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Analysis of tissue inhibitor of metalloproteinase-2 gene polymorphisms in a Caucasian population with abdominal aortic aneurysms

ZENTRALBLATT FUR CHIRURGIE (2008)

Journal

ZENTRALBLATT FUR CHIRURGIE

Volume 133, Issue 4, Pages 332-337

Publisher

GEORG THIEME VERLAG KG

DOI: 10.1055/s-2008-1076862

Keywords

abdominal aortic aneurysm; polymorphism; genetics; TIMP-2

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Abstract

Background: The formation of sporadic abdominal aortic aneurysm (AAA) is explained by a remodelling of the extracellular matrix (ECM) and breakdown Of Structural components of the vascular wall. Matrix metalloproteinases are the principle matrix-degrading proteases and are known to play a major role in the remodelling of the extracellular matrix in arterial vessels. Their activity is controlled by tissue inhibitors of metalloproteinases (TIMPs). Decreased TIMP-1 and TIMP-2 expression in the extracellular matrix of the walls of AAAs has been demonstrated in several studies. This case-control study was designed to investigate the possible impact of genetic variants of the TIMP-2 gene in the aetiology of AAA and to reproduce a recently described significant difference in allele frequency of the SNP 303 (G) under bar >A in a German population. Methods: TIMP-2 single nucleotide polymorphisms (SNPs) were analysed in a Study sample of 50 patients with AAA and 41 controls. Differences in genotype and allele frequencies of the identified polymorphisms were determined after sequencing the entire coding region and selected parts of the promoter using the automated laser fluorescence technique. Results: Six polymorphisms were identified, one of which is described for the first time, located in the intron, (231+23 (C) under bar >T). An association of the SNP 303 (G) under bar >A with the phenotype was not confirmed in our study (p = 0.648). However, the CT genotype of the SN P -479 (C) under bar > T was more frequent in patients with AAA than in the control group (p=0.054). Conclusions: In our analysis of the TIMP-2 gene, we identified one new SNP. A previously published association of the SNP 303 (G) under bar >A with the phenotype could not be validated in our population. However, we detected an association for the CT genotype of one polymorphism in the promoter region (g-479 (C) under bar >T) and AAA. This result has to be proved in a second study sample.

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Pathogenesis and management of abdominal aortic aneurysm

Jonathan Golledge, Shivshankar Thanigaimani, Janet T. Powell, Phil S. Tsao

Summary: Abdominal aortic aneurysm (AAA) causes a high number of deaths worldwide every year, but no drug therapy has been proven to effectively limit AAA growth in randomized controlled trials. Genome-wide association studies have identified potential drug targets, and treatments to reduce low-density lipoprotein cholesterol and smoking cessation are also being considered. However, previous placebo-controlled randomized trials have not shown convincing evidence of drug efficacy, mainly due to limitations such as small sample sizes and poor participant retention. Further large prospective studies on other targets are needed to address this issue.

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