Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Jiaowei Wang, Wenguang Xie, Nan Li, Wenjuan Li, Zhishuai Zhang, Nana Fan, Zhen Ouyang, Yu Zhao, Chengdan Lai, Hao Li, Mengqi Chen, Longquan Quan, Yunpan Li, Yu Jiang, Wenqi Jia, Lixin Fu, Md. Abdul Mazid, Yanling Zhu, Patrick H. Maxwell, Guangjin Pan, Miguel A. Esteban, Zhen Dai, Liangxue Lai
Summary: Heterologous organ transplantation is limited by organ shortage. Generating human organs in large mammals through embryo complementation faces challenges, but using optimized culture conditions and overexpressing pro-survival genes has enabled the formation of organized human-pig chimeric kidney structures, offering new possibilities for regenerative medicine and the study of human kidney development.
Article
Biochemistry & Molecular Biology
Yang Yu, Xuechun Li, Yimei Li, Renyue Wei, Hai Li, Zhonghua Liu, Yu Zhang
Summary: This study establishes a stable iPSC-EC induction system and investigates the regulatory mechanism of endothelial differentiation. The derived piPSC-ECs form microvessel-like structures in vitro and promote blood vessel formation under pathological conditions. The activation of TGF-beta-Smad2/3 signaling promotes mesoderm formation and prevents endothelial differentiation in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Article
Developmental Biology
Masaki Kinoshita, Toshihiro Kobayashi, Benjamin Planells, Doris Klisch, Daniel Spindlow, Hideki Masaki, Susanne Bornelov, Giuliano Giuseppe Stirparo, Hitomi Matsunari, Ayuko Uchikura, Ismael Lamas-Toranzo, Jennifer Nichols, Hiromitsu Nakauchi, Hiroshi Nagashima, Ramiro Alberio, Austin Smith
Summary: This study successfully established pluripotent stem cell lines from porcine, ovine, and bovine embryos without using feeder cells or serum factors. The cells showed differentiation in teratomas and in vitro, with global transcriptome analyses revealing commonality in transcription factor expression across the three species. The research also demonstrated the generation of cloned porcine fetuses using genetically modified AFX stem cells through nuclear transfer.
Article
Veterinary Sciences
Yu-Ting Zhang, Wang Yao, Meng-Jia Chai, Wen-Jing Liu, Yan Liu, Zhong-Hua Liu, Xiao-Gang Weng
Summary: This study found that sow urine-derived cells can be used to produce SCNT embryos, with similar in vitro developmental ability to porcine embryonic fibroblasts (PEFs) and similar expression levels of core pluripotency factor and blastocyst cell apoptosis.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Cell Biology
Joohyeong Lee, Lian Cai, Mirae Kim, Hyerin Choi, Dongjin Oh, Ali Jawad, Eunsong Lee, Sang-Hwan Hyun
Summary: This study investigated the production of porcine tetraploid embryos using three different methods and found that there was no significant difference in the blastocyst development rate and tetraploid production rate among the three methods. However, embryos produced through the electro-fusion of two-cell stage parthenogenetic blastomere method had a lower percentage of apoptotic cells. Further analysis revealed that tetraploid embryos produced using this method had higher developmental competence and expression of trophectoderm cell marker genes. These high-quality blastocysts can be used as suitable source material for testing the differentiation potential of pluripotent stem cells in pigs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Tian Huang, Zhiwei Li, Peiru Lv, Jin Zhou, Chongyuan Ye, Aimin Li, Manman Yuan, Hongbo Liu, Gengsheng Cao
Summary: This study focuses on the impact of ovary acquisition season, embryo transfer season, and conditions of surrogate sows on cloning efficiency in pigs. The research found that cloning efficiency is highest in the spring and lowest in the autumn. Moreover, factors such as the number of recovered oocytes, rate of mature oocytes, and pregnancy rate of surrogate sows also varied significantly depending on the season.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
Seon Ah Ryu, Song Baek, Keun Cheon Kim, Eun Song Lee, Seung Tae Lee
Summary: The presence of cumulus cells is not essential for the completion of cytoplasmic maturation in oocytes after nuclear maturation. Removal of cumulus cells from porcine medium antral follicles (MAFs)-derived cumulus-oocyte complexes (COCs) after nuclear maturation results in significant improvements in cytoplasmic maturation and preimplantation development. The cumulus cells do not play a relevant role in cytoplasmic maturation of immature oocytes.
