Article
Cell & Tissue Engineering
E. Salzer, T. C. Schmitz, V. H. M. Mouser, A. Vernengo, B. Gantenbein, J. U. Jansen, C. Neidlinger-Wilke, H-j. Wilke, S. Grad, C. L. Le Maitre, M. A. Tryfonidou, K. Ito
Summary: Strategies to regenerate or repair intervertebral discs are being explored, and ex vivo disc cultures are frequently used for studying human intervertebral disc degeneration (IVDD). A systematic search of induction methods for degenerative changes revealed five categories and 129 studies. Currently, spontaneously degenerated disc cultures from large animals provide the closest resemblance to human IVDD. Future advancements in disc culture setups may better mimic human IVDD.
EUROPEAN CELLS & MATERIALS
(2023)
Article
Oncology
Sian Chen, Chenbin Chen, Yuanbo Hu, Ce Zhu, Xiaozhi Luo, Lizhu Wang, Xiang Wang, Xiangwei Sun, Xiaodong Chen, Wangkai Xie, Han Lou, Xielin Huang, Chao Li, Jun Xu, Xiangyang Xue, Xian Shen
Summary: The study established a 3D culturing platform to accurately predict drug responses for gastric cancer. By evaluating cell proliferation, viability, and apoptosis markers, differential sensitivities to chemotherapeutics across different patient-derived tissues were identified, enabling personalized medication plans. This rapid, cost-effective, scalable, and reproducible protocol for GC tissue culture can greatly impact future oncological research.
FRONTIERS IN ONCOLOGY
(2021)
Review
Gastroenterology & Hepatology
Nayere Taebnia, Ute Romling, Volker M. Lauschke
Summary: Enteric bacterial infections are a major cause of disease and mortality worldwide, especially in developing countries. In vitro 2D monolayer cultures have provided valuable insights into the virulence mechanisms of various pathogens, leading to the discovery of potential targets for antimicrobial therapy and vaccines. More complex models, such as organoids, 3D bioengineered scaffolds, and microphysiological devices, have expanded our understanding of the effects of physiological and biological factors on infection. Advances in ex vivo cultures and intravital imaging have also allowed for the study of enteric pathogens on fluid secretion, barrier integrity, and immune cell surveillance. The combined use of these different methods promises to enhance our understanding of host-microbe interactions and their impact on intestinal health.
Review
Oncology
Daniel Ll Hughes, Aron Hughes, Zahir Soonawalla, Somnath Mukherjee, Eric O'Neill
Summary: This review evaluates dynamic physiological culture techniques in successful primary human tissue culture, with perfusion culture being the most common method. The median duration of successful primary human tissue culture was 8 days, with a wide range of 5 hours to 60 days noted. Combining dynamic culture with primary human tissue culture enhances long-term culture success rates and preservation of tissue functionality ex vivo.
Review
Chemistry, Multidisciplinary
Bohwa Han, Yeonju Song, Jeehun Park, Junsang Doh
Summary: This review discusses the use of nanomaterials to address the limitations of CAR-T cell therapy, including methods to improve expansion, gene delivery, and enhance antitumor functions. Understanding the biological principles behind various nanomaterials is crucial for their application in ex vivo engineering of T/NK cells.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Dentistry, Oral Surgery & Medicine
William Phillip Pereira Da Silva, Leonardo Alan Delanora, Barbara Ribeiro Rios, Maria Eloise de Sa Simon, Cortino Sukotjo, Leonardo P. Faverani
Summary: This study compared senile rats (SENIL) with young ovariectomized rats (OXV) to select a critical animal model for assessing bone repair with implant installation. The results showed that the SENIL group had lower cell viability, more critical gene expression, lower alkaline phosphatase activity, and mineralization nodules. In vivo histometric and biomechanical analysis also indicated inferior bone condition in the SENIL group. Confocal microscopy revealed fragile bones in the SENIL group. Microtomography results were similar between the groups. These findings suggest that the senile model can provide a more critical bone condition for investigating biomaterial properties and topographic changes.
JOURNAL OF APPLIED ORAL SCIENCE
(2023)
Article
Developmental Biology
Hessel Honkoop, Phong D. Nguyen, Veronique E. M. van der Velden, Katharina F. Sonnen, Jeroen Bakkers
Summary: In this study, a cardiac slice culture system was developed to enable real-time imaging of native cardiomyocyte dynamics, allowing for the discovery of cellular mechanisms during heart regeneration. This system provides a new research method for studying cardiomyocyte proliferation and allows for interventions with drugs.
