Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 3, Issue 21, Pages 4431-4438Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5tb00345h
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Funding
- Christian Doppler Research Association
- Austrian Ministry of Economics, Family and Youth (BMWFJ)
- Austrian Government
- State Government of Styria within COMET program
- State Government of Upper Austria within COMET program
- EPSRC [EP/I012060/1]
- Biotechnology and Biological Sciences Research Council (BBSRC UK) [BB/G021546/1]
- European Research Council [338895]
- Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center - U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-SC 0001035]
- BBSRC [BB/G021546/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/G021546/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I012060/1] Funding Source: researchfish
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Thiol-based chemistry provides a mild and versatile tool for surface functionalization. In the present work, mercaptosilane films were patterned by utilizing UV-induced photo-oxidation of the thiol to yield sulfonate groups via contact and interferometric lithography (IL). These photo-generated sulfonic acid groups were used for selective immobilization of amino-functionalized molecules after activation with triphenylphosphine ditriflate (TPPDF). Moreover, protein-resistant poly(oligoethyleneglycolmethacrylate) (POEGMA) brushes were grown from the intact thiol groups by a surface-induced polymerization reaction. Exploiting both reactions it is possible to couple amino-labelled nitrilotriacetic acid (NH2-NTA) to sulfonate-functionalized regions, enabling the site-specific binding of green fluorescent protein (GFP) to regions defined lithographically, while exploiting the protein-resistant character of POEGMA brushes to prevent non-specific protein adsorption to previously masked areas. The outstanding reactivity of thiol groups paves the way towards novel strategies for the fabrication of complex protein nanopatterns beyond thiol-ene chemistry.
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