Article
Food Science & Technology
Yuan-qing Hu, Wan-ying Wang, Turmidzi Fath, Feng-xia Li, Lin-fang Fang, Zan-hu Zhou, Dan-feng Zhang
Summary: In this study, a PMA-mPCR assay was developed for accurately screening viable vibrios in aquatic foods. The assay exhibited 100% specificity and maintained its sensitivity after PMA treatment. The results showed that the assay could detect viable V. parahaemolyticus, V. alginolyticus, and V. cholerae as low as 1.0 x 102 CFU/mL after 10 hours of enrichment. The practical application of the assay using 453 actual samples also demonstrated its accuracy in screening target vibrios in seafood.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Ning Yuan, Haoyu Yang, Yunzhe Zhang, Hancong Xu, Xin Lu, Hui Xu, Wei Zhang
Summary: A rapid detection method for V. parahaemolyticus based on RF-SRCA technology has been established in this study. Real-time fluorescence curve can effectively determine the results within a short time, and the method exhibits excellent specificity and sensitivity. RF-SRCA has higher sensitivity and shorter detection time compared to traditional methods, and can eliminate the risk of cross-contamination.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Karan Gautam Kaval, Suneeta Chimalapati, Sara D. Siegel, Nalleli Garcia, Jananee Jaishankar, Ankur B. Dalia, Kim Orth
Summary: It has been proposed that bacteria use a process called transertion to synthesize and insert membrane proteins. This study provides evidence that Vibrio parahaemolyticus uses transertion to assemble its type III secretion system and inject virulence factors into host cells.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma
Summary: BNNPs have been proven to significantly improve the specificity and sensitivity of PCR and LAMP amplification reactions, expanding the application range of nucleic acid detection and increasing detection sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Microbiology
Abu Baker Siddique, M. Moniruzzaman, Sobur Ali, Md Nayem Dewan, Mohammad Rafiqul Islam, Md Shafiqul Islam, Mohammed Badrul Amin, Dinesh Mondal, Anowar Khasru Parvez, Zahid Hayat Mahmud
Summary: The study investigated the incidence and virulence potential of V. parahaemolyticus isolates in aquaculture farms in Bangladesh, revealing high antibiotic resistance and O8:KUT as the predominant serotype. Environmental factors such as temperature and salinity were significantly correlated with V. parahaemolyticus contamination. The genetic variation and relatedness among pathogenic isolates were determined using ERIC-PCR.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Environmental Sciences
Md. Ashrafudoulla, Kyung Won Na, Md. Iqbal Hossain, Md. Furkanur Rahaman Mizan, Shamsun Nahar, Sazzad Hossen Toushik, Pantu Kumar Roy, Si Hong Park, Sang -Do Ha
Summary: Infections of gastroenteritis in humans are mainly caused by consumption of shellfish contaminated with Vibrio parahaemolyticus, leading to health and economic losses. Investigation on the virulence factor production, antibiotic resistance profile, and biofilm-forming capacity of Vibrio parahaemolyticus isolates showed high resistance to antibiotics and virulence factors in isolates from shellfish in Korea. Monitoring of these factors may help in decreasing V. parahaemolyticus infections and ensuring seafood safety.
MARINE POLLUTION BULLETIN
(2021)
Article
Food Science & Technology
Jung Hyen Lee, Minjin Oh, Byoung Sik Kim
Summary: VPG01 phage is an eco-friendly alternative to antibiotics for controlling Vibrio parahaemolyticus in seafood. It inhibits the growth of V. parahaemolyticus and significantly reduces the pathogen load in aquatic crustaceans and seafood.
Article
Engineering, Environmental
Zaafrane Sami, Maatouk Kaouthar, Cherif Nadia, Ben Mansour Hedi
Summary: This study investigated the impact of sunlight and salinities on the survival of Vibrio parahaemolyticus strains. The results showed that sunlight exposure caused the strains to become non-culturable but viable. The tdh positive strains were more culturable than the non-virulent or trh positive strain. The survival time was longer at certain salinities and the non-pathogenic strain survived longer than the virulent ones. Resuscitation experiments did not revive the non-culturable bacteria. Microscope observations showed that the strains remained motile after losing their culturability.
