Article
Chemistry, Analytical
Thomas Meikopoulos, Olga Begou, Georgios Theodoridis, Helen Gika
Summary: A new method for quantitative analysis of four ceramide species in dried blood samples was developed using LC-MS/MS. The Capitainer device was utilized to provide accurate sample volumes, allowing for accurate determinations. The study showed the potential application of at-home blood microsampling for health monitoring.
ANALYTICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Juana Maria Gonzalez-Rubio, Noelia Dominguez-Morueco, Susana Pedraza-Diaz, Ana Canas Portilla, Miguel Angel Lucena, Ana Rodriguez, Argelia Castano, Marta Esteban-Lopez
Summary: Human exposure to mercury can have serious health effects, especially in vulnerable groups such as children and fetuses. A novel method using dried blood spot (DBS) samples to analyze total mercury has shown good results in terms of precision, accuracy, and recovery. This method can be an excellent alternative for assessing mercury exposure in vulnerable groups in clinical settings.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Lucia Paniagua-Gonzalez, Elena Lendoiro, Esteban Otero-Anton, Manuel Lopez-Rivadulla, Ana de-Castro-Rios, Angelines Cruz
Summary: This study aimed to perform a clinical validation for tacrolimus and mycophenolic acid in both DBS and MitraTM VAMS, fulfilling both analytical and clinical acceptance criteria. VAMS showed lower concentrations compared to routine matrices but met the analytical requirements after correction.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Miguel Gambell Barroso, Louise Gustafsson, Victoria Barclay, Camilla Linder
Summary: The aim of this study was to develop a nondestructive method to estimate hematocrit in dried blood spots. Standards and controls were created, and the DBS were scanned using a flatbed scanner. Gray values and pixel areas were analyzed with open-source software to estimate hematocrit and volume. The results were compared with hematological analyzer and showed high precision and accuracy.
Review
Medical Laboratory Technology
M. Thevis, Katja Walpurgis, A. Thomas
Summary: Blood testing plays a crucial role in anti-doping controls, and advancements in technology and sample collection systems have made microvolume blood samples more accessible and cost-effective. These samples have the potential to improve monitoring of hormone and drug levels, evaluate circulating drug concentrations in competition, and enhance stability in blood passport analyses and the detection of peptide hormones and steroid esters.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2023)
Article
Environmental Sciences
Jeremy P. Koelmel, Elizabeth Z. Lin, Emily Parry, Paul Stelben, Emma E. Rennie, Krystal J. Godri Pollitt
Summary: Non-targeted PFAS analysis was applied to human blood to understand the sources, concentrations, and toxicity of these compounds. High-resolution tandem mass spectrometry and software workflow were used for PFAS characterization in dried blood spots. A total of 28 PFAS and 86 potential PFAS were detected, providing insights for environmental epidemiology studies and policy-making.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biochemical Research Methods
Wenhao Qu, Xianglian Li, Guidi Tian, Linsheng Liu, Liyan Miao
Summary: In this study, a sensitive rapid assay for the simultaneous detection of warfarin enantiomers in mouse dried blood spot samples was developed and validated. The method showed high precision and reliability, and the samples remained stable at room temperature.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Biochemical Research Methods
Helena Beatriz Ferreira, Ines M. S. Guerra, Tania Melo, Hugo Rocha, Ana S. P. Moreira, Artur Paiva, M. Rosario Domingues
Summary: Dried blood spots (DBS) are being considered as an alternative method for blood collection in lipidomic and other omic analysis. While DBS are widely used in clinical settings for specific measurements, their application in lipidomic studies is still limited. The combination of DBS with lipidomic approaches has shown high sensitivity and specificity, making it a useful tool for biomarker identification.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yongtao Xiao, Ying Zhou, Kejun Zhou, Wei Cai
Summary: Using metabolomics profiling, this study identified potential biomarkers for biliary atresia in dried blood spots, providing a new approach for screening this condition.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Sigrid Deprez, Christophe P. Stove
Summary: In the field of solid organ transplantation, chemotherapy and autoimmune disorders, therapeutic drug monitoring (TDM) is necessary for immunosuppressant drugs due to their narrow therapeutic window and high variability. This review discusses the use of dried blood samples as an alternative for TDM, including pre-analytical considerations and implementation in clinical practice. The cost-effectiveness, availability of multiplexed methods, harmonization, and patient perception are also addressed. Future developments and obstacles for implementing dried blood-based TDM are discussed. & COPY; 2022 Elsevier B.V. All rights reserved.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Biochemical Research Methods
Marc Luginbuhl, Frederike Stoth, Alexandra Schrock, Stefan Gaugler, Wolfgang Weinmann
Summary: PEth, formed by enzymatic reaction between ethanol and phosphatidylcholine, is a direct marker for alcohol usage with a long elimination half-life. It has advantages over other alcohol markers but lacks standardized laboratory protocols. General guidelines for PEth analysis in dried blood spots are provided, including reference material evaluation and LC-MS/MS analysis, making it applicable for demographic studies and forensic applications.
Article
Endocrinology & Metabolism
Jesper Krogh, Peter Plomgaard, Ruth Frikke-Schmidt, Sten Velschow, Jesper Johannesen, Linda Maria Hilsted, Malene Schroder, Ulla Feldt-Rasmussen
Summary: This study assessed the performance and safety of Fluispotter for the first time in healthy subjects. Fluispotter is a novel body-worn device with automated extraction, collection, and storage of venous dried blood spot samples. The majority of participants were able to successfully collect all the samples, demonstrating that Fluispotter is safe and feasible.
ENDOCRINE CONNECTIONS
(2023)
Article
Biochemistry & Molecular Biology
Milena Rmandic, Ana Stajic, Jasna Jancic, Janko Samardzic, Nebojsa Jovic, Andelija Malenovic
Summary: A UHPLC-MS/MS method was developed and validated for the determination of zonisamide in DPS and DBS samples, showing its suitability for clinical application.
Article
Chemistry, Multidisciplinary
Ines Ait Belkacem, Noushine Mossadegh-keller, Penelope Bourgoin, Isabelle Arnoux, Marie Loosveld, Pierre-emmanuel Morange, Thibaut Markarian, Pierre Michelet, Jean Marc Busnel, Sandrine Roulland, Franck Galland, Fabrice Malergue
Summary: Blood cell analysis is crucial for biomedical research and healthcare, with dried blood spots offering a way to preserve cellular molecules. Optimized leucocyte elution techniques allow for analysis of recovered cells, and the new biomarker CD169 has the potential to differentiate patients.
Article
Pediatrics
Xu Xiaoyong, Wang Jinglin, Wang Guangfei, Zhang Huimin, Xu Hong, Li Zhiping
Summary: A microsampling TDM platform was developed and validated for the simultaneous quantification of vancomycin, meropenem, and linezolid. The platform showed excellent reliability and compatibility, making it suitable for real-time TDM-guided personalization of antimicrobial treatment in critically ill children.
FRONTIERS IN PEDIATRICS
(2023)