Article
Polymer Science
Gerke H. Schuiringa, Marko Mihajlovic, Corrinus C. van Donkelaar, Tina Vermonden, Keita Ito
Summary: This study explores a new approach using methacrylated natural polymer hydrogel to mimic the load-bearing properties of cartilage. The results show that using this hydrogel construct can reduce the volumetric swelling of the hydrogel and achieve similar moduli and time constants compared to native bovine cartilage after 28 days.
Review
Biochemistry & Molecular Biology
Xiaoli Yang, Xiaojing Li, Zhaoping Wu, Lingling Cao
Summary: This review first introduces the characteristics of common natural polysaccharide- and protein-based hydrogels and the processes used for methacrylate group modification. Next, the applications of methacrylated natural hydrogels in tissue engineering are discussed. Finally, existing methacrylated natural hydrogels are summarized and discussed in terms of limitations and future developments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Seongwon Lee, Joohee Choi, Jina Youn, Younghun Lee, Wooyoup Kim, Seungho Choe, Jeongeun Song, Rui L. Reis, Gilson Khang
Summary: This study investigated a ternary hydrogel composed of gellan gum, silk fibroin, and chondroitin sulfate as a biomaterial for cartilage tissue engineering. The hydrogel showed enhanced mechanical properties with higher gellan gum content, but exhibited harsh conditions for cell encapsulation at certain concentrations. The ternary hydrogel exhibited positive cell growth and expression of cartilage-specific ECM and genes, indicating its potential for promoting articular cartilage defect repair in cartilage tissue engineering.
Article
Chemistry, Applied
Xin Liu, Shuai Liu, Rong Yang, Penghui Wang, Wenjie Zhang, Xiaoyan Tan, Yanhan Ren, Bo Chi
Summary: A gradient hydrogel scaffold was designed based on the structure and composition distribution in biological cartilage tissue, showing excellent physical properties and cell compatibility. The scaffold can mimic the function of natural cartilage, making it a potential option for cartilage tissue engineering.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Applied
Swati Sharma, Harishkumar Madhyastha, Shrikant Sitaram Kirwale, Kentaro Sakai, Yash T. Katakia, Syamantak Majumder, Aniruddha Roy
Summary: This study developed a unique wound scaffold material (CH-CS PEC) with excellent antibacterial and anti-inflammatory properties. The results showed that CH-CS PEC could promote wound healing in infected wounds by inducing bacterial cell death and modulating macrophage function.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Shrikant Sitaram Kirwale, Swati Sharma, Aniruddha Roy
Summary: Current cell grafting techniques primarily rely on seeding cells on pre-formed scaffolds, which do not mimic the tissue's 3D architecture. This study evaluated a strategy based on poly-electrolyte complexation for 3D engraftment of cells in a porous polymeric scaffold. The results demonstrated that the in-situ forming PEC of CH, CS, and HA had good physicochemical properties for cell grafting and supported grafted cells with improved function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Lin Huang, Wantao Wang, Yiwen Xian, Lei Liu, Jinghao Fan, Hongmei Liu, Zhaomin Zheng, Decheng Wu
Summary: This study reported a rapidly forming injectable chitosan/PEG hydrogel (CSMA-PEGDA-L) that can retard the progression of intervertebral disc (IVD) degeneration. The hydrogel demonstrated strong compressive strength and low cytotoxicity. When implanted on the punctured IVD of rats, it effectively slowed down the degenerative changes.
MATERIALS TODAY BIO
(2023)
Article
Polymer Science
Haonan Wang, Qian Li, Yongchao Jiang, Xiaofeng Wang
Summary: This study designed gelatin-based hydrogels loaded with chondroitin sulfate (CS) to regulate the angiogenesis behaviors of endothelial cells (ECs). The hydrogels exhibited excellent biocompatibility and hemocompatibility, and significantly promoted ECs angiogenesis behaviors. However, excessive loading of CS weakened the effect.
Article
Biochemistry & Molecular Biology
Jijo Thomas, Vianni Chopra, Swati Rajput, Rajdeep Guha, Naibedya Chattopadhyay, Deepa Ghosh
Summary: Injectable hydrogels have been used for cartilage repair, but some of them have issues with rapid degradation and low mechanical strength. To address these challenges, a bioinspired double network hydrogel (BDNH) was developed, which exhibits temperature-dependent stiffening after implantation. The BDNH showed excellent cell viability, long time cell proliferation, and cartilage specific matrix production, making it a potential candidate for cartilage tissue engineering.
Article
Polymer Science
Tien Thinh Nguyen, Le Hang Dang, Phuong Nguyen, Truc Le-Buu Pham, Hai Khoa Le, Minh-Ty Nguyen, Tran Thi Yen Nhi, Sijia Feng, Jun Chen, Ngoc Quyen Tran
Summary: Hydrogels with bone biomimetic microenvironment are prepared using biopolymer and biphasic calcium phosphate nanoparticles, which have great potential for bone-graft materials development.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Polymer Science
Yaqi Zhao, Yan Li, Tianchan Lan, Baowei Wang, Ming Huang, He Huang, Changming Qiao, Jingxin Sun
Summary: The physicochemical properties and microstructure of hybrid hydrogels prepared using sodium alginate and chondroitin sulfate extracted from two animal sources were investigated. The results showed that the addition of different sources of sulfate greatly influenced the properties and microstructure of the hydrogels.
