Article
Chemistry, Analytical
Eliana Carniel, Karen Adams dos Santos, Lucas de Andrade de Lima, Ramona Kohlrausch, Rafael Linden, Marina Venzon Antunes
Summary: This study developed and validated two methods for the determination of clozapine and norclozapine in dried blood spots and dried plasma spots. The results showed a strong correlation between the concentrations in dried samples and serum levels, with higher accuracy in dried plasma spots. This method can be used as an alternative sampling strategy in clozapine therapeutic drug monitoring.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Lenka Rysava, Milos Dvorak, Pavel Kuban
Summary: This study introduces a simple and convenient concept of automated blood sampling and analysis, allowing for accurate collection of blood samples and automated processing and analysis in a laboratory setting. Using warfarin blood concentration determination as an example, the concept demonstrates excellent analytical performance and wide applicability to various types of blood compounds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Medicinal
Yahdiana Harahap, Afaf Amma Lahilla, Anastasia Sharon Jautan, Amiral Hafidz, Sunarsih Sunarsih
Summary: This study analyzed the levels of acrylamide and glycidamide in dried blood spot samples of smokers and non-smokers. The results showed significantly higher levels of acrylamide and glycidamide in smokers compared to non-smokers. The study demonstrated that dried blood spots can be used to determine the levels of acrylamide and glycidamide in humans.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Chemistry, Medicinal
Ruqi Xi, Rahima Abdulla, Jiangyu Zhao, Haji Akber Aisa, Yongqiang Liu
Summary: The pharmacokinetic profile and metabolites of a compound called CAM106 were studied, which showed potent antiviral activity against influenza virus and produced multiple metabolites in rats. This study provided reliable data for further clinical research.
Article
Biochemistry & Molecular Biology
Alessia Cafaro, Federica Pigliasco, Sebastiano Barco, Federica Penco, Francesca Schena, Roberta Caorsi, Stefano Volpi, Gino Tripodi, Marco Gattorno, Giuliana Cangemi
Summary: Adenosine Deaminase 2 Deficiency (DADA2) is an autoinflammatory disease caused by mutations in the ADA2 gene. Timely diagnosis is crucial for starting effective treatments. A novel enzymatic assay method can accurately determine ADA2 enzyme activity, aiding in the diagnostic workflow of DADA2.
Article
Chemistry, Analytical
Martina Zanchetta, Bianca Posocco, Sara Gagno, Ariana Soledad Poetto, Marco Orleni, Giovanni Canil, Michela Guardascione, Fabio Puglisi, Giuseppe Toffoli
Summary: A new LC-MS/MS method was developed for quantifying LENVA in venous DBS samples, characterized by a short run time, volumetric sampling, and extraction of the entire spot to avoid Hct and spot volume effects. The method was validated on two different filter papers according to EMA, FDA, EBF, and IATDMCT guidelines, and showed satisfactory recovery, absence of matrix effect, process efficiency, Hct effect, linearity, precision, accuracy, selectivity, sensitivity, reproducibility, and stability. The method was successfully used to quantify LENVA in DBS samples from hepatocellular carcinoma patients and showed a good correlation with the standard procedure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Haiwei Cao, Yi Jiang, Shaomin Wang, Haihuan Cao, Yanyan Li, Jing Huang
Summary: Meropenem (MER) is a widely used drug for treating complicated and serious infections. Therapeutic drug monitoring (TDM) is a valid clinical tool to ensure adequate drug concentrations and avoid adverse reactions. This study developed a practical method using dried plasma spot (DPS) sampling for monitoring MER.
Article
Chemistry, Medicinal
Ruqi Xi, Rahima Abdulla, Miaomiao Zhang, Zhurakulov Sherzod, Vinogradova Valentina Ivanovna, Maidina Habasi, Yongqiang Liu
Summary: This study investigates the pharmacokinetic characterization and metabolites of tetrahydroisoquinoline compound BH. A UHPLC-MS/MS method was developed and validated to quantify BH in rat plasma, and its main metabolic pathways were identified. A total of 18 metabolites, including both phase I and phase II metabolites, were characterized. These findings provide guidance for future in-depth studies on BH.
