Article
Biochemistry & Molecular Biology
Warin Rangubpit, Eukote Suwan, Danai Sangthong, Kannika Wongpanit, Roger W. Stich, Prapasiri Pongprayoon, Sathaporn Jittapalapong
Summary: In this study, molecular dynamics simulations were conducted to investigate the binding of GTX and GSH to RmGST. It was found that GSH binds tighter and sits rigidly inside the G-site, while GTX occupies both active sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xingang Li, Yuanting Pang, Yiwang Zhong, Zhandong Cai, Qibin Ma, Ke Wen, Hai Nian
Summary: In this study, a tau-like glutathione transferase family gene named GmGSTU23 was identified in soybean, which showed a concentration-time-specific expression pattern in response to salt stress. Transgenic lines with GmGSTU23 displayed enhanced salt tolerance, root length, and fresh weight compared to the wild type. Changes in glutathione pools and associated enzyme activity were observed, indicating that GmGSTU23 enhances the activity of glutathione transferase to scavenge reactive oxygen species and glutathione, thereby conferring enhanced tolerance to salt stress in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Yushi Jiang, Yuzhe Zhang, Ruijie Duan, Jiayi Fan, Peng Jiao, Hongji Sun, Shuyan Guan, Siyan Liu
Summary: ZmGST26 was found to be downregulated in maize under drought stress. Overexpression of ZmGST26 in Arabidopsis resulted in increased sensitivity to drought stress and ABA, as well as inhibited ABA-mediated stomatal closure. ZmGST26 overexpression decreased the resistance of Arabidopsis to drought stress and led to the accumulation of reactive oxygen species.
Article
Neurosciences
Bo Cheng, Xiaoliang Hong, Linfang Wang, Yuanyuan Cao, Dengli Qin, Han Zhou, Dianshuai Gao
Summary: Curzerene inhibits the progression of glioma by suppressing the expression of GSTA4 and matrix metalloproteinase 9, and inhibiting the activation of the mTOR pathway. It also induces apoptosis and reduces invasion and migration of glioma cells. In vivo studies have shown that Curzerene significantly inhibits tumor growth and prolongs survival time.
CNS NEUROSCIENCE & THERAPEUTICS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Tiffany M. Russell, Des R. Richardson
Summary: GSTs are versatile proteins that play important roles in detoxification and cellular nitric oxide metabolism. They have the ability to convert organic nitrates into nitric oxide, thereby regulating vasodilation. GSTs are also considered suitable targets for pharmaceutical development, particularly in the field of anti-cancer drugs.
Article
Urology & Nephrology
Wenjun Zhang, Zheming Li, Miao Liu, Yi Mu, Jun He, Pan Chen, Dongdong Liu, Kehang Chen, Bangwei Che, Shenghan Xu, Hongyan Zhang, Kaifa Tang
Summary: The study demonstrated that gene polymorphism and metabolic syndrome may be potential risk factors for faster progression of benign prostatic enlargement and LUTS in BPH patients.
WORLD JOURNAL OF UROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yang Zhang, Ruhong Ming, Madiha Khan, Yue Wang, Bachar Dahro, Wei Xiao, Chunlong Li, Ji-Hong Liu
Summary: Plant ethylene-responsive factor PtrERF9 is up-regulated under cold stress, positively affecting freezing tolerance by modulating ROS homeostasis and activating PtrGSTU17 and PtrACS1 genes. PtrERF9 acts downstream of ethylene signaling, forming a feedback regulation loop to reinforce the transcriptional regulation of its target genes.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Microbiology
Alvaro de Obeso Fernandez del Valle, Christian Quintus Scheckhuber, David Armando Chavaro-Perez, Erandi Ortega-Barragan, Sutherland K. Maciver
Summary: Some members of Acanthamoeba are facultative pathogens that can infect the cornea, causing Acanthamoeba keratitis. During encystation, there is an upregulation of glutathione S-transferase (GST) genes, which play a role in maintaining redox balance. Inhibiting GST with ethacrynic acid decreases cell viability by 70%. Targeting GST and associated processes could be a potential treatment strategy to reduce relapses of Acanthamoeba infection.
