4.5 Article

Removal of Benzo[a]pyrene by a fungus Aspergillus sp BAP14

Journal

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 25, Issue 8, Pages 1395-1401

Publisher

SPRINGER
DOI: 10.1007/s11274-009-0026-2

Keywords

Biodegradation; Benzo[a]pyrene; Aspergillus sp BAP14; Morphologic performances

Funding

  1. Fork Ying Tong Education Foundation [94002]
  2. National Science Foundation of China [40206015]
  3. Science & Technology Project of Guangdong Province, P. R. China [2006A36502001, 2007A032600 003]

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A fungal strain BAP14 isolated from marine sediments of coast in Xiamen city, was found to have the ability to degrade benzo[a]pyrene (BaP), and identified as Aspergillus sp. based on 18S rRNA gene sequence. Aspergillus sp. BAP14 was able to remove about 30 and 60% of BaP with initial concentration of 10 mg l(-1) in 3 and 12 days of incubation, respectively. Addition of saccharides and low molecular weight polycyclic aromatic hydrocarbons appeared to have effect on the degradation ability, in particularly the addition of lactose and naphthalene. Furthermore, we demonstrated that lipidic particles could be observed in the presence of benzo[a]pyrene based on the morphologic performance of Aspergillus sp. BAP14 through scanning electronic microscopy (SEM) and atomic force microscopy (AFM), respectively.

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