Article
Chemistry, Multidisciplinary
Lenka Rysava, Milos Dvorak, Pavel Kuban
Summary: This study introduces a simple and convenient concept of automated blood sampling and analysis, allowing for accurate collection of blood samples and automated processing and analysis in a laboratory setting. Using warfarin blood concentration determination as an example, the concept demonstrates excellent analytical performance and wide applicability to various types of blood compounds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Marcus P. Wong, Michelle A. Meas, Cameron Adams, Samantha Hernandez, Valerie Green, Magelda Montoya, Brett M. Hirsch, Mary Horton, Hong L. Quach, Diana L. Quach, Xiaorong Shao, Indro Fedrigo, Alexandria Zermeno, Julia Huffaker, Raymond Montes, Alicia Madden, Sherri Cyrus, David McDowell, Phillip Williamson, Paul Contestable, Mars Stone, Josefina Coloma, Michael P. Busch, Lisa F. Barcellos, Eva Harris
Summary: Estimation of community-level antibody responses to SARS-CoV-2 from infection or vaccination is critical to inform public health responses. Traditional studies of antibodies rely on invasive blood collection, which is not suitable for pandemic-related social-distancing measures. Dried blood spots (DBS) are an alternative method that can be self-collected by participants at home and do not require refrigeration. In this study, we demonstrate that DBS are comparable to blood for measuring antibody responses to SARS-CoV-2 and can be used in longitudinal studies. Our work shows that DBS are an excellent sampling method when venipuncture is impractical.
MICROBIOLOGY SPECTRUM
(2022)
Article
Pharmacology & Pharmacy
Shin-Yi Lin, Yu-Fong Peng, Chih-Fen Huang, Ching-Hua Kuo, Sung-Chun Tang, Jiann-Shing Jeng
Summary: The study demonstrated that finger prick sampling with dried blood spot (fpDBS) cards in measuring dabigatran concentration is reliable and can be applied in clinical scenarios.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Obstetrics & Gynecology
Danielle Strom, Eve C. Feinberg
Summary: This series is a comprehensive review of the factors that influence the success of embryo transfer, including the impact of the performing physician, the use of adjunct therapies, the timing of the transfer, endometrial receptivity, and the health of the pregnancy and offspring.
FERTILITY AND STERILITY
(2022)
Review
Chemistry, Medicinal
Simone Donati, Francesca Marini, Francesca Giusti, Gaia Palmini, Cinzia Aurilia, Irene Falsetti, Teresa Iantomasi, Maria Luisa Brandi
Summary: Vitamin D deficiency is a global health problem with negative consequences on musculoskeletal and extra-skeletal health. Increasing intake of vitamin D fortified foods and administering vitamin D supplementation, such as vitamin D3 or calcifediol, can help improve vitamin D status. This review discusses the potential medical benefits and specific clinical scenarios in which oral intake of calcifediol could be effective in restoring optimal serum levels of 25(OH)D3. Insights into calcifediol-related non-genomic responses are provided, suggesting its use as a supplement for individuals at higher risk of hypovitaminosis D.
Article
Engineering, Electrical & Electronic
Md Saifuddin Faruk, Xiang Li, Derek Nesset, Ivan N. Cano, Albert Rafel, Seb J. Savory
Summary: Coherent optics is expected to be the logical choice for the physical layer in future passive optical networks, as intensity-modulation/ direct-detection technology will not meet the power budget requirements for high line-rate systems. The necessity, timeframe, and technological challenges of utilizing coherent technology in future passive optical networks are discussed in this article.