Article
Agriculture, Dairy & Animal Science
Chang Sin Rim, Yu Song Kim, Chol Ho Rim, Yong Je Ri, Ju Song Choe, Dae Song Kim, Gum Song Kim, Jung Il Ri, Ryu Chol Kim, Haide Chen, Lei Xiao, Zhixin Fu, Yong Jun Pak, Ui Myong Jong
Summary: The objective of this study was to evaluate the effect of roscovitine pretreatment on the number and developmental competence of matured oocytes for somatic cell nuclear transfer (SCNT). The study found that the pretreatment with roscovitine increased the number of matured oocytes per ovary, particularly in the small follicle group. Additionally, roscovitine pretreatment did not have any adverse effect on cloning efficiency.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Multidisciplinary Sciences
Lixia Zhao, Xuefei Gao, Yuxuan Zheng, Zixin Wang, Gaoping Zhao, Jie Ren, Jia Zhang, Jian Wu, Baojiang Wu, Yanglin Chen, Wei Sun, Yunxia Li, Jie Su, Yulin Ding, Yuan Gao, Moning Liu, Xiaochun Bai, Liangzhong Sun, Guifang Cao, Fuchou Tang, Siqin Bao, Pentao Liu, Xihe Li
Summary: The researchers have successfully established stem cells from porcine and human preimplantation embryos using the EPSC approach, enabling cross-species differentiation. Upon studying bovine EPSCs, they found that these stem cells express high levels of pluripotency genes in feeder-free culture and maintain genetic stability in long-term culture.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Reproductive Biology
Van Khanh Nguyen, Tamas Somfai, Daniel Salamone, Vu Thi Thu Huong, Huong Le Thi Nguyen, Quan Xuan Huu, Au Thi Hoang, Hieu Trung Phan, Yen Kim Thi Pham, Lan Doan Pham
Summary: An efficient culture system was established to produce embryos by SCNT of the endangered Vietnamese ? pig, achieving a blastocyst production rate of 26.4% by improving culture systems for donor cells, oocytes, and embryos culture. Transfer of embryos resulted in pregnancies, but live offspring were not obtained.
Article
Agriculture, Dairy & Animal Science
Tien Dat Nguyen, Honghui Li, Yiquan Zhuang, Bowei Chen, Keiji Kinoshita, Muhammad Ameen Jamal, Kaixiang Xu, Jianxiong Guo, Deling Jiao, Kumiko Tanabe, Yunfang Wei, Zhuo Li, Wenming Cheng, Yubo Qing, Hong-Ye Zhao, Hong-Jiang Wei
Summary: This study successfully established an Asian elephant's fibroblast cell lines and performed interspecies somatic cell nuclear transfer with porcine oocytes. The reconstructed embryos showed in vitro development but failed to develop in vivo. Further investigation is needed to understand the reasons behind the in vivo developmental failure.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Reproductive Biology
Jinying Zhang, Minglei Zhi, Dengfeng Gao, Qianqian Zhu, Jie Gao, Gaoxiang Zhu, Suying Cao, Jianyong Han
Summary: This review summarizes the progress on the derivation of porcine pluripotent stem cells (PSCs) and reprogrammed cells. It also elucidates the mechanisms of pluripotency changes during pig embryo development. The potential applications of stable porcine PSCs and the guidance they provide for future research direction are also discussed.
