Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 27, Issue 1, Pages 181-184Publisher
SPRINGER
DOI: 10.1007/s11274-010-0429-0
Keywords
Loop-mediated isothermal amplification (LAMP); P. aeruginosa; Rapid detection
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Funding
- Science Foundation of Ministry of Education of China [706046]
- National Natural Science Foundation of China [20436020]
- China Scholarship Council [2008615044]
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We developed and evaluated the specificity and sensitivity of a simple loop-mediated isothermal amplification (LAMP) method for rapid detection of P. aeruginosa strains. The optimal reaction condition was found to be 65A degrees C for 45 min, with the detection limit as 100 fg DNA/tube and 10 CFU/reaction. Application of LAMP assays were performed 426 clinical samples (including 252 P. aeruginosa and 174 non- P. aeruginosa isolates) using a rapid procedure and easy result confirmation. Sensitivity of LAMP and PCR assays was found to be 97.6% (246/252) and 90.5% (228/252), respectively; with a 100% specificity for both assays.
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