IEEE COMMUNICATIONS MAGAZINE
(2021)
Article
Biochemistry & Molecular Biology
Justyna Spiewak, Ivan Doykov, Apostolos Papandreou, Jenny Haellqvist, Philippa Mills, Peter T. Clayton, Paul Gissen, Kevin Mills, Wendy E. Heywood
Summary: Dried blood spot (DBS) biomarkers are convenient for monitoring specific lysosomal storage diseases (LSDs), but may also have relevance for other LSDs. A study was conducted to determine the specificity and utility of glycosphingolipidoses biomarkers in a DBS cohort of patients with various LSDs. The findings indicated that DBS glucosylceramide isoform profiling increased the specificity for the detection of Niemann-Pick disease type C (NPC), thereby improving diagnostic accuracy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Abhishek Jain, Montana Morris, Elizabeth Z. Lin, Sajid A. Khan, Xiaomei Ma, Nicole C. Deziel, Krystal J. Godri Pollitt, Caroline H. Johnson
Summary: Dried blood spot (DBS) metabolomics has many applications but faces challenges in accurate metabolite quantification due to the hematocrit effect and unknown blood-spotting volumes. This study compared five normalization methods and found that Hb normalization outperformed others in terms of intragroup variation, differential metabolic analysis, and classification accuracy. The study also demonstrated a correlation between specific gravity (SG) and Hb in adults and proposed an equation to use SG as a surrogate for Hb normalization. This comparative study provides a robust methodological platform for future environmental epidemiological studies.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Analytical
Martina Zanchetta, Bianca Posocco, Sara Gagno, Ariana Soledad Poetto, Marco Orleni, Giovanni Canil, Michela Guardascione, Fabio Puglisi, Giuseppe Toffoli
Summary: A new LC-MS/MS method was developed for quantifying LENVA in venous DBS samples, characterized by a short run time, volumetric sampling, and extraction of the entire spot to avoid Hct and spot volume effects. The method was validated on two different filter papers according to EMA, FDA, EBF, and IATDMCT guidelines, and showed satisfactory recovery, absence of matrix effect, process efficiency, Hct effect, linearity, precision, accuracy, selectivity, sensitivity, reproducibility, and stability. The method was successfully used to quantify LENVA in DBS samples from hepatocellular carcinoma patients and showed a good correlation with the standard procedure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Chih-Ning Cheng, Yu-Fong Peng, Ju-Yu Chen, Guan-Yuan Chen, Te- Weng, Ching-Hua Kuo
Summary: This study proposed an effective DBS preparation protocol to comprehensively evaluate the hematocrit (Hct) effect for accurate quantification of DBS samples. An LC-MS/MS method was used to quantify the target drugs in DBS samples, and the results showed the validity of the proposed protocol. This new protocol eliminated common pitfalls in studying the Hct effect and provided a comprehensive strategy for further DBS studies.
ANALYTICA CHIMICA ACTA
(2023)
Article
Health Care Sciences & Services
Evgenia Kotsifa, Chrysovalantis Vergadis, Michael Vailas, Nikolaos Machairas, Stylianos Kykalos, Christos Damaskos, Nikolaos Garmpis, Georgios D. Lianos, Dimitrios Schizas
Summary: HCC is the most common primary liver malignancy associated with liver cirrhosis and chronic liver disease. Key risk factors for HCC development include viral infections, alcoholic liver disease, and nonalcoholic fatty liver disease. Treatment options for HCC depend on tumor burden, liver function, and patient's health status, with surgical resection, transplantation, ablation, TACE, and systemic therapy being potential strategies.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Zachary C. Stensland, John C. Cambier, Mia J. Smith
Summary: Targeted therapies focusing on autoantigen-specific B cells have shown potential for more precise treatment of autoimmune disorders, while also presenting challenges that need to be addressed for their clinical applicability.
Article
Behavioral Sciences
Ruben Rial, Mourad Akaarir, Francesca Canellas, Pere Barcelo, Jose A. Rubino, Aida Martin-Reina, Antoni Gamundi, M. Cristina Nicolau
Summary: This report suggests that different species of animals have evolved different brain regions to support wakefulness, with fish using the spinal-rhombencephalic regions, amphibians and reptiles using the brainstem-diencephalic regions, and mammals developing the telencephalic cortex. Mammals also have a form of wakefulness known as REMS, which is highly adaptive for promoting maternal care in mammalian litter. However, REMS is not important for singleton animals and is maladaptive for marine mammals, leading to their loss of this feature. The wakeful states in animals prioritize immediate needs such as foraging, defense, and reproduction, while the recovery of allostatic loads occurs during the paralyzed form of wakefulness called NREMS in amphibians and reptiles. The regulation of REMS depends on a hypothalamic switch, while phasic REMS is controlled by an independent proportional pontine control.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Daniela Pereira, Diana Martins, Fernando Mendes
Summary: This review explores the use of immunotherapy in the treatment of head and neck cancer (HNC) patients. Immune checkpoint inhibitors (ICI) as monotherapy or in combination with chemotherapy show promising results. Therapeutic vaccines targeting the Human Papilloma Virus also increase the anti-tumor response. However, toxicity remains a challenge.
