J. Carvalho, S. Joseph, B. C. Almeida, M. J. Green, E. Nye, S. Hatano, Y. Yoshikai, M. Curtis, H. Carlsen, U. Steinhoff, P. Boysen, A. Hayday
MUCOSAL IMMUNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
P. V. Costa, R. M. de Siqueira, A. C. Rosa Guimaraes, L. Vasconcellos, V. Midlej, G. M. Silva da Conceicao, S. J. Forsythe, M. L. Lima Brandao
Summary: This study evaluated the cytotoxic activity of Cronobacter strains isolated from foods and clinical samples in Brazil, and identified some strains capable of producing cytotoxins.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Food Science & Technology
Na Ling, Yujun Jiang, Haiyan Zeng, Yu Ding, Stephen Forsythe
Summary: This review discusses the progress made in China regarding the research on Cronobacter, including large-scale surveys and the adoption of DNA sequence-based methods for identification and genotyping of the organism. These methods have significantly advanced understanding of the taxonomy, ecology, and virulence of Cronobacter, leading to improved source tracking in infant formula production facilities and epidemiological investigations.
JOURNAL OF FOOD SCIENCE
(2021)
Review
Dentistry, Oral Surgery & Medicine
Susan Joseph, Michael A. Curtis
Summary: Recent advances in research on microbial populations in the human mouth provide new insights into the mechanisms underlying periodontal health and disease. The resilience of healthy and diseased microbial communities may be influenced by the genetics of the host and the makeup of the acquired microbiome.
PERIODONTOLOGY 2000
(2021)
Article
Microbiology
Susan Joseph, Joseph Aduse-Opoku, Ahmed Hashim, Eveliina Hanski, Ricarda Streich, Sarah C. L. Knowles, Amy B. Pedersen, William G. Wade, Michael A. Curtis
Summary: A curated murine oral microbiome database has been constructed, containing a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa. The database comprises 103 mouse oral taxa (MOT) spanning four phyla, including 12 novel undescribed species-level taxa. Key observations include the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome, as well as the identification of three major murine-specific oral bacterial lineages.
Article
Immunology
A. Hashim, A. Alsam, M. A. Payne, J. Aduse-Opoku, M. A. Curtis, S. Joseph
Summary: Neutrophils play a crucial role in regulating the oral microbiota, with their deficiency predisposing individuals to periodontal disease. Without neutrophils, there is a significant shift in the oral microbiome composition, leading to increased periodontal bone pathology. However, transferring the oral microbiome of neutrophil-deficient mice back to wild-type mice can restore the microbiome composition and prevent pathology.
INFECTION AND IMMUNITY
(2021)
Review
Agriculture, Dairy & Animal Science
Lihan Wang, Stephen J. Forsythe, Xinyan Yang, Shiqian Fu, Chaoxin Man, Yujun Jiang
Summary: Cronobacter genus includes food-borne pathogens that can cause severe infections in infants, with a high mortality rate. Advanced detection technology and analysis of high-frequency species from different sources can aid in targeted control of Cronobacter. The bacteria's resistance to environmental stresses and the development of new control techniques highlight the importance of strict monitoring and prevention throughout food processing and storage.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
S. Joseph, M. Carda-Dieguez, J. Aduse-Opoku, A. Alsam, A. Mira, M. A. Curtis
Summary: Periodontal disease leads to changes in cellular profiles and biological activities of both the subgingival microbiome and host tissues. Analysis of a murine periodontal disease model using metatranscriptomics revealed differential expression of 3,468 host transcripts, with 76% being overexpressed in periodontitis. Disease was associated with alterations in immune and inflammatory genes and pathways, as well as cellular/metabolic processes and biological regulation. These metatranscriptome data provide insights into gene expression patterns in periodontal disease and can be used for future functional studies.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Microbiology
Veronika Kadlicekova, Michal Kajsik, Katarina Soltys, Tomas Szemes, Livia Slobodnikova, Lucia Janosikova, Zuzana Hubenakova, Pauline Ogrodzki, Stephen Forsythe, Jan Turna, Hana Drahovska
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2018)