WATER ENVIRONMENT RESEARCH
(2022)
Article
Microbiology
Lili Chen, Jikai Wang, Jiang Chen, Ronghua Zhang, Hexiang Zhang, Xiaojuan Qi, Yue He
Summary: This study analyzed the epidemiological characteristics of Vibrio parahaemolyticus outbreaks in Zhejiang Province and provided insights for targeted prevention and control of foodborne diseases. The results showed that from 2010 to 2022, a total of 383 outbreaks caused by Vibrio parahaemolyticus were reported, resulting in 4,382 illnesses, 326 hospitalizations, and 1 death. The majority of outbreaks occurred between July and September, and outbreaks associated with restaurants were the most common.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Eleonora Di Salvo, Felice Panebianco, Antonio Panebianco, Graziella Ziino
Summary: This study found that viable but nonculturable forms of Vibrio parahaemolyticus may be present in frozen bivalve molluscs, suggesting that it could be a potential unrecognized source of food contamination and infection. Further data is needed to perform a robust risk assessment.
Article
Food Science & Technology
Monica Campas, Sandra Leonardo, Ainoa Ferre-Gode, Asif A. Chowdhury, Anna Toldra, Karl B. Andree, Ana Roque
Summary: Vibrio parahaemolyticus is a dominant cause of foodborne infections and can accumulate in shellfish. A duplex electrochemical biosensor was developed to detect the tdh and trh genes of V. parahaemolyticus in oysters, providing a quick and specific method for monitoring this pathogen.
Article
Biotechnology & Applied Microbiology
Pham Thi Hai Yen, Nguyen Quang Linh, Nguyen Duy Quynh Tram
Summary: Data was collected from 30 strains of Vibrio sampled from different organs of Sciaenops ocellatus infection. Results showed high similarity in 16S rRNA region nucleotide sequences with published species, and the presence of toxin genes in most Vibrio strains. Six parameters were used to measure DNA polymorphism in this population, revealing high diversity. A phylogenetic tree divided the strains into six groups based on their genetic differences. The study also identified the presence of specific Vibrio species on different organs of the infected fish.
Article
Gastroenterology & Hepatology
Yiquan Zhang, Yue Qiu, Xingfan Xue, Miaomiao Zhang, Junfang Sun, Xue Li, Lingfei Hu, Zhe Yin, Wenhui Yang, Renfei Lu, Dongsheng Zhou
Summary: QsvR directly repressed the transcription of toxR and calR while activating the transcription of cpsQ-mfpABC and mfpABC. This study provides deeper insights into the regulatory network of the mfp locus in Vibrio parahaemolyticus.
Article
Food Science & Technology
Corinne Audemard, Tal Ben-Horin, Howard I. Kator, Kimberly S. Reece
Summary: This study found that concentrations of bacteria in seawater can predictably reflect concentrations of the same bacteria in oysters. In regions with low tidal range, seawater samples can be used as an alternative method to assess the risk associated with oysters.
Article
Microbiology
Zhuosheng Liu, Chao Liao, Luxin Wang
Summary: In this study, the population dynamics of Vibrio parahaemolyticus (Vp) strains with different virulence genes were characterized by inoculating them into seawater at temperatures mimicking different seasons. The transcriptomic responses of the strains containing the thermostable direct hemolysin (tdh+) gene and the tdh-related hemolysin (trh+) gene were investigated. The results showed that the tdh+ strain had a higher growth rate than the trh+ strain at 30 degrees C, while there was no difference between the two strains at 10 degrees C. The RNA sequencing results revealed specific metabolic changes in the trh+ strain at 30 degrees C and downregulation of histidine metabolism and biofilm formation pathways in both strains at 10 degrees C. The expression of virulence-associated genes did not differ significantly between the two temperatures.
MICROBIOLOGY SPECTRUM
(2023)