Article
Radiology, Nuclear Medicine & Medical Imaging
Alberto Stefano Tagliafico, Bianca Bignotti, Lorenzo Torri, Federica Rossi
Summary: Sarcopenia refers to the loss of skeletal muscle mass, which leads to a decline in physical performance. Radiology can play a crucial role in the early detection and treatment of sarcopenia. This article provides an update on the clinical knowledge of sarcopenia and discusses the advantages and limitations of radiological evaluation, including various body composition imaging modalities and controversial issues.
Article
Pharmacology & Pharmacy
Marissa F. Dockendorf, Gowri Murthy, Kevin P. Bateman, Prajakti A. Kothare, Melanie Anderson, Iris Xie, Jeffrey R. Sachs, Rubi Burlage, Andra Goldman, Matthew Moyer, Jyoti K. Shah, Rachel Ruba, Lisa Shipley, Jane Harrelson
CLINICAL PHARMACOLOGY & THERAPEUTICS
(2019)
Article
Biochemical Research Methods
Lisa A. Vasicek, Xin Zhu, Daniel S. Spellman, Kevin P. Bateman
Article
Chemistry, Multidisciplinary
Zhenwei Wei, Zhuoer Xie, Reshma Kuvelkar, Vinit Shah, Kevin Bateman, David G. McLaren, R. Graham Cooks
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Biochemical Research Methods
Christine Fandozzi, Christopher Evans, Amanda Wilson, Dian Su, Melanie Anderson, Valerie Clausen, Lieve Dillen, Fabio Garofolo, Chris Holliman, Elliott Nickbarg, Timothy Olah, Ragu Ramanathan, Hui Zhang, Surinder Kaur, Renuka Pillutla, Hongbin Yu, Kevin Bateman, Lorella Di Donato, Shawna Hengel, Wenying Jian, Barry Jones, John Kellie, Anita Lee, Joe Palandra, Natasha Savoie, Petia Shipkova, Susan Spitz, Dian Su, Matthew Szapacs, Jian Wang, Katherine Wright, Jianing Zeng
Article
Biochemical Research Methods
Surinder Kaur, Kevin P. Bateman, Jim Glick, Mark Jairaj, John F. Kellie, Jens Sydor, Jianing Zeng
Article
Biochemical Research Methods
Kevin P. Bateman
Article
Biochemical Research Methods
John F. Kellie, John C. Tran, Wenying Jian, Barry Jones, John T. Mehl, Ying Ge, Jack Henion, Kevin P. Bateman
Summary: Recent advancements in immunocapture methods and mass spectrometer technology have allowed intact protein mass spectrometry to be utilized for studying antibodies and other large biotherapeutics. While researchers have introduced methods for drug discovery and development stages, there is currently a lack of clear recommendations on best practices and limitations in data processing workflows depending on the vendor. This perspective shares experiences and recommendations for the current and future application of mass spectrometry in monitoring biotherapeutic molecules in preclinical and clinical studies.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Review
Biochemical Research Methods
Padmanabhan Eangoor, Sharmistha Das, Vincenzo Pucci
Article
Oncology
Samanthi A. Perera, Johnny E. Kopinja, Yanhong Ma, Eric S. Muise, Jason Laskey, Kalyan Chakravarthy, Yiping Chen, Long Cui, Jeremy Presland, Manjiri Sathe, Sarah Javaid, Ellen C. Minnihan, Heidi M. Ferguson, Jennifer Piesvaux, Bo-Sheng Pan, Shuxia Zhao, Sharad K. Sharma, Hyun Chong Woo, Vincenzo Pucci, Ian Knemeyer, Saso Cemerski, Jared Cumming, B. Wesley Trotter, Archie Tse, Anuradha Khilnani, Sheila Ranganath, Brian J. Long, David Jonathan Bennett, George H. Addona
Summary: STING agonists can enhance anti-PD-1 immune profile, promote tumor control, and have potential therapeutic value for patients with aggressive tumors.
MOLECULAR CANCER THERAPEUTICS
(2022)
Review
Pharmacology & Pharmacy
Simone Esposito, Laura Orsatti, Vincenzo Pucci
Summary: This review discusses the evolution of technology and strategy in elucidating peptide subcutaneous catabolism. Modern bioanalytical technologies and bioinformatics platforms have enabled in silico, in vitro, and in vivo tools for characterising peptide catabolism and guiding the design of peptides with improved subcutaneous bioavailability. More predictive models are desired to improve the correlation between subcutaneous catabolism and other factors driving subcutaneous absorption.