Article
Materials Science, Characterization & Testing
N. Rashidi, M. Tamaddon, C. Liu, J. Czernuszka
Summary: This study aimed to improve the application of chondroitin sulfate (CS) in tissue engineering and drug delivery devices by polymerizing it into a higher molecular weight polymer (PCS). The physicochemical properties of PCS were characterized and compared with CS, showing significant improvements in diameter and viscoelastic properties. These results suggest a great potential for using PCS in regenerative medicine devices.
Article
Polymer Science
Rubina Qaiser, Fahad Pervaiz, Hanasul Hanan, Hina Shoukat, Muhammad Nadeem
Summary: In this research, a mucoadhesive hydrogel loaded with captopril was developed using chondroitin sulfate (CHS), polyvinylpyrrolidone (PVP), and 2-acrylamide-2-methylpropane sulphonic acid (AMPS). The hydrogel showed efficient sustained release of the drug and demonstrated enhanced mucoadhesive characteristics. The study also analyzed the influence of different formulation ingredients and quantities on the swelling behavior and mucoadhesion capacity of the hydrogel. The findings suggest that this CHS/PVP-co-poly (AMPS)-based hydrogel system is a viable option for sustained delivery of captopril.
Article
Biochemistry & Molecular Biology
Vadym Burchak, Fritz Koch, Leonard Siebler, Sonja Haase, Verena K. Horner, Xenia Kempter, G. Bjoern Stark, Ute Schepers, Alisa Grimm, Stefan Zimmermann, Peter Koltay, Sandra Strassburg, Gunter Finkenzeller, Filip Simunovic, Florian Lampert
Summary: This study evaluated the printability and biocompatibility of a semi-synthetic hydrogel system (GelNB/GelS) for bioprinting of adipose-tissue-derived mesenchymal stem cells (ASCs). The results showed that GelNB/GelS hydrogels supported ASCs viability, proliferation and differentiation. The mechanical properties of GelNB/GelS system influenced the differentiation fate of ASCs. The hydrogel system may have potential applications in in vivo tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
B. Velasco-Rodriguez, T. Diaz-Vidal, L. C. Rosales-Rivera, C. A. Garcia-Gonzalez, C. Alvarez-Lorenzo, A. Al-Modlej, V. Dominguez-Arca, G. Prieto, S. Barbosa, J. F. A. Soltero Martinez, P. Taboada
Summary: The combination of methacroyl-modified HA and Gel in hybrid hydrogels result in improved mechanical properties and a stronger structure. The presence of HAMA leads to stiffer, less porous hydrogels that are more resistant to collagenase degradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Biomaterials
Saeed Samani, Shahin Bonakdar, Ali Farzin, Jamshid Hadjati, Mahmoud Azami
Summary: A novel method for synthesizing photocrosslinkable chitosan hydrogel was introduced in this study. Methacrylated chitosan obtained under controlled conditions and using EDC/NHS showed significant coupling efficiency and gel formation capacity, with interconnected pores and swelling ratios, providing new aspects for developing novel biomaterials in cell-based therapies.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2021)
Article
Polymer Science
Behzad Bolandi, Rana Imani, Shahin Bonakdar, Hossein Fakhrzadeh
Summary: The study focuses on developing a composite injectable hydrogel that can support cartilage regeneration by capturing cells and growth factors. The fabricated hydrogel, incorporating platelet rich plasma encapsulated microbeads, shows sustained release of growth factors and promotes tissue regeneration.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Materials Science, Textiles
Mahsa Janmohammadi, Mohammad Sadegh Nourbakhsh, Shahin Bonakdar
Summary: Skin tissue engineering is evolving with the use of electrospun scaffolds based on polycaprolactone (PCL) and hyaluronic acid (HA) containing L-ascorbic acid (AA). The combination of PCL and HA in the scaffolds enhance hydrophilicity and degradation rate, while promoting cell attachment, viability, and proliferation. The nanofibrous scaffold could potentially be applied for skin tissue engineering with improved outcomes.
FIBERS AND POLYMERS
(2021)
Article
Polymer Science
Sadaf Dadashkhan, Shiva Irani, Shahin Bonakdar, Behafarid Ghalandari
Summary: In this study, adipose derived stem cells (ADSCs) were successfully differentiated into Schwann cells (SCs) using imprinting method and beta-carotene. The topography of SCs was imprinted on PDMS substrates, inducing differentiation signals in MSCs and upregulation of SC specific markers with the addition of beta-carotene. This study demonstrated the ability of Schwann cell imprinted substrates and beta-carotene to mimic SC morphology and induce neural differentiation in MSCs, without the need for expensive or unsafe chemical growth factors.
