Article
Biology
Maren Kasper, Michael Heming, David Schafflick, Xiaolin Li, Tobias Lautwein, Melissa Meyer zu Horste, Dirk Bauer, Karoline Walscheid, Heinz Wiendl, Karin Loser, Arnd Heiligenhaus, Gerd Meyer zu Hoerste
Summary: Uveitis is a group of inflammatory eye diseases characterized by infiltration of leukocytes into the uveal tissues. HLA-B27-positive uveitis displays a unique pattern of ocular leukocyte infiltration with a prominent presence of plasmacytoid and classical dendritic cells. These cells play a central role in predicted local cell-cell communication within the eye, suggesting their importance in HLA-B27-associated inflammation.
Article
Immunology
Izabela Lenart, Linh-Huyen Truong, Dinh Dung Nguyen, Olga Rasiukiene, Edward Tsao, Jonathan Armstrong, Pankaj Kumar, Kirsty McHugh, Branca I. Pereira, Balraj S. Maan, Malgorzata A. Garstka, Paul Bowness, Neil Blake, Simon J. Powis, Keith Gould, Darren Nesbeth, Antony N. Antoniou
Summary: Peptide-loaded Major Histocompatibility Complex (pMHC) class I molecules can be expressed in a single chain trimeric (SCT) format. Certain MHC class I molecules, including HLA-B27 HC, tend to form aggregates. The folding and dimerization of the single chain complex are determined by specific regions of the HLA-B27 HC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yingyu Zhou, Tomohiro Takano, Yimei Wang, Xuyang Li, Rong Wang, Yoshio Wakatsuki, Haruyo Nakajima-Adachi, Masaru Tanokura, Takuya Miyakawa, Satoshi Hachimura
Summary: Beta-elemene has therapeutic effects on obesity-induced chronic inflammation by adjusting the balance of immune cell populations in fat tissue through the generation of regulatory T cells in the intestinal immune system by modulating DC function.
Review
Medicine, General & Internal
Shi Huan Tay, Joo Guan Yeo, Jing Yao Leong, Salvatore Albani, Thaschawee Arkachaisri
Summary: Juvenile spondyloarthritis (JSpA) is a diverse spectrum of immune-mediated inflammatory arthritides that typically onset in late childhood and adolescence. It is characterized by a strong association with HLA-B27, enthesitis, and bone resorption.
FRONTIERS IN MEDICINE
(2021)
Article
Rheumatology
Tri M. M. Tran, Tejpal Gill, Joshua Bennett, Sohee Hong, Vance Holt, Anders J. J. Lindstedt, Sufia Bakshi, Keith Sikora, Joel D. D. Taurog, Maxime Breban, Fatemeh Navid, Robert A. A. Colbert
Summary: This study aimed to investigate the functional basis for the epistasis between endoplasmic reticulum aminopeptidase 1 (ERAP1) and HLA-B27 in experimental spondyloarthritis (SpA). The results showed that ERAP1 deficiency increased the folded to unfolded ratio of HLA-B27 heavy chains and reduced HLA-B27 misfolding. Additionally, ERAP1 deficiency led to a reduction in endoplasmic reticulum stress and decreased the prevalence of arthritis in HLA-B27-Tg rats.
ARTHRITIS & RHEUMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Rohit Parthasarathy, Fernando Santiago, Peter McCluskey, Nadeem O. Kaakoush, Nicodemus Tedla, Denis Wake
Summary: Though the pathogenesis of HLA-B27-associated diseases such as AAU and AS is not well understood, evidence suggests a link to Gram-negative bacteria and subclinical bowel inflammation. The existing literature lacks adequate studies on causation and the role of viruses and fungi, and the unique disease model of AAU to study the gut microbiota has been neglected.
TRENDS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michael Hudec, Iva Jurickova, Kamila Riegerova, Saak Ovsepian, Marie Cerna, Valerie Brid O'Leary
Summary: This study reveals the genetic and extracellular exosomal effects of HLA transfer on T lymphocytes in the context of celiac disease (CeD), providing a deeper insight into this autoimmune disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medical Laboratory Technology
Mohammad-Ayman A. Safi
Summary: A cross-sectional study in Saudi Arabia revealed a 2.5% prevalence of HLA-B27 in the general population, with females showing a 1.5 times higher positivity rate than males. The study found no significant correlation between HLA-B27 and gender or blood groups.
