Journal
VIRUSES-BASEL
Volume 10, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/v10090469
Keywords
geminivirus; nanovirus; satellite; RCA; RFLP; Circomics
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The experience with a diagnostic technology based on rolling circle amplification (RCA), restriction fragment length polymorphism (RFLP) analyses, and direct or deep sequencing (Circomics) over the past 15 years is surveyed for the plant infecting geminiviruses, nanoviruses and associated satellite DNAs, which have had increasing impact on agricultural and horticultural losses due to global transportation and recombination-aided diversification. Current state methods for quarantine measures are described to identify individual DNA components with great accuracy and to recognize the crucial role of the molecular viral population structure as an important factor for sustainable plant protection.
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