Article
Biochemical Research Methods
Adway O. Zacharias, Chang Liu, Zachary L. VanAernum, Thomas R. Covey, Kevin P. Bateman, Xiujuan Wen, David G. McLaren
Summary: The need for high-throughput intact protein analysis is increasing in drug discovery. Acoustic ejection mass spectrometry (AEMS) has been established as a fast analytical platform with high data quality. This study explores the application of AEMS in high-throughput intact protein analysis and demonstrates comparable data quality to LC-MS with significantly improved analysis speed.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Medicinal
Esther Torrente, Valentina Fodale, Alina Ciammaichella, Federica Ferrigno, Jesus M. Ontoria, Simona Ponzi, Ilaria Rossetti, Alessio Sferrazza, Jerome Amaudrut, Antonino Missineo, Simone Esposito, Simone Palombo, Martina Nibbio, Mauro Cerretani, Monica Bisbocci, Antonella Cellucci, Annalise di Marco, Cristina Alli, Vincenzo Pucci, Carlo Toniatti, Alessia Petrocchi
Summary: Protein tyrosine phosphatase SHP2 acts as an oncogenic protein by regulating various cytokine receptors and receptor tyrosine kinase signaling pathways. Here, we present a new series of SHP2 allosteric inhibitors with an imidazopyrazine 6,5-fused heterocyclic system as the central scaffold, which exhibit strong enzymatic and cellular potency. Compound 8, a highly potent SHP2 allosteric inhibitor, was identified through structure-activity relationship studies, showing novel stabilizing interactions compared to known SHP2 inhibitors. Further optimization led to the discovery of analogue 10, which demonstrates excellent potency and promising pharmacokinetic properties in rodents.
ACS MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Biochemical Research Methods
Wei Zeng, Kevin P. Bateman
Summary: Data from simulated Gaussian curves showed that 7 data points across a peak that is 9 seconds or less wide can accurately and precisely calculate the peak area for drug quantitation in drug discovery and development. There was no significant difference between different sampling intervals, peak widths, different days, different peak sizes, and different instruments.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Medicinal
Esther Torrente, Valentina Fodale, Alina Ciammaichella, Federica Ferrigno, Jesus M. Ontoria, Simona Ponzi, Ilaria Rossetti, Alessio Sferrazza, Jerome Amaudrut, Antonino Missineo, Simone Esposito, Simone Palombo, Martina Nibbio, Mauro Cerretani, Monica Bisbocci, Antonella Cellucci, Annalise di Marco, Cristina Alli, Vincenzo Pucci, Carlo Toniatti, Alessia Petrocchi
Summary: Protein tyrosine phosphatase SHP2 is an oncogenic protein that regulates multiple signaling pathways. This study identified a new series of SHP2 allosteric inhibitors with a central scaffold of imidazopyrazine 6,5-fused heterocyclic system, showing good potency in enzyme and cellular assays. Compound 8, a highly potent SHP2 allosteric inhibitor, was identified through SAR studies and X-ray analyses revealed novel stabilizing interactions. Further optimization led to the discovery of analogue 10, which exhibits excellent potency and promising pharmacokinetic profile in rodents.
ACS MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Oncology
Yun Wang, Long Cui, Peter Georgiev, Latika Singh, Yanyan Zheng, Ying Yu, Jeff Grein, Chunsheng Zhang, Eric S. Muise, David L. Sloman, Heidi Ferguson, Hongshi Yu, Cristina St. Pierre, Pranal J. Dakle, Vincenzo Pucci, James Baker, Andrey Loboda, Doug Linn, Christopher Brynczka, Doug Wilson, Brian B. Haines, Brian Long, Richard Wnek, Svetlana Sadekova, Michael Rosenzweig, Andrew Haidle, Yongxin Han, Sheila H. Ranganath
Summary: The study demonstrates that the EP4 inhibitor MF-766 synergizes with anti-PD-1 therapy to enhance anti-tumor activity by modulating infiltration profiles of myeloid cells, NK cells, cDCs, and T-cells in the tumor microenvironment (TME).