Article
Plant Sciences
Yanyan Yan, Qinghua Shi, Biao Gong
Summary: GSNOR negatively regulates NO homeostasis during tomato axillary bud outgrowth, promoting bud growth by affecting auxin and cytokinin signaling, leading to a decreased auxin:cytokinin ratio and breaking bud dormancy.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Megan Pophal, Zachary W. Grimmett, Clara Chu, Seunghee Margevicius, Thomas Raffay, Kristie Ross, Anjum Jafri, Olivia Giddings, Jonathan S. Stamler, Benjamin Gaston, James D. Reynolds
Summary: The study suggests that F-ENO could reflect airway S-nitrosothiol concentrations and serve as a biomarker for asthma. Inhalational challenge with GSNO resulted in increased F-ENO, indicating a potential non-invasive test for monitoring airway GSNO breakdown.
Article
Chemistry, Multidisciplinary
Zijie Luo, Yingzhu Zhou, Tao Yang, Yuan Gao, Priyank Kumar, Rona Chandrawati
Summary: Ceria nanoparticles can induce the generation of nitric oxide in addition to scavenging it. This study demonstrates that ceria nanoparticles catalytically decompose S-nitrosoglutathione to produce nitric oxide, with a high release recovery rate and maintained crystalline structure. The newly discovered capability of ceria nanoparticles to generate nitric oxide from GSNO overturns the conventional concept of their role solely as NO-scavenging agents, and shows potential therapeutic effects in cancer cell suppression.
Article
Plant Sciences
Lin Zhang, Haiyan Song, Baohai Li, Meng Wang, Dongwei Di, Xianyong Lin, Herbert J. Kronzucker, Weiming Shi, Guangjie Li
Summary: High levels of NH4+ induce NO accumulation and inhibit K+ acquisition via SNO1/SOS4, leading to reduced root growth tolerance, while GSNOR plays a crucial role in improving root tolerance to NH4+ by counteracting NO-mediated suppression of tissue K+.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Medicine, Research & Experimental
Narayani Nagarajan, Shin-ichi Oka, Jihoon Nah, Changgong Wu, Peiyong Zhai, Risa Mukai, Xiaoyong Xu, Sanchita Kashyap, Chun-Yang Huang, Eun-Ah Sung, Wataru Mizushima, Allen Sam Titus, Koichiro Takayama, Youssef Mourad, Jamie Francisco, Tong Liu, Tong Chen, Hong Li, Junichi Sadoshima
Summary: Modification of cysteine residues by oxidative and nitrosative stress affects protein structure and function, contributing to cardiovascular disease. Thioredoxin 1 (Trx1) acts as a denitrosylase or transnitrosylase, and in this study, it was found to transnitrosylate Atg7, promoting autophagy. During ischemia, Trx1 was oxidized and S-nitrosylated, and Atg7 reduced the disulfide bond in Trx1, allowing NO to be released and transferred to Atg7. Experiments with Atg7 C402S-knockin mice showed that S-nitrosylation of Atg7 at Cys402 promotes autophagy, protecting the heart against ischemia.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Eva Esther Rudolf, Patrick Huether, Ignasi Forne, Elisabeth Georgii, Yongtao Han, Ruediger Hell, Markus Wirtz, Axel Imhof, Claude Becker, Joerg Durner, Christian Lindermayr
Summary: The study reveals that GSNO reductase-deficient plants accumulate SAM, leading to increased methylation activity and significant changes in histone methylation status, which in turn affect chromatin accessibility and enhance DNA methylation.
Review
Cell Biology
Meret Sarah Allemann, Pratintip Lee, Jurg H. Beer, Seyed Soheil Saeedi Saravi
Summary: Cardiovascular aging poses a significant challenge and requires innovative strategies to restore aging cardiovascular tissues. Targeting the redox machinery holds promise to optimize cardiovascular regenerative therapies by enhancing stem cell function and rejuvenating cardiovascular tissues.