Article
Infectious Diseases
Qian Liu, Lanyu Liu, Yu Yuan, Feifan Xie
Summary: This study developed a dried blood spots (DBS) method for pharmacokinetic studies in pediatric patients. The method used the Capitainer(R) qDBS device to collect a 10 mu L volume of blood on a paper card, and quantified the concentrations of eight antibiotics using UHPLC-MS/MS. The DBS method was shown to be simple, robust, and suitable for children with limited blood sampling.
Article
Chemistry, Analytical
Lisa Delahaye, Luc De Baerdemaeker, Christophe P. Stove
Summary: Paracetamol is the most commonly used analgesic and antipyretic worldwide, but its hepatotoxicity is the most common cause of acute liver failure in the Western world. To investigate individual differences in paracetamol metabolism and optimize dosing regimens, sensitive and rapid liquid chromatography-tandem mass spectrometry methods have been developed for quantifying paracetamol and its metabolites. These methods have been successfully validated and can be used for future pharmacokinetic studies.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Review
Chemistry, Analytical
Christina Johannsen, Ago Mrsa, Trine Gronhaug Halvorsen, Leon Reubsaet
Summary: This perspective explores the feasibility of utilizing dried blood spots and innovative approaches with cellulose filter paper for sample preparation in the determination of proteins and peptides using liquid chromatography coupled to mass spectrometry. The integration of sample preparation at an early stage of sample handling, such as starting protein digestion and affinity clean-up at the moment of sampling, is discussed for optimized time efficiency in clinical purposes.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Juan Le, Rui Peng, Shu-Lin Yang, Jian-Li Chen, Jing-Ru Cheng, Yan Li, Shao-Ting Wang
Summary: Dried blood spots (DBS) have been considered a promising alternative for therapeutic drug monitoring (TDM) of immunosuppressants (ISDs), but there are still several main issues. In this study, a new LC-MS/MS method was developed to quantify four ISDs from one piece of 3.2 mm DBS, demonstrating good throughput, applicability, reliability, and sensitivity. The method showed favorable interchangeability and accurate detection results when analyzing clinical samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Pharmacology & Pharmacy
Melanie Anderson, Marissa F. Dockendorf, Ian McIntosh, Iris Xie, Sheila Breidinger, Dongfang Meng, Sumei Ren, Wendy Zhong, Li Zhang, Brad Roadcap, Kevin P. Bateman, Julie Stone, Eric Woolf
Summary: This article discusses the challenges of employing new sampling techniques in large-scale clinical trials and the importance of anticipating and mitigating implementation risks.
Article
Biochemical Research Methods
Hanne Bendiksen Skogvold, Elise Mork Sandas, Anja Osteby, Camilla Lokken, Helge Rootwelt, Per Ola Ronning, Steven Ray Wilson, Katja Benedikte Presto Elgstoen
Summary: A single LC-MS method was developed for DBS metabolite analysis in clinical applications such as newborn screening, allowing for simultaneous analysis of a wide range of metabolites. The method utilized a diphenyl column, a multi-linear solvent gradient, and tailored MS settings to enhance sensitivity and reproducibility for diverse metabolites. The performance of the method was suitable for both untargeted and targeted approaches in clinically relevant experiments.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Robin Sunsong, Ting Du, Imoh Etim, Yun Zhang, Dong Liang, Song Gao
Summary: The study successfully developed a sensitive LC-MS-MS method to quantify polydatin and its metabolite, resveratrol, for pharmacokinetic study and to determine polydatin hydrolysis by microflora. The method showed good linearity, reproducibility, and stability of analytes in plasma, as well as efficient extraction recovery rates. The metabolic and PK studies revealed rapid hydrolysis of polydatin and exposure of both polydatin and resveratrol in plasma and tissues.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)