CLINICAL LABORATORY
(2021)
Letter
Ophthalmology
Alessandro Marchese, Michele Cavalleri, Francesco Bandello, Giulio Modorati, Elisabetta Miserocchi
Summary: This case report describes the development and management of ocular decompression retinopathy after cataract surgery in a patient with chronic severe HLA-B27 associated anterior uveitis. Close monitoring of intraocular pressure and inflammation is necessary to prevent severe complications in these patients.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2022)
Article
Immunology
Farhad Babaie, Hamed Mohammadi, Sorayya Salimi, Alireza Ghanavatinegad, Mitra Abbasifard, Mehdi Yousefi, Mehrzad Hajaliloo, Younes Khalili, Sajjad Zamanlou, Roghaiyeh Safari, Maryam Hemmatzadeh, Alireza Rezaiemanesh, Reza Salimi, Behzad Baradaran, Zohreh Babaloo
Summary: This study aimed to evaluate the impact of Endoplasmic reticulum aminopeptidase 1 (ERAP1) polymorphisms on the expression of Human leukocyte antigen (HLA)-B27-free heavy chains (FHCs) on macrophages and subsequent NK cell activation in Ankylosing spondylitis (AS) patients. Results showed reduced expression of HLA-B27-FHCs on M1 and M2 macrophages in patients with protective ERAP1 genotype. Co-culturing ERAP1-inhibited M1 macrophages with NK cells from patients with protective genotype resulted in downregulation of CD69 and CD107a markers on NK cells and decreased number of IFN-gamma+ NK cells. Inhibiting ERAP1 activity to reduce NK cell activation may have therapeutic potential in treating AS patients.
CLINICAL IMMUNOLOGY
(2023)
Review
Immunology
Chengappa G. Kavadichanda, Jie Geng, Sree Nethra Bulusu, Vir Singh Negi, Malini Raghavan
Summary: The heritability of Spondyloarthritis (SpA) is emphasized by familial studies and its strong association with human leukocyte antigen (HLA)-B*27. In addition, non-HLA susceptibility loci, such as endoplasmic reticulum aminopeptidases (ERAP) and those related to the IL-23/IL-17 axes, have also been identified for SpA.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Haoming Luan, Tiffany Horng
Summary: Recent studies have shown that macrophage metabolism changes dynamically during microbial exposure, influencing a shift in inflammatory response. An initial increase in oxidative metabolism boosts the induction of inflammatory responses, while a shutdown of oxidative metabolism contributes to suppression of inflammatory responses.
CURRENT OPINION IN IMMUNOLOGY
(2021)
Article
Rheumatology
Shanshan Zhang, Hideaki Tsuji, Hui Jin, Koji Kitagori, Shuji Akizuki, Ran Nakashima, Hajime Yoshifuji, Masao Tanaka, Hisashi Arase, Koichiro Ohmura, Akio Morinobu
Summary: This study found that the heavy chain of IgG (IgGH) recognizes the RA-susceptible HLA class II molecule complex through RF. Various types of IgGH segments were synthesized and transfected into human embryonic kidney 293T cells to examine their surface expression and RF recognition. The results showed that the C(H)1 domain of IgGH specifically binds to RA-susceptible HLA-DR molecule and is expressed on the cell surface. RF specifically recognizes the C(H)3 domain of the IgGH/HLA-DR4 complex.
