Journal
IRANIAN JOURNAL OF BIOTECHNOLOGY
Volume 13, Issue 1, Pages 36-42Publisher
NATL INST GENETIC ENGINEERING & BIOTECHNOLOGY
DOI: 10.15171/ijb.1034
Keywords
Adulterant identification; Classification; Crocus sativus; DNA barcoding
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Funding
- National Natural Science Foundation of China [31161140345]
- Ministry of Education of China [B08044, MUC 2015MDTD16C, YLDX01013]
- Ministry of Science and Technology of China [2012FY110300]
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Background: Saffron (Crocus sativus L.) is a common but very expensive herbal medicine. As an important traditional medicine, it has an outstanding effect in treating irregular and painful menstruation. Recently, the over-demand tendency of saffron results in an unusual phenomenon in the medicinal markets. Adulterants and saffron-like substitutes are intentionally mixed into medicinal markets and pharmacies or online stores, affecting drug safety and food quality. Objectives: Our study aimed to identify saffron from its adulterants via DNA barcoding. Materials and Methods: Samples (13 saffron + 4 others containing Carthamus tinctorius or Chrysanthemum x morifolium) obtained from 12 different provinces of China. Through DNA barcoding, samples were compared using three candidate markers, trnH-psbA, rbcL-a and ITS2. Results: trnH-psbA and rbcL-a were capable of distinguishing different accessions. ITS2 could identify samples even at intra-specific level. According to these three barcodes, four samples were identified saffron-like substitutes. Conclusions: The adulterant rate in Chinese markets reaches as high as 33.33% that may cause health risks and further may reduce saffron efficacy once is being used as herbal remedy. In order to make a distinction between C. sativus with other genera as adulterants, DNA barcoding is suggested.
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