Article
Medicine, Research & Experimental
Xiao Li, Yuantao Gao, Feng Tian, Ruochen Du, Yitong Yuan, Pengfei Li, Fang Liu, Chunfang Wang
Summary: The overexpression of miR-31 in mouse NSCs may promote spinal cord injury repair, while the lack of miR-31 in zebrafish experiments blocks nervous system development.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Materials Science, Biomaterials
Jun Zhou, Yaqi Wu, Zhijian Tang, Kaipeng Zou, Juan Chen, Zuowei Lei, Xueyan Wan, Yanchao Liu, Huaqiu Zhang, Yu Wang, Armin Blesch, Ting Lei, Shengwen Liu
Summary: Studies have shown that alginate capillary hydrogels can fill the lesion cavity and promote axonal regeneration after grafting into the injured spinal cord. Neural stem/progenitor cells (NSPCs) can survive, proliferate, and differentiate into neurons within the capillaries in vitro. In animal experiments, a portion of the grafted cells can survive and differentiate into neurons, astrocytes, and oligodendrocytes. Furthermore, the grafted cells can promote the growth of host axons, form putative synapses with host neurons, improve electrophysiological conductivity, and partially restore locomotor function.
REGENERATIVE BIOMATERIALS
(2022)
Review
Cell Biology
Emily A. B. Gilbert, Nishanth Lakshman, Kylie S. K. Lau, Cindi M. Morshead
Summary: Spinal cord injury affects millions of people worldwide, and there is currently no cure. Utilizing endogenous neural stem cells (NSCs) to replace lost cells and promote structural repair is a promising approach. However, there are still many unanswered questions regarding the heterogeneity of NSCs, their interaction with the environment, and factors that enhance their response.
Article
Chemistry, Multidisciplinary
Min Hao, Lu Chen, Jianlong He, Xiaolei Zhao, He Xia, Xin Chen, Liyang Yu, Jichuan Qiu, Shiqing Feng, Yuanhua Sang, Hengxing Zhou, Hong Liu
Summary: In this study, 3D bioactive hydroxyapatite (HAp) nanobelt haystack-mouse NSC (mNSC) hybrid spheroids were customized to address the challenges of using neural stem cells (NSCs) in spinal cord injury (SCI). The specific nanobelt haystack framework provided hypoxia alleviation and neural differentiation promotion. The engineered bioresponsive 3D nanobelt haystack-mNSC hybrid spheroids effectively repaired SCI in vivo, demonstrating the potential of incorporating nanomaterials and cell-material interactions in stem cell therapy.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Medicine, Research & Experimental
Zhiwei Li, Ye Qi, Lei Sun, Zheng Li, Shaojuan Chen, Yuqi Zhang, Yuan Ma, Jinming Han, Zide Wang, Yulin Zhang, Huimin Geng, Bin Huang, Jian Wang, Gang Li, Xingang Li, Shaohua Wu, Shilei Ni
Summary: The 3D nanofibrous sponges developed in this study show promise for the treatment of spinal cord injury by promoting neuronal regeneration and fiber regrowth. In a rat SCI model, these scaffolds not only restored neural function but also facilitated axon reinnervation and remyelination.
Review
Medicine, Research & Experimental
Jincheng Li, Wenqi Luo, Chunsheng Xiao, Jianhui Zhao, Chunyu Xiang, Wanguo Liu, Rui Gu
Summary: Traumatic spinal cord injury (SCI) can cause severe neurological impairments. Current clinical treatments are limited, with unsatisfactory effects. Finding ways to recruit and facilitate the differentiation of endogenous neural stem/progenitor cells (eNSPCs) towards neurons is considered a promising strategy for the noninvasive and immune-compatible treatment of SCI. This manuscript discusses the responses of eNSPCs to exogenous interventions and various manipulation approaches to enhance neurogenesis and reconstruct the hostile microenvironment, including pharmacological treatments and regulation. Combined treatments are also highlighted for future investigations.
Article
Engineering, Biomedical
Yiwei Xu, Jing Zhou, Cuicui Liu, Sheng Zhang, Fenglin Gao, Wenjing Guo, Xiumin Sun, Chi Zhang, Heying Li, Zilong Rao, Shuai Qiu, Qingtang Zhu, Xiaolin Liu, Xiaodong Guo, Zengwu Shao, Ying Bai, Xiao Zhang, Daping Quan
Summary: The repair of spinal cord injury depends on remodeling the microenvironment and supporting the recruitment and differentiation of endogenous stem/progenitor cells. Decellularized tissue matrices have shown promise in promoting neural tissue regeneration, especially when derived from nervous system tissues. This study compared a DTM hydrogel derived from spinal cord (DSCM-gel) and one derived from peripheral nerves (DNM-gel), with results showing that DSCM-gel promotes NSPC viability, proliferation, migration, and differentiation into neurons in early 3D culturing stages.
