Article
Plant Sciences
Chong Wang, Shuying Qu, Jie Zhang, Ming Fu, Xiaofei Chen, Wanqi Liang
Summary: Meiotic recombination begins with the formation of double-strand breaks in DNA, which is catalyzed by SPO11 and accessory proteins like MEI4/PRD2. In rice, the MEI4/PRD2 homolog OsPRD2 plays a crucial role in meiosis, primarily involved in the generation of chromosomes and assembly of the spindle. Although OsPRD2 does not participate in spindle assembly in rice, it plays a conserved role in the formation of double-strand breaks in DNA.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jodi D. Weiss, Shelby L. McVey, Sarah E. Stinebaugh, Caroline F. Sullivan, R. Kelly Dawe, Natalie J. Nannas
Summary: The assembly and disassembly of spindles in maize meiosis exhibit variability but follow a predictable series of events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lei Peng, Yijing He, Weihan Wang, Yajie Chu, Qixin Lin, Rong Rui, Qiao Li, Shiqiang Ju
Summary: In this study, the importance of PAK1 in regulating spindle assembly and cell cycle progression during the first meiotic division of porcine oocytes was highlighted. The activated PAK1 showed dynamic expression and its subcellular localization was closely associated with spindle dynamics. Inhibition of PAK1 activity by IPA-3 led to impaired extrusion of PB1 and failure of bipolar spindle formation, which could be restored by reducing disulfide bonds between PAK1 and IPA-3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biology
Nicolas Macaisne, Laura Bellutti, Kimberley Laband, Frances Edwards, Laras Pitayu-Nugroho, Alison Gervais, Thadshagine Ganeswaran, Helene Geoffroy, Gilliane Maton, Julie C. Canman, Benjamin Lacroix, Julien Dumont
Summary: During cell division, the microtubule-based spindle plays a crucial role in chromosome segregation. The interaction between spindle microtubules and kinetochores is essential for the proper alignment and segregation of chromosomes. This study focuses on a kinetochore module called the BHC module, which consists of BUB-1, HCP-1/2, and CLS-2, and shows that it stabilizes microtubules, promoting spindle formation and accurate chromosome segregation.
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.
Article
Cell Biology
Zhen-Nan Pan, Jing-Cai Liu, Jia-Qian Ju, Yue Wang, Shao-Chen Sun
Summary: This study demonstrates the critical roles of LRRK2 in mammalian oocyte meiosis, primarily through regulating actin assembly for spindle migration and maintaining mitochondrial function.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Cell Biology
Hong Yin, Teng Zhang, Hao Wang, Xin Hu, Xuan Hou, Xianbao Fang, Yaoxue Yin, Hui Li, Lanying Shi, You-Qiang Su
Summary: The research reveals that EML1 plays a crucial role in controlling oocyte meiotic progression in mice, and its loss leads to abnormal oocytes unable to progress to the second meiotic division.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aleksandar Mihajlovic, Candice Byers, Laura Reinholdt, Greg Fitzharris
Summary: Chromosome segregation errors in mammalian oocyte meiosis, including SAC ineffectiveness in meiosis-II, contribute to age-related aneuploidy.
Article
Reproductive Biology
Qingqing Cai, Keying Wen, Miao Ma, Wei Chen, Delin Mo, Zuyong He, Yaosheng Chen, Peiqing Cong
Summary: EZH2 plays a crucial role in porcine oocyte meiotic maturation by regulating spindle organization, maintaining chromosomal integrity, and mitochondrial function.
BIOLOGY OF REPRODUCTION
(2021)
Article
Plant Sciences
Wenqing Shi, Jianhui Ji, Zhihui Xue, Fanfan Zhang, Yongjie Miao, Han Yang, Ding Tang, Guijie Du, Yafei Li, Yi Shen, Zhukuan Cheng
Summary: PRD1 is involved in spindle assembly in rice meiosis, showing dynamic localization patterns during the leptotene stage and potentially promoting bipolar spindle assembly by modulating the orientation of sister kinetochores.
Article
Biology
Gabriel Cavin-Meza, Michelle M. Kwan, Sarah M. Wignall
Summary: Multiple motors work synchronously to establish and maintain the bipolarity of oocyte spindles in the absence of centrosomes.
Article
Reproductive Biology
Cecilia S. Blengini, Karen Schindler
Summary: This article discusses the basic mechanisms of spindle formation during meiotic maturation, focusing on studies in mice and humans. It also explores various factors that may influence spindle formation and stability, as well as the potential impact of assisted reproductive technologies on spindles and subsequent embryo development.
BIOLOGY OF REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Zhen-Yu She, Meng-Fei Xu, Sun-Ying Jiang, Ya-Lan Wei
Summary: This study reveals the role of CENP-E in regulating chromosome alignment and spindle organization in male spermatocyte meiotic division, contributing to faithful chromosome segregation and stability.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2022)
Article
Plant Sciences
Yazhong Wang, Yan Wang, Jie Zang, Huabang Chen, Yan He
Summary: The function of PRD1 in regulating meiotic DSB formation is conserved in maize, but its role in bipolar spindle assembly shows divergence among different plant species. ZmPRD1 is essential for DSB formation and homologous recombination in maize meiosis, but the function of PRD1 in bipolar spindle assembly during rice meiosis is not conserved in maize.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)