Article
Chemistry, Analytical
Nan Wang, Lijun Zhang, Zhengxuan Li, Chenyu Zhou, Yuntai Lv, Xingguang Su
Summary: A stimulus-responsive hydrogel-based kit was constructed by synthesizing oxidized Pi@Ce-doped Zr-based MOFs and embedding them into a PVA hydrogel system, allowing real-time monitoring of GST using a smartphone. The color reaction of the kit was triggered by oxidized Pi@Ce-doped Zr-based MOFs and TMB, while the presence of GSH hindered the color reaction. However, catalyzed by GST, GSH could react with CDNB to initiate the color response of the kit. Combined with ImageJ software, the smartphone-acquired kit image information could be converted into hue intensity, providing a direct quantitative tool for GST detection with a detection limit of 0.19mU center dot L-1.
Article
Chemistry, Multidisciplinary
Miae Won, Seyoung Koo, Hao Li, Jonathan L. Sessler, Jin Yong Lee, Amit Sharma, Jong Seung Kim
Summary: This study introduces a new construct, EA-BPS, which induces a synergistic antitumor effect through a combination of ROS production and GST-π inhibition, leading to enhanced cell-killing effect under hypoxic conditions. By making better use of available oxygen in tumor environments, improved therapeutic PDT outcomes can be achieved even in low oxygen conditions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Plant Sciences
Tiantian Liu, Jin Wang, Lin Chen, Shengxuan Liu, Tengfei Liu, Liu Yu, Jingjing Guo, Ye Chen, Yiling Zhang, Botao Song
Summary: Potato production is hindered by cold temperatures, but the plant hormone ABA can improve freezing tolerance. However, the ABA-mediated signaling pathway for freezing tolerance is not well understood. In this study, cold acclimation of potato genotypes was enhanced with increased ABA levels. Exogenous application of ABA or its inhibitor NDGA resulted in enhanced or reduced freezing tolerance, respectively. The transcription factor ScAREB4 was found to be upregulated after cold and ABA treatments, and its overexpression promoted freezing tolerance by increasing trehalose content and antioxidant capacity. The results suggest that increased ABA content is related to freezing tolerance in potato, and ScAREB4 functions as a downstream transcription factor in the ABA signaling pathway.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Food Science & Technology
Yu Zhang, Ke-Xin Cao, Qin-Jian Niu, Jiang Deng, Ling Zhao, Mahmoud Mohamed Khalil, Niel Alexander Karrow, Kamil Kuca, Lv-Hui Sun
Summary: This study aimed to identify the key glutathione S-transferase (GST) isozymes involved in the detoxification of Aflatoxin B1 (AFB1) in ducks' primary hepatocytes. The researchers isolated and cloned the full-length cDNA encoding 10 GST isozymes from ducks' liver. The results showed that overexpression of GST and GST3 mitigated the toxic effects of AFB1 and increased the detoxified product of AFB1 in ducks' primary hepatocytes.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Qingfu Zhu, Liu Huang, Qinsi Yang, Zheng Ao, Rui Yang, Jonathan Krzesniak, Doudou Lou, Liang Hu, Xiaodan Dai, Feng Guo, Fei Liu
Summary: Metabolomic analysis of plasma exosomes in patients with ESCC revealed 196 metabolites, with a marker set of 3'-UMP, palmitoleic acid, palmitaldehyde, and isobutyl decanoate showing high accuracy in predicting ESCC recurrence. These exosome metabolome signatures, likely associated with cancer metabolism, could potentially serve as novel biomarkers for diagnosis and prognosis of ESCC.
Article
Plant Sciences
Aiqin Zhang, Rui Luo, Jiawen Li, Rongqing Miao, Hui An, Xiufeng Yan, Qiuying Pang
Summary: This study provides direct evidence that GSTU20 and GSTF11 are critically involved in aliphatic GSL biosynthesis, revealing their non-redundant but partially overlapping roles in GSL metabolism, with GSTU20 playing a more important role than GSTF11.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Lulu Han, Lin Zhou, Hongzhu Zou, Meng Yuan, Yan Wang
Summary: Anthocyanins, the important chromogenic substances responsible for the coloration of flowers, are transported and accumulated predominantly by PsGSTF3 gene. Silencing of PsGSTF3 gene leads to decreased anthocyanin accumulation and affects structural genes in the anthocyanin biosynthesis pathway. Heterologous overexpression of PsGSTF3 enhances anthocyanin accumulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)