Article
Plant Sciences
Yuekai Su, Caicai Lin, Jin Zhang, Bei Hu, Jie Wang, Jingyu Li, Shiqi Wang, Ruihao Liu, Xia Li, Zhenqiao Song, Jianhua Wang
Summary: A novel one-step hairy root regeneration method was developed for Salvia miltiorrhiza, resulting in high tanshinone content in regenerated plants. This method provides a foundation for breeding S. miltiorrhiza and sustainable development of medicinal plant resources, serving as a useful reference for the application of other species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Bo-Cheng Yang, Meng-Shiou Lee, Ming-Kuem Lin, Wen-Te Chang
Summary: Recent studies have found strong connections between epigenetic modulation and secondary metabolites in plants. In this study, the effects of the DNA methylation inhibitor 5-azacytidine (5-Az) on secondary metabolite production, gene expression, and DNA methylation levels were investigated in the hairy roots of the medicinal plant Salvia miltiorrhiza. Results showed that the content of tanshinones, a type of secondary metabolite, increased by 1.5-5 times in the hairy roots treated with 5-Az. The methylation pattern in the promoter region of the gene encoding copalyl diphosphate synthase (CPS) was altered during 5-Az treatment, and several transcription factor binding sites were identified. This study provides evidence for a possible mechanism of DNA methylation in regulating tanshinone biosynthesis in the hairy roots of S. miltiorrhiza.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Huaiyu Deng, Qi Li, Ruizhi Cao, Yafei Ren, Guanfeng Wang, Hongbo Guo, Shuhai Bu, Jingying Liu, Pengda Ma
Summary: Soil salinity has a significant impact on the growth and development of Salvia miltiorrhiza, as well as seed germination. The study reveals that the transcription factor MYC2 plays a crucial role in plant stress resistance. Overexpression of SmMYC2 enhances salt tolerance in transgenic plants by regulating the expression of antioxidant enzymes, proline, and flavonoid synthesis genes.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Meiling Ding, Bin Zhang, Shuo Zhang, RongRong Hao, Yu Xia, Pengda Ma, Juane Dong
Summary: Phenolic acids are the main bioactive compounds in Salvia miltiorrhiza, and their production can be increased by salicylic acid (SA) treatment. In this study, a negative regulator of SA-induced phenolic acid biosynthesis, SmNPR4, was identified in S. miltiorrhiza hairy roots. This study also discovered a transcription factor, SmTGA5, that interacts with SmNPR4 and activates the expression of the phenolic acid biosynthesis gene SmTAT1. The results showed that SA can alleviate the inhibitory effect of SmNPR4 on SmTGA5. These findings enhance our understanding of the molecular mechanism behind salicylic acid regulation of phenolic acid biosynthesis in S. miltiorrhiza.
HORTICULTURE RESEARCH
(2023)
Article
Agricultural Engineering
Bingcong Xing, Jiaxiu Sun, Haizheng Yu, Xuemin Zhang, Kai Fan, Zongsuo Liang
Summary: This study identified a novel transcription factor, SmWD40-2, in Salvia miltiorrhiza, which plays an important role in the biosynthesis of tanshinones and phenolic acids. Overexpression of SmWD40-2 increased the content of bioactive compounds, while silencing SmWD40-2 reduced the content of bioactive compounds.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Ronghui Tan, Min Chen, Lu Wang, Jinjia Zhang, Shujuan Zhao
Summary: Overexpression and antisense expression of Sm4CL2 redirected the biosynthesis of salvianolic acids and tanshinones in Salvia miltiorrhiza hairy roots. Transcriptomic analysis revealed that overexpression of Sm4CL2 caused the upregulation of other phenylpropanoid pathway genes and antisense Sm4CL2 expression resulted in the downregulation of other phenylpropanoid pathway genes but activated the expression of terpenoid pathway genes. Protein-protein interaction analysis suggested that Sm4CL2 might interact with PAL, PAL4, CSE, CCoAOMT and SmCYP84A60, and played a key role in the interaction network. The tracking work in this study proved that Sm4CL2 could redirect both salvianolic acids and tanshinones biosynthesis possibly through synergistically regulating other pathway genes.