Article
Medicine, General & Internal
Aviran Aharon, Gil Benedek, Barhoum Barhoum, Elchanan Parnasa, Nur Magadle, Ofer Perzon, Dror Mevorach
Summary: Specific HLA alleles and motifs are associated with vaccine-induced myocarditis, indicating that immunogenetic factors in HLA peptide-binding grooves may influence the development of this condition.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Chemistry, Multidisciplinary
Kai Sun, Jinzhong Hu, Xiangyu Meng, Yunfeng Lei, Xuezhong Zhang, Zhuoxuan Lu, Liming Zhang, Zhifei Wang
Summary: Traditional chemo-immunotherapy can induce T cell immune response through immunogenic cell death (ICD), but the limited ICD hinders lasting antitumor immunotherapeutic efficacy. A novel tadpole-ovoid Au@HMnMSNs nanoreactor is constructed to generate intratumoral hydroxyl radicals for enhanced ICD induction and DC maturation, along with DOX and ASA for recruitment of immune cells and inhibition of immunosuppressive cells.
Article
Biochemistry & Molecular Biology
Carla Cunha, Graciosa Q. Teixeira, Claudia Ribeiro-Machado, Catarina L. Pereira, Joana R. Ferreira, Maria Molinos, Susana G. Santos, Mario A. Barbosa, Raquel M. Goncalves
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Engineering, Biomedical
Ana R. Almeida, Mafalda Bessa-Goncalves, Daniel M. Vasconcelos, Mario A. Barbosa, Susana G. Santos
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2020)
Article
Biochemistry & Molecular Biology
Carla Cunha, Catarina Leite Pereira, Joana R. Ferreira, Claudia Ribeiro-Machado, Sibylle Grad, Susana G. Santos, Raquel M. Goncalves
Summary: This study evaluated the therapeutic potential of a chemoattractant delivery system for intervertebral disc (IVD) and found that it has the ability to recruit more cells, but no evidence of IVD regeneration was observed. Systemic injection of mesenchymal stem cells (MSCs) may play a role in IVD regeneration to some extent, but no synergies were observed when combined with the chemoattractant delivery system. Further exploration is needed before successful clinical translation of IVD regeneration using this strategy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Andrew T. Meek, Nils M. Kronenberg, Andrew Morton, Philipp Liehm, Jan Murawski, Eleni Dalaka, Jonathan H. Booth, Simon J. Powis, Malte C. Gather
Summary: The authors introduce an interference-based method illuminated by two rapidly alternating wavelengths for real-time mapping of nanoscale forces with sub-second mechanical fluctuations. This technique allows monitoring of cellular forces with high throughput and excellent temporal resolution, providing a new approach for studying dynamic processes in mechanobiology.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Joao Paulo Bras, Isabelle Guillot de Suduiraut, Olivia Zanoletti, Silvia Monari, Mandy Meijer, Jocelyn Grosse, Mario Adolfo Barbosa, Susana Gomes Santos, Carmen Sandi, Maria Ines Almeida
Summary: This study suggests that male rats with high-CORT stress responsiveness exhibit increased anhedonia and passive coping responses in adulthood following peripubertal stress exposure. Additionally, exposure to peripubertal stress leads to increased hippocampal TNF-alpha expression, which correlates with passive coping responses. Furthermore, peripubertal stress has long-term effects on hippocampal microglia, particularly in high-CORT stress-responsive rats.
BRAIN BEHAVIOR AND IMMUNITY
(2022)
Article
Biochemistry & Molecular Biology
Caitlin Boyne, Debra Lennox, Olivia Beech, Simon J. Powis, Pankaj Kumar
Summary: The HLA-I system plays a crucial role in activating cytotoxic lymphocytes and immune attack against pathogen-infected and cancer cells. While the ligandome of cancer cell surface HLA class I molecules has been extensively studied, the ligandome of extracellular vesicles remains relatively undefined. Understanding this could advance immune-based therapeutic approaches for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Pankaj Kumar, Caitlin Boyne, Sydney Brown, Ayesha Qureshi, Peter Thorpe, Silvia A. Synowsky, Sally Shirran, Simon J. Powis
Summary: This study reveals the HLA-I ligandome in extracellular vesicles (EVs) from multiple cancer cell lines and identifies tumor-associated antigenic peptides. These findings contribute to a better understanding of tumor immune responses.