Article
Plant Sciences
Patrick Treffon, Jacopo Rossi, Giuseppe Gabellini, Paolo Trost, Mirko Zaffagnini, Elizabeth Vierling
Summary: Nitric oxide (NO) is a crucial signaling molecule in plants, regulated by the formation of S-nitrosoglutathione (GSNO) and the activity of the enzyme S-nitrosoglutathione reductase (GSNOR). Proteomic analysis of an Arabidopsis thaliana GSNOR null mutant revealed changes in proteins associated with chlorophyll metabolism and redox stress, as well as a significant increase in proteins from the aldo-keto reductase (AKR) protein superfamily. The study identified NADPH-dependent activity in GSNO reduction in plants, adding a new component to NO metabolism in plant cells.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Medicine, Research & Experimental
Nolan T. Carew, Heidi M. Schmidt, Shuai Yuan, Joseph C. Galley, Robert Hall, Helene M. Altmann, Scott A. Hahn, Megan P. Miller, Katherine C. Wood, Bethann Gabris, Margaret C. Stapleton, Sean Hartwick, Marco Fazzari, Yijen L. Wu, Mohamed Trebak, Brett A. Kaufman, Charles F. McTiernan, Francisco J. Schopfer, Placido Navas, Patrick H. Thibodeau, Dennis M. McNamara, Guy Salama, Adam C. Straub
Summary: This study reveals the crucial role of cytochrome b5 reductase 3 (CYB5R3) in cardiomyocyte redox biology and identifies a common genetic variant that may increase the risk of death in Black patients with heart failure.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Judong Kim, S. M. Touhidul Islam, Fei Qiao, Avtar K. Singh, Mushfiquddin Khan, Jeseong Won, Inderjit Singh
Summary: The research shows that regulating the cytokines released by B cells can protect against neuroinflammatory diseases like experimental autoimmune encephalomyelitis. Using GSNO and GSNOR inhibitor N6022 can modulate IL-10 and IL-6, offering potential in treating autoimmune disorders.
Article
Plant Sciences
Dora Olah, Selahattin Kondak, Arpad Molnar, Oluwatosin Peace Adedokun, Zalan Czekus, Katalin Gemes, Gabor Galbacs, Zsuzsanna Kolbert
Summary: This study found that limited zinc supply affects the gene and protein levels of GSNOR enzyme, but not its activity, and modifies reactive species levels and physiological nitroproteome in Arabidopsis.
Article
Chemistry, Applied
Xiaozhao Wang, Jixuan Cao, Jin Qiao, Jing Pan, Song Zhang, Qingqing Li, Qingguo Wang, Biao Gong, Jingying Shi
Summary: This study found that treating cherry tomatoes with GABA can enhance their disease resistance by regulating GSNOR to maintain endogenous NO balance.
Article
Multidisciplinary Sciences
SeungHye Han, Minho Lee, Youngjin Shin, Regina Giovanni, Ram P. Chakrabarty, Mariana M. Herrerias, Laura A. Dada, Annette S. Flozak, Paul A. Reyfman, Basil Khuder, Colleen R. Reczek, Lin Gao, Jose Lopez-Barneo, Cara J. Gottardi, G. R. Scott Budinger, Navdeep S. Chandel
Summary: Alveolar epithelial type 1 (AT1) cells are necessary for gas exchange, while alveolar epithelial type 2 (AT2) cells play a role in lung development and repair. In this study, deleting a specific subunit of the mitochondrial electron transport chain complex I in lung epithelial cells led to abnormal alveolar development and death. Restoring NAD(+) regeneration was able to correct the developmental defects and prevent lethality. These findings highlight the importance of mitochondrial complex I-dependent NAD(+) regeneration in directing cell fate during lung development.
Review
Cardiac & Cardiovascular Systems
Andrew J. Smith
Summary: With the increasing research and understanding of the cardiotoxic side-effects of anticancer therapy, it is crucial to consider the range of myocardial cell types affected. The role of cardiac stem/progenitor cells in myocardial repair and renewal is significant, but their functions may be limited and worsened by cardiotoxins, impacting long-term prognosis of patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Colin T. Stomberski, Nicholas M. Venetos, Hua-Lin Zhou, Zhaoxia Qian, Bryce R. Collison, Seth J. Field, Richard T. Premont, Jonathan S. Stamier
Summary: Accumulating evidence indicates that protein S-nitrosylation is regulated by enzymes, and specificity in S-nitrosylation is derived from dedicated S-nitrosylases and denitrosylases. In this study, mice deficient in the protein denitrosylase SCoR2 showed reduced serum cholesterol due to decreased secretion of the cholesterol-regulating protein PCSK9. The researchers found that SCoR2 controls an S-nitrosylation cascade involving SAR1 and SURF4, which act as S-nitrosylases, to inhibit PCSK9 secretion. These findings suggest a potential treatment paradigm for cardiovascular disease.
