Journal
JOURNAL OF FUNCTIONAL FOODS
Volume 5, Issue 3, Pages 1421-1431Publisher
ELSEVIER
DOI: 10.1016/j.jff.2013.05.011
Keywords
Angelica dahurica; Root; Coumarin; Acetylcholinesterase; Butylcholinesterase; Ultra performance liquid; chromatography
Categories
Funding
- Cooperative Research Program for Agriculture Science & Technology Development of Development of Functional Crop [PJ00925701]
- National Institute of Crop Science, Rural Development Administration, Republic of Korea
Ask authors/readers for more resources
The current research was the first to investigate coumarins and their cholinesterase inhibitory activities from Angelica dahurica roots. The ethanol extract of this species (100 mu g/ml) possessed inhibitory effects against human acetylcholinesterase (hAChE) and butylcholinesterase (BChE) with IC50 values of 26.40 and 14.71 mu g/ml, respectively. To evaluate the compounds responsible for these activities, the ethanol extract was chromatographed, which yielded ten coumarins, including isoimperatorin (1), imperatorin (2), senbyakangelicol (3), oxypeucedanin (4), byakangelicol (5), t-OMe-oxypeucedanin hydrate (6), t-OMe-byakangelicin (7), angelol H (8), byakangelicin (9), and oxypeucedanin hydrate (10). Among them, coumarin 5, 7, and 9 showed potent inhibition with IC(50)s of 46.3, 39.0, and 55.4 mu M (hAChE) as well as 45.2, 25.6, and 42.4 mu M (BChE), respectively. Moreover, their inhibition modes against two cholinesterases exhibited noncompetitive. The individual coumarin contents were remarkable differences, especially, oxypeucedanin (4) was the most predominant compound (8710.9 mu g/g), representing approximately 55.0% of the total content. (C) 2013 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available