JOURNAL OF APPLIED POLYMER SCIENCE
(2021)
Article
Materials Science, Biomaterials
Sepideh Yazdian Kashani, Mostafa Keshavarz Moraveji, Mojtaba Taghipoor, Reza Kowsari-Esfahan, Ali Akbar Hosseini, Leila Montazeri, Mohammad Mehdi Dehghan, Hossein Gholami, Saeed Farzad-Mohajeri, Morteza Mehrjoo, Mohammad Majidi, Philippe Renaud, Shahin Bonakdar
Summary: This study demonstrates the potential of the imprinting method in inducing chondrogenicity in stem cells, with successful regeneration of articular cartilage defects in rabbits after transplantation. The efficiency of the imprinting method was increased using microfluidic chip design and fabrication, leading to the precise placement of stem cells on chondrocyte patterns on the substrate.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Materials Science, Biomaterials
Leila Montazeri, Reza Kowsari-Esfahan, Sara Pahlavan, Motahareh Sobat, Shahram Rabbani, Hassan Ansari, Fahimeh Varzideh, Maryam Barekat, Sarah Rajabi, Fatemeh Navaee, Shahin Bonakdar, Philippe Renaud, Thomas Braun, Hossein Baharvand
Summary: In this study, a novel approach of co-transplantation of cardiac progenitor cells and oxygen generating microparticles was proposed for cell therapy of acute myocardial infarction. The strategy significantly improved heart function and reduced fibrotic tissue formation, potentially by providing an oxygen-rich microenvironment that promotes cell retention and differentiation.
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Engineering, Biomedical
Maryam Khajavi, Abdolmajid Hajimoradloo, Mojgan Zandi, Mohamad Pezeshki-Modaress, Shahin Bonakdar, Abbas Zamani
Summary: The study successfully engineered a cartilage-like scaffold using ECM from sturgeon fish cartilage to stimulate cartilaginous matrix synthesis in human adipose stem cells. The porous scaffold derived from fish cartilage ECM showed excellent potential for cartilage tissue engineering, supporting cell adhesion, proliferation, and chondrogenic differentiation. The scaffold was demonstrated to facilitate the synthesis of specific cartilage proteins, such as collagen type II and aggrecan, making it a promising option for tissue regeneration.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Veterinary Sciences
Mohammad Majidi, Fatemeh Heidarnejad, Mohsen Naseri, Shahin Bonakdar, Maryam Salimi, Roya Yaraee
Summary: Marham-Mafasel can reduce joint inflammation, decrease IL-1 beta gene expression, and show significant therapeutic effects in animal experiments.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Veterinary Sciences
Maryam Salimi, Abolfazl Shirazi, Koushan Sineh Sepehr, Mohsen Norouzian, Vahid Ebrahimi, Maryam Mehravar, Mohammad Majidi, Mohammad M. Mehrazar
Summary: The expression of trophectoderm, pluripotency, and imprinting genes in the Rag1 knocked-out embryos produced by the CRISPR/Cas9 system is significantly lower compared to embryos obtained by natural fertilization, potentially reducing embryo quality and live birth rates.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Cell Biology
Behnam Sadeghi, Elham Roshandel, Ali Pirsalehi, Sepide Kazemi, Ghazaleh Sankanian, Mohammad Majidi, Maryam Salimi, Nasser Aghdami, Hoda Sadrosadat, Sarvenaz Samadi Kochaksaraei, Farshid Alaeddini, Olle Ringden, Abbas Hajifathali
Summary: The study showed that DSC therapy is safe and effective for patients with COVID-19-induced ARDS, improving oxygenation levels, decreasing inflammatory cytokine levels, and clearing pulmonary infiltrates.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Mohammad Majidi, Mohammad Salehi, Maryam Salimi, Shahrokh Paktinat, Niloofar Sefati, Samaneh Montazeri, Arsalan Jalili, Mohsen Norouzian
Summary: The study demonstrated the effectiveness of melatonin in in vitro maturation, fertilization, and early development of embryos. Low doses of melatonin were found to enhance blastocyst development and affect the expression of HMGB1, an early development marker in mouse embryos.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Engineering, Biomedical
Zahra Sadat Ghazali, Mahnaz Eskandari, Shahin Bonakdar, Philippe Renaud, Omid Mashinchian, Shahriar Shalileh, Fabien Bonini, Ilker Uckay, Olivier Preynat-Seauve, Thomas Braschler
Summary: Cell-imprinting technology is a novel method that directs stem cell fate by using substrates molded from target cells. The study demonstrated that cell-imprinted substrates can accelerate neural differentiation and induce morphological changes in stem cells, leading to upregulation of neural stem cell and early neuronal markers.
Article
Chemistry, Multidisciplinary
Khorshid Kamguyan, Saeed Zajforoushan Moghaddam, Abolfazl Nazbar, Seyyed Mohammad Amin Haramshahi, Shiva Taheri, Shahin Bonakdar, Esben Thormann
Summary: The study found that different cell imprints possess distinct nanotopographical features, which can direct stem cell differentiation into specific lineages.
NANOSCALE ADVANCES
(2021)