Article
Biochemistry & Molecular Biology
Nickolas Chelyapov, Toai Nguyen, Rafael Gonzalez
Summary: Aging is a multifactorial process that involves senescence and the immune system. This study demonstrates that autologous propagated and activated NK cells can decrease senescent cells and immunosenescence in vitro, and reduce the aging-related inflammatory response based on marker analysis.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Materials Science, Biomaterials
Yijie Yu, Shihui Bian, Yu Jiang, Bo Li, Xinggang Cui, Shu Ding, Zhiyin Dai, Rui Chen, Wei Zhong, Wei Yuan
Summary: This study develops a new model for studying vascular cellular senescence by combining ex vivo aortic culture and Matrigel subcutaneous implantation. The results show that this model can accurately evaluate the degree of vascular aging and reveal differentially expressed genes and changes in inflammatory cytokines.
Article
Cell Biology
Jeremy Dufau, Joanne X. Shen, Morgane Couchet, Thais De Castro Barbosa, Niklas Mejhert, Lucas Massier, Elena Griseti, Etienne Mouisel, Ez-Zoubir Amri, Volker M. Lauschke, Mikael Ryden, Dominique Langin
Summary: Adipocytes play diverse roles in physiology and pathology, with white, beige, and brown adipocytes differing in their lipid handling and thermogenic capacity. Cultured differentiated preadipocyte cell lines and primary mature adipocytes are used to mimic different types of adipocytes, but challenges remain in culture conditions and limitations. Advances in three-dimensional culture, such as adipose spheroids, provide a more in vivo-like model for studying adipocyte function and morphology.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Satoshi Mizoguchi, Tomoshi Tsuchiya, Ryoichiro Doi, Tomohiro Obata, Mayumi Iwatake, Shintaro Hashimoto, Hirotaka Matsumoto, Hiroshi Yukawa, Hiroko Hayashi, Tao-Sheng Li, Kazuko Yamamoto, Keitaro Matsumoto, Takuro Miyazaki, Koichi Tomoshige, Takeshi Nagayasu
Summary: Two-dimensional cell cultures have played a significant role in lung cancer research, but there is increasing interest in 3D cultures as a more efficient and effective research model. This study successfully created an ex vivo lung cancer model based on bioengineered lungs, which closely replicated the 3D structure and tumor microenvironment of actual lungs. The results demonstrated the superiority of this model and its potential use in lung cancer research and pathophysiological studies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Cell Biology
Beate Guendel, Xinyuan Liu, Matthias Loehr, Rainer Heuchel
Summary: This review examines the current in vitro models for PDAC, emphasizing the challenges of late diagnosis and treatment resistance in improving patient outcomes. Researchers have established various in vitro and in vivo models for PDAC, with a growing focus on 3D cell culture models like spheroids and organoids.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ken Mizuta, Yoshitaka Katou, Baku Nakakita, Aoi Kishine, Yoshiaki Nosaka, Saki Saito, Chizuru Iwatani, Hideaki Tsuchiya, Ikuo Kawamoto, Masataka Nakaya, Tomoyuki Tsukiyama, Masahiro Nagano, Yoji Kojima, Tomonori Nakamura, Yukihiro Yabuta, Akihito Horie, Masaki Mandai, Hiroshi Ohta, Mitinori Saitou
Summary: This study demonstrates the ex vivo reconstitution of fetal oocyte development in humans and monkeys, providing a critical step forward for in vitro oogenesis in humans and uncovering important characteristics of fetal oocyte development in primates. The cytological and transcriptomic progressions of fetal oocyte development in vitro closely recapitulate those in vivo, revealing species-specific and conserved programs driving this process.
Article
Biochemistry & Molecular Biology
Yoichi Shida, Tsutomu Nakazawa, Ryosuke Matsuda, Takayuki Morimoto, Fumihiko Nishimura, Mitsutoshi Nakamura, Ryosuke Maeoka, Shuichi Yamada, Ichiro Nakagawa, Young-Soo Park, Motoaki Yasukawa, Takashi Tojo, Takahiro Tsujimura, Hiroyuki Nakase
Summary: GiNKs, as a promising cell-based immunotherapy, show potential in treating GBM by prolonging the survival of NOG mice carrying U87MG-derived tumors. Although PD-L1 is expressed on U87MG and T98G cells, the expression levels vary significantly. PD-1 blocking antibodies did not have an additional effect on prolonging survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Brendon Herring, Samuel Jang, Jason Whitt, Kayla Goliwas, Zviadi Aburjania, Vikas Dudeja, Bin Ren, Joel Berry, James Bibb, Andra Frost, Herbert Chen, John Bart Rose, Renata Jaskula-Sztul
Summary: This study proposes the use of an ex vivo 3D flow-perfusion bioreactor system for cultivating and studying patient-derived neuroendocrine tumors (NETs), demonstrating its utility and providing methods for evaluating therapeutic efficacy. The research suggests that the bioreactor system and similar 3D culture models may be valuable tools for culturing patient-derived NETs and monitoring their response to therapy ex vivo.
FRONTIERS IN ENDOCRINOLOGY
(2021)