Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
Volume 5, Issue 1, Pages 26-35Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpddr.2014.09.005
Keywords
Leishmania; Genome; Next generation sequencing; Drug resistance; Aneuploidy; Single nucleotide polymorphisms; Gene amplification
Categories
Funding
- Canadian Institutes for Health Research grant
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The use of next generation sequencing has the power to expedite the identification of drug resistance determinants and biomarkers and was applied successfully to drug resistance studies in Leishmania. This allowed the identification of modulation in gene expression, gene dosage alterations, changes in chromosome copy numbers and single nucleotide polymorphisms that correlated with resistance in Leishmania strains derived from the laboratory and from the field. An impressive heterogeneity at the population level was also observed, individual clones within populations often differing in both genotypes and phenotypes, hence complicating the elucidation of resistance mechanisms. This review summarizes the most recent highlights that whole genome sequencing brought to our understanding of Leishmania drug resistance and likely new directions. (C) 2014 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.
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