Review
Neurosciences
Zhe-Lun Yang, Jian Rao, Fa-Bin Lin, Ze-Yan Liang, Xiong-Jie Xu, Yi-Ke Lin, Xin-Yao Chen, Chun-Hua Wang, Chun-Mei Chen
Summary: Spinal cord injury (SCI) has significant impacts on both patients' quality of life and their families. While cell therapy has made major breakthroughs in treating SCI, it also has disadvantages. In recent years, there has been growing interest in the potential therapeutic role of exosomes and exosomal ncRNAs in SCI treatment. They may be promising tools for treating spinal cord injury.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Cell & Tissue Engineering
Chi Xu, Wenxiang Fan, Ying Zhang, Horace H. Loh, Ping-Yee Law
Summary: This study reveals that OPRK1 agonists inhibit adult neurogenesis in the mouse hippocampus by upregulating the expression of miR-7a, which in turn downregulates Pax6/Neurog2/NeuroD1 activities and hinders neuronal differentiation of neural stem cells.
Article
Engineering, Biomedical
Bi-Qin Lai, Xiang Zeng, Wei-Tao Han, Ming-Tian Che, Ying Ding, Ge Li, Yuan-Shan Zeng
Summary: For millennia, adult mammals have been unable to recover function lost after severe spinal cord injury due to a failure of brain-derived nerve fiber regeneration across the lesion. Various potential approaches rely on neuronal relays to reconnect the segregated neural networks of the spinal cord, including the use of stem cell-derived neuronal relays and functional electrical stimulation.
Article
Cell Biology
Robert Chevreau, Hussein Ghazale, Chantal Ripoll, Chaima Chalfouh, Quentin Delarue, Anne Laure Hemonnot-Girard, Daria Mamaeva, Helene Hirbec, Bernard Rothhut, Shalaka Wahane, Florence Evelyne Perrin, Harun Najib Noristani, Nicolas Guerout, Jean Philippe Hugnot
Summary: Ependymal cells in the adult spinal cord activate multiple signaling pathways after injury, downregulate cilia-associated genes, upregulate various genes including Osmr, and the Osmr receptor Oncostatin may regulate the fate of ependymal cells towards astrocytic differentiation by affecting cell proliferation and differentiation.
Review
Cell Biology
Camila Marques de Freria, Erna Van Niekerk, Armin Blesch, Paul Lu
Summary: Spinal cord injury leads to irreversible functional impairment due to neuronal loss and disruption of connections, but neural stem cell therapy shows promising potential in promoting axonal regeneration and forming new connections. This therapy has implications for improving motor systems, including the corticospinal tract, and restoring sensory feedback in SCI patients.
Article
Cell Biology
Lourdes Rincon-Ortega, Andrea Valencia-Exposito, Anna Kabanova, Acaimo Gonzalez-Reyes, Maria D. Martin-Bermudo
Summary: Cell proliferation and differentiation have an inverse relationship, and the interaction between stem cells and the basement membrane (BM) plays a crucial role in regulating this process. Integrins, as the key mediators of cell-BM interactions, have been shown to control the balance between proliferation and differentiation in different types of stem cells. In this study, we found that eliminating integrins from follicle stem cells (FSCs) in the Drosophila ovary increases their proliferation capacity and leads to an excess of differentiated follicle cell types. Our results also suggest that integrins regulate proliferation by restraining the activity of the Notch/Delta pathway. This research enhances our understanding of cell-BM interactions in stem cells and has implications for their therapeutic potential.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Yu Li, Ping-Ping Shen, Bin Wang
Summary: Spinal cord injury presents a significant challenge in trauma repair due to its difficulty and increasing morbidity. Stem cell therapy, particularly induced pluripotent stem cells, holds great potential in treating spinal cord injury by differentiating into precursor cells of various neural cell types at the injury site.
NEURAL REGENERATION RESEARCH
(2021)
Article
Neurosciences
Xinyuan Hu, Zhong Liu, Xinru Zhou, Qian Jin, Wenrong Xu, Xiao Zhai, Qiang Fu, Hui Qian
Summary: This study investigated the therapeutic effects of human umbilical cord mesenchymal stem cell-derived small extracellular vesicles (hucMSC-sEVs) in spinal cord injury (SCI) treatment. The results showed that hucMSC-sEVs promoted functional recovery of the spinal cord by activating neural stem cells via the ERK1/2 pathway.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