BIOLOGY OF REPRODUCTION
(2022)
Article
Cell Biology
Hideaki Kojima, Hiroshi Yagi, Hiroko Kushige, Yukiko Toda, Kazuo Takayama, Shinako Masuda, Toshinori Morisaku, Tomonori Tsuchida, Kohei Kuroda, Kazuya Hirukawa, Jumpei Inui, Kotaro Nishi, Yutaka Nakano, Masayuki Tanaka, Shutaro Hori, Yasushi Hasegawa, Yuta Abe, Minoru Kitago, Shungo Adachi, Masatoshi Tomi, Katsuhisa Matsuura, Hiroyuki Mizuguchi, Yuko Kitagawa
Summary: Human induced pluripotent stem cells (hiPSCs) are a promising cell source for studying disease pathology and treatment. In this study, hiPSC-derived cells were successfully seeded onto decellularized organ-derived scaffolds and evaluated in an in vivo-like environment.
Article
Cell Biology
Tianye Li, Xinyue Zhang, Linglin Cheng, Chunting Li, Zihan Wu, Yingqi Luo, Kunpeng Zhou, Yanlin Li, Qi Zhao, Yongye Huang
Summary: Resveratrol inhibits the survival of gastric tumor cells by promoting apoptosis, autophagy, and endoplasmic reticulum stress. It also alters the expression of several long non-coding RNAs, and knockdown of H19 enhances the therapeutic effect of resveratrol.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Virology
Lin Yang, Xiaohua Liu, Liying Zhang, Xue Li, Xinwei Zhang, Guyu Niu, Weilong Ji, Si Chen, Hongsheng Ouyang, Linzhu Ren
Summary: The study indicates that porcine TRIM21 plays a critical role in enhancing PCV2 infection by increasing interferons and proinflammatory factors while decreasing cellular apoptosis.
Review
Virology
Weijuan Jia, Si Chen, Shanshan Chi, Yunjiang He, Linzhu Ren, Xueli Wang
Summary: This paper reviews recent progress on tick-borne bacterial diseases, viral diseases, and parasitic diseases in China, emphasizing the significance of research on ticks and tick-borne diseases.
Article
Pharmacology & Pharmacy
Tianye Li, Zihan Tang, Chunting Li, Xiaoya Liu, Linglin Cheng, Zhijing Yang, Xiaojin Zhu, Weiwei Liu, Yongye Huang
Summary: This study found that co-treatment with cisplatin and magnesium chloride (MgCl2) reduced the survival rate and colony formation of bladder cancer cells. The combination therapy also increased the proportion of apoptotic cells. Magnesium was shown to moderately regulate the Wnt/β-catenin signaling pathway, enhancing the efficacy of cisplatin-based chemotherapy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xue Li, Si Chen, Guyu Niu, Xinwei Zhang, Weilong Ji, Ying Ren, Liying Zhang, Linzhu Ren
Summary: This study analyzed the genetic relationship between PCV4 and other circoviruses, and found that PCV4 is more similar to MiCV, suggesting a potential common origin or derivation from MiCV. This raises concerns about the potential threat of PCV4 to animals and even humans. Therefore, continuous monitoring of PCV4 infection and variation, as well as the establishment of prevention and treatment methods, is necessary.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xue Li, Si Chen, Liying Zhang, Guyu Niu, Xinwei Zhang, Lin Yang, Weilong Ji, Linzhu Ren
Summary: Coinfection of PCV2 and PRV can enhance the activation of immune and inflammatory pathways, promoting the expression of interferons and interleukins while inhibiting other immune pathways. These results contribute to understanding the immune escape mechanisms of PCV2 and PRV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Si Chen, Xue Li, Xinwei Zhang, Guyu Niu, Lin Yang, Weilong Ji, Liying Zhang, Linzhu Ren
Summary: This study found that PRV inhibits PCV2 proliferation at 36 to 72 hours post-infection, while PCV2 enhances PRV proliferation in the middle stage of infection. PRV is the main factor during coinfection. Coinfection with PCV2 and PRV activates the ERS pathway and upregulates the expression of related proteins, leading to increased cell apoptosis and exacerbation of diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Guyu Niu, Xinwei Zhang, Weilong Ji, Si Chen, Xue Li, Lin Yang, Liying Zhang, Hongsheng Ouyang, Chang Li, Linzhu Ren