Article
Materials Science, Multidisciplinary
Silvana Azevedo, Raquel Costa-Almeida, Susana G. Santos, Fernao D. Magalhaes, Artur M. Pinto
Summary: Remarkable progress has been made in the field of nanomedicine and immunotherapy, particularly in the use of carbon nanomaterials for cancer treatment and immunomodulation. These materials offer various advantages and have the potential to revolutionize biomedical research. However, further improvements and translation to clinical applications are still required.
APPLIED MATERIALS TODAY
(2022)
Article
Immunology
Izabela Lenart, Linh-Huyen Truong, Dinh Dung Nguyen, Olga Rasiukiene, Edward Tsao, Jonathan Armstrong, Pankaj Kumar, Kirsty McHugh, Branca I. Pereira, Balraj S. Maan, Malgorzata A. Garstka, Paul Bowness, Neil Blake, Simon J. Powis, Keith Gould, Darren Nesbeth, Antony N. Antoniou
Summary: Peptide-loaded Major Histocompatibility Complex (pMHC) class I molecules can be expressed in a single chain trimeric (SCT) format. Certain MHC class I molecules, including HLA-B27 HC, tend to form aggregates. The folding and dimerization of the single chain complex are determined by specific regions of the HLA-B27 HC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Biomedical
M. Bessa-Goncalves, C. Ribeiro-Machado, M. Costa, C. C. Ribeiro, J. N. Barbosa, M. A. Barbosa, S. G. Santos
Summary: This study demonstrated that the incorporation of 10mM of magnesium sulfate improved the mechanical properties of the fibrinogen scaffold and promoted macrophage polarization to M2 phenotype. In vivo experiments showed that FgMg10 scaffolds reduced inflammatory mediators and increased M2 macrophage polarization.
ACTA BIOMATERIALIA
(2023)
Article
Immunology
Janos Kriston-Vizi, Izabela Lenart, Takao Iwawaki, Keith Gould, Darren Nesbeth, Simon J. Powis, Antony N. Antoniou
Summary: The study found that Salmonella infections induce and require unfolded protein response pathways for intracellular replication, and infections with Salmonella may be associated with the human leukocyte antigen HLA-B * 27:05. Expression of HLA-B * 27:05 alters the cellular distribution of Salmonella within epithelial cells, and is associated with the Salmonella's requirements for UPR induction and endoplasmic reticulum membrane expansion.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Carlota M. Relvas, Susana G. Santos, Maria J. Oliveira, Fernao D. Magalhaes, Artur M. Pinto
Summary: Skin cancer is a common and increasing type of cancer with two main categories: melanoma and non-melanoma. Current treatments, including surgery, radiation therapy, and chemotherapy, have limitations in terms of mortality and recurrence rates. Recent studies have focused on new approaches, such as immunotherapy and various forms of phototherapy. Among these, photoimmunotherapy has gained attention for its potential outcomes in combining photodynamic and/or photothermal therapy with a systemic immune response. This review critically discusses the properties and mechanisms of action of different nanomaterials in skin cancer photoimmunotherapy and presents important results in the field.
Article
Optics
Roopam K. Gupta, Nils Hempler, Graeme P. A. Malcolm, Kishan Dholakia, Simon J. Powis
Summary: T cells of the adaptive immune system are effectively protected against pathogenic challenges. Traditional labelling methods are time-consuming and expensive, but digital holographic microscopy with deep learning proves to be a faster and more cost-effective alternative. The combination of DHM and deep learning achieves high throughput and accuracy in classifying CD4+ and CD8+ T cell subsets.
Article
Materials Science, Biomaterials
Marco Araujo, Joao Silveira, Aureliana Sousa, Mafalda Bessa-Goncalves, Susana G. Santos, Cristina C. Barrias
Summary: The study introduced an innovative concept of a bioinspired alginate-melanin hybrid 3D scaffold for healing chronic intestinal wounds, addressing inflammation modulation and tissue regeneration. The scaffold demonstrated potential for reuse, remarkable cell colonization ability, and ECM-inspired design features, making it a promising candidate for clinical applications in chronic wound treatment.
BIOMATERIALS SCIENCE
(2021)