Evaluation of antioxidant, in vitro cytotoxicity of micropropagated and naturally grown plants of Leptadenia reticulata (Retz.) Wight & Arn.-an endangered medicinal plant

Objective: To evaluate the antioxidant and anti proliferative potential of different solvent extract of micropropagated and naturally grown plants of Leptadenia reticulata against various cancer cell lines. Methods: In this study different extract were tested for cytotoxicity against human breast adenocarcinoma cell line MCF-7, human colon adenocarcinoma grade II cell line HT-29 and non cancer skeletal muscle cell line L6 through 3-(4, 5-dimethyl thiazol-2-yl)-5 diphenyl tetrazolium bromide assay. The total antioxidant potential was estimated by three different antioxidant model diphenylpicrylhydrazyl free radical scavenging activity. HA scavenging activity and Fed, reducing activity. Results: The ethyl acetate extract of both naturally grown plant and tissue cultured plant exhibited significant cytotoxieity with IC50 values of 21 mu g/mL, 26 mu g/mL and 22 mu g/mL; 20 mu g,/ mL, 30 mu g/mL and 18 mu g/mL respectively against three cell lines. The diphenylpicrylhydrazyl free radical scavenging activity was found to be highest with IC50, value of 267.13 mu g,/mL in ethyl acetate extract. The methanolic extract exhibited moderate antioxidant activity with IC50 of 510.15 mu g/mL. A highly positive correlation was observed between the antioxidant potential and cytotoxic activity of the plant. Conclusions: The strong cytotoxicity of ethyl acetate extract revealed anti carcinogenic potential of the plant which supports its traditional use as medicine. The present investigation is new to literature till date and will provide better scientific basis for future pharmacological, in vivo studies and novel source of pure bioactive compounds having anti cancer properties in this plant.