Summary: In this study, PCV4 was successfully rescued and shown to be pathogenic in pigs. Visible pathological changes were observed in the inoculated piglets, and effective humoral and cellular immune responses were stimulated.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Oncology
Tianye Li, Zhenzhen Hao, Zihan Tang, Chunting Li, Linglin Cheng, Tao Wang, Xiaojin Zhu, Yunhao He, Yongye Huang, Bing Wang
Summary: BAP31 plays an important role in the migration of lung cancer cells, and its knockdown weakens cell migration. Treatment with TGF-beta can reduce cell migration and enhance proliferation, which is downregulated after BAP31 knockdown. Moreover, BAP31 regulates multiple signaling pathways, especially the Wnt signaling pathway.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiaoguang Pan, Kunli Qu, Hao Yuan, Xi Xiang, Christian Anthon, Liubov Pashkova, Xue Liang, Peng Han, Giulia Corsi, Fengping Xu, Ping Liu, Jiayan Zhong, Yan Zhou, Tao Ma, Hui Jiang, Junnian Liu, Jian Wang, Niels Jessen, Lars Bolund, Huanming Yang, Xun Xu, George M. Church, Jan Gorodkin, Lin Lin, Yonglun Luo
Summary: Evaluation methods for CRISPR RNA-guided nucleases off-targets, like SURRO-seq, are crucial for minimizing off-target effects and advancing gene therapies. The study highlights the impact of thermodynamically stable base pairs and free binding energy on RGN specificity.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Xue Li, Lin Yang, Si Chen, Jiawei Zheng, Huimin Zhang, Linzhu Ren
Summary: TRIM21, a member of the TRIM family, possesses an E3 ubiquitin ligase activity. Recent studies have shown that TRIM21 is involved in virus infection by interacting with viral proteins or modulating immune and inflammatory responses. TRIM21 also acts as a cytosol high-affinity antibody Fc receptor, triggering antibody-dependent intracellular neutralization (ADIN).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Virology
Hongsheng Ouyang, Linzhu Ren
Article
Cell Biology
Linglin Cheng, Shubo Li, Kailun He, Ye Kang, Tianye Li, Chunting Li, Yi Zhang, Wanlu Zhang, Yongye Huang
Summary: Melatonin, a hormone released from the pineal gland, inhibits gastric cancer cell migration and colony formation. By modifying signaling pathways, melatonin regulates migration and stemness in gastric cancer cells. Combination therapy with melatonin and cisplatin improves therapeutic efficacy.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Microbiology
Si Chen, Xue Li, Liying Zhang, Jiawei Zheng, Lin Yang, Guyu Niu, Huimin Zhang, Ying Ren, Jing Qian, Changjiang Sun, Linzhu Ren
Summary: This study analyzed 302 samples collected in the Jilin Province of China from 2016 to 2021 and investigated the genotypes and genetic characteristics of PCV2 isolates. The predominant genotypes of PCV2 in Jilin Province were found to be PCV2b, followed by PCV2e and PCV2d. Mutations were detected in the isolates, but no recombination occurred, indicating a stable genotype of PCV2 in Jilin Province. The study also highlighted changes in antigen epitopes and amino acid mutations compared to currently used vaccine strains.
Review
Biochemistry & Molecular Biology
Yuqi Wang, Linzhu Ren, Haocheng Bai, Qing Jin, Liying Zhang
Summary: Exosomes, extracellular vesicles (EVs) in bodily fluids, play important roles in various physiological processes. The interaction between autophagy and endosomal/exosomal pathways has significant influences on normal physiology and human diseases. The interplay between exosomes and the autophagy pathway is complex, with exosomes inducing intracellular autophagy and autophagy modulating the biogenesis and degradation of exosomes. They also contribute to the infection process of enveloped viruses by encapsulating viral components and transferring them between cells. This review provides valuable insights into the interplay between exosomes and autophagy, as well as their implications in enveloped virus infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