Article
Cardiac & Cardiovascular Systems
Konstantinos E. Hatzistergos, Adam R. Williams, Derek Dykxhoorn, Michael A. Bellio, Wendou Yu, Joshua M. Hare
CIRCULATION RESEARCH
(2019)
Article
Endocrinology & Metabolism
John Alden Lee, Manish Kuchakulla, Himanshu Arora, Shathiyah Kulandavelu, Evert Gonzalez, Thomas A. Masterson, Joshua M. Hare, Ursula B. Kaiser, Ranjith Ramasamy
FRONTIERS IN ENDOCRINOLOGY
(2019)
Meeting Abstract
Cell & Tissue Engineering
M. A. Bellio, M. Sharma, Y. Lee, S. Kulandavelu, M. Benny, K. Young, A. Khan
Meeting Abstract
Urology & Nephrology
J. Lee, M. Kuchakulla, H. Arora, S. Kulandavelu, T. Masterson, J. Hare, R. Ramasamy
JOURNAL OF SEXUAL MEDICINE
(2019)
Article
Cardiac & Cardiovascular Systems
Keyvan Yousefi, Camila I. Irion, Lauro M. Takeuchi, Wen Ding, Guerline Lambert, Trevor Eisenberg, Sarah Sukkar, Henk L. Granzier, Mei Methawasin, Dong Lee, Virginia S. Hahn, David A. Kass, Konstantinos E. Hatzistergos, Joshua M. Hare, Keith A. Webster, Lina A. Shehadeh
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2019)
Meeting Abstract
Urology & Nephrology
K. Chu, S. Kulandavelu, T. Masterson, E. Ibrahim, H. Arora, R. Ramasamy
JOURNAL OF SEXUAL MEDICINE
(2020)
Article
Multidisciplinary Sciences
Konstantinos E. Hatzistergos, Michael A. Durante, Krystalenia Valasaki, Amarylis C. B. A. Wanschel, J. William Harbour, Joshua M. Hare
Article
Cardiac & Cardiovascular Systems
Shathiyah Kulandavelu, Raul A. Dulce, Christopher Murray, Michael A. Bellio, Julia Fritsch, Rosemeire Kanashiro-Takeuchi, Himanshu Arora, Ellena Paulino, Daniel Soetkamp, Wayne Balkan, Jenny E. Van Eyk, Joshua M. Hare
Summary: The deficiency of GSNOR can lead to imbalanced placental S-nitrosylation and induce preeclampsia in mice, characterized by symptoms such as hypertension, proteinuria, and renal pathology. Ascorbate can reverse these pathological changes, except for fetal weight. The decreased GSNOR activity and increased nitrosative stress are also found in human preeclamptic placentas.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Cardiac & Cardiovascular Systems
Sana Nasim, Popular Pandey, Rosemeire M. Kanashiro-Takeuchi, Jin He, Joshua D. Hutcheson, Lidia Kos
Summary: The study demonstrates the importance of pigmentation in organizing elastic fibers and dictating biomechanical properties of the aortic valve in mice.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Raul A. Dulce, Rosemeire M. Kanashiro-Takeuchi, Lauro M. Takeuchi, Alessandro G. Salerno, Amarylis C. B. A. Wanschel, Shathiyah Kulandavelu, Wayne Balkan, Marilia S. S. R. Zuttion, Renzhi Cai, Andrew Schally, Joshua M. Hare
Summary: This study aimed to test the hypothesis that activating the GHRH receptor signalling pathway can prevent and reverse diastolic dysfunction and pathophysiologic features associated with HFpEF. The results showed that synthetic GHRH agonists can reduce myocardial fibrosis and hypertrophy, and improve cardiac performance.
CARDIOVASCULAR RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Camila I. Irion, Monique Williams, Jose Condor Capcha, Trevor Eisenberg, Guerline Lambert, Lauro M. Takeuchi, Grace Seo, Keyvan Yousefi, Rosemeire Kanashiro-Takeuchi, Keith A. Webster, Karen C. Young, Joshua M. Hare, Lina A. Shehadeh
Summary: Alport syndrome is a hereditary renal disorder with no known cure. This study explores the potential use of SGLT2 as a therapeutic target for Alport syndrome and finds that the response to treatment varies depending on the mouse strain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Eleftheria Maria Pechlivani, Sotirios Pemas, Alexandros Kanlis, Paraskevi Pechlivani, Spyros Petrakis, Athanasios Papadimitriou, Dimitrios Tzovaras, Konstantinos E. Hatzistergos
Summary: In the past decade, there has been significant progress in various types of bioreactors. However, the conventional bioreactors are often bulky and expensive, limiting accessibility. To address these challenges, this article introduces and evaluates a DIY 3D printed smart bioreactor, which offers a cost-effective and user-friendly solution for the proliferation of various bioentities.
Meeting Abstract
Urology & Nephrology
John Lee, Manish Kuchakulla, Himanshu Arora, Shathiyah Kulandavelu, Evert Gonzalez, Thomas Masterson, Joshua Hare, Ranjith Ramasamy
JOURNAL OF UROLOGY
(2019)
Meeting Abstract
Urology & Nephrology
Himanshu Arora, Kush Panara, Manish Kuchakulla, Shathiyah Kulandavelu, Kerry L. Burnstein, Andrew V. Schally, Joshua M. Hare, Ranjith Ramasamy
JOURNAL OF UROLOGY
(2019)
Article
Medicine, Research & Experimental
Michelle S. Parvatiyar, Alexandra J. Brownstein, Rosemeire M. Kanashiro-Takeuchi, Judd R. Collado, Karissa M. Dieseldorff Jones, Jay Gopal, Katherine G. Hammond, Jamie L. Marshall, Abel Ferrel, Aaron M. Beedle, Jeffrey S. Chamberlain, Jose Renato Pinto, Rachelle H. Crosbie