PLANT CELL REPORTS
(2023)
Article
Plant Sciences
Wanying Duan, Hui Sun, Weina Ding, Jie Zhou, Lei Fang, Lanping Guo
Summary: The effect of karrikinolide (KAR1) on the biosynthesis of salvianolic acid in hairy roots of Salvia miltiorrhiza was investigated using transcriptomics technology. Transcriptome analysis revealed significant differences in the response of S. miltiorrhiza to KAR1 treatment. Genes related to salvianolic acid biosynthesis were up-regulated after KAR1 treatment. These findings provide insights into the molecular mechanism of salvianolic acid biosynthesis and serve as a reference for genetics research.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Romeo Arago Dougue Kentsop, Valeria Iobbi, Giuliana Donadio, Barbara Ruffoni, Nunziatina De Tommasi, Angela Bisio
Summary: Salvia corrugata hairy roots were obtained through transformation, serving as a potential source of abietane and abeo-abietane compounds with antibacterial, antitumor, and cytotoxic activities. The study demonstrated the presence of horminone, ferruginol, 7-O-acetylhorminone, and 7-O-methylhorminone in untransformed roots, while agastol and ferruginol were isolated and quantified from the hairy roots, indicating their potential as bioactive compound sources.
Article
Plant Sciences
Wei Zhou, Shuai Wang, Yafang Shen, Yunhui Liu, Itay Maoz, Xiankui Gao, Chengan Chen, Tingyao Liu, Can Wang, Guoyin Kai
Summary: In this study, a YE-responsive transcriptional factor SmSCR1 was identified in Salvia miltiorrhiza, which can enhance tanshinone accumulation and affect the growth and development of hairy roots. Furthermore, it was found that SmSCR1 can bind to the promoter of SmCPS1 to induce its expression.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Caicai Lin, Lin Zhang, Xia Zhang, Xin Wang, Chaoyang Wang, Yufeng Zhang, Jianhua Wang, Xingfeng Li, Zhenqiao Song
Summary: This study investigated the early root development of Salvia miltiorrhiza and the synthesis of tanshinones, providing new insights into the biosynthesis process. Three critical stages of tanshinone synthesis were identified, with most genes up-regulated during the formation of the red taproot. Plant hormone signal transduction and ABC transport pathways were found to play important roles in the taproot development, and potential candidate genes involved in tanshinone synthesis were identified for further investigation. The results lay a necessary foundation for analyzing the initial synthesis and regulation mechanism of Tanshinones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Jingyin Kong, Songjun Li, Yingting Li, Min Chen
Summary: Placenta-mediated pregnancy complications (PMPCs), such as preeclampsia (PE), fetal growth restriction (FGR), and recurrent spontaneous abortion (RSA), occur in approximately 5% of pregnancies and are caused by abnormal placenta development. Synthetic drugs have limitations in treating PMPCs due to their adverse effects. Salvia miltiorrhiza (S. miltiorrhiza) shows potential in treating PMPCs through its cardiovascular benefits, including anticoagulation, vasodilation, antioxidation, and endothelial protection. This review provides comprehensive insights on S. miltiorrhiza, its phytochemical constituents, pharmacological activities, and PMPCs, which could contribute to the development of promising drugs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Medicinal
Jian-Shuang Jiang, Quan-chang Gu, Zi-Ming Feng, Xiang Yuan, Xu Zhang, Pei-Cheng Zhang, Ya-Nan Yang
Summary: Seven new phenolic acids were isolated from the roots of Salvia miltiorrhiza. Compound 4 exhibited a moderate neuroprotective activity.
Article
Biochemistry & Molecular Biology
Jian-Shuang Jiang, Quan-chang Gu, Zi-Ming Feng, Xu Zhang, Pei-Cheng Zhang, Ya-Nan Yang
Summary: Six undescribed tanshinones were isolated from the rhizome of Salvia miltiorrhiza Bunge. They exhibited good antioxidant activities in cardiomyocytes and showed potential in protecting the cells.
Article
Pharmacology & Pharmacy
Jun-Lan Lu, Xue-Shan Zeng, Xin Zhou, Jun-Ling Yang, Ling-Ling Ren, Xin-Yu Long, Feng-Qing Wang, Olajide E. Olaleye, Nan-Nan Tian, Ya-Xuan Zhu, Jia-Jia Dong, Wei-Wei Jia, Chuan Li
Summary: This study investigated the molecular basis of hepatobiliary and renal excretion of Danshen compounds and found several human and rat transporters that mediate the uptake of these compounds. These findings contribute to a better understanding of the metabolism of Danshen phenolic acids and can facilitate pharmacokinetic research on other Danshen-containing injections.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Haimin Chen, Jialing Chen, Yao Qi, Siyuan Chu, Yao Ma, Linna Xu, Shiyi Lv, Haihua Zhang, Dongfeng Yang, Yonghong Zhu, Dennis Ra Mans, Zongsuo Liang
Summary: Salvia miltiorrhiza is a traditional medicinal plant used for cardiovascular and cerebrovascular disease, with tanshinones being its main bioactive constituents. The endophytic fungi Cladosporium tenuissimum DF11 can promote tanshinone biosynthesis and accumulation in S. miltiorrhiza roots, indicating its potential as a biofertilizer fungus to regulate and stabilize the quality of cultivated medicinal materials.
