Article
Medicine, Research & Experimental
Guang-Peng Zhou, Shi-Peng Li, Yi-Zhou Jiang, Jie Sun, Yu-Le Tan, Zhi-Gui Zeng, Lin Wei, Wei Qu, Li-Ying Sun, Zhi-Jun Zhu
Summary: This study demonstrates that using explanted livers from patients with inherited metabolic liver diseases as a cell source for hepatocyte transplantation can effectively treat acute liver failure. This therapeutic strategy significantly reduces mortality, improves liver function, decreases inflammatory cytokine levels, reduces hepatocyte apoptosis, and successfully localizes human hepatocytes in injured mouse livers.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Camille Le Guilcher, Gregory Merlen, Alessandra Dellaquila, Marie-Noeurolle Labour, Rachida Aid, Thierry Tordjmann, Didier Letourneur, Teresa Simon-Yarza
Summary: Porous pullulan-dextran hydrogel can be used to self-assemble hepatocytes and biliary-like cells into functional 3D microtissues, showing potential value in clinical applications.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Yinpeng Jin, Rongfeng Shi, Tangkai Qi, Qiang Li, Cong Chen, Shane Gao, Fengjuan Gao, Danjing Yang, Guangxi Sun, Jianqing Xu, Qingchun Fu, Jun Xu, Xiaoyan Zhang
Summary: Hepatocyte transplantation is hindered by limited resources, but mesenchymal stem cells (MSCs) can differentiate into hepatocyte-like cells (HLCs) and provide some hepatocyte functions. In this study, human adipose-derived stem cells (hADSCs) were found to have stronger hepatic differentiation ability compared to umbilical cord MSCs (hUCMSCs). The use of hADSCs-HLC or a combination of hADSCs and hADSCs-HLC had a better curative effect in treating acute liver failure, leading to hepatocyte regeneration, liver function recovery, reduced systemic inflammatory reaction, and improved survival rate.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Review
Cell Biology
Sunitha Vimalesvaran, Jessica Nulty, Anil Dhawan
Summary: Liver transplantation is the most effective treatment for pediatric end-stage liver disease, but its wider application is hindered by various limitations. Cellular therapies, such as hepatocytes and mesenchymal stromal cells (MSCs), are emerging as attractive alternatives. This review presents the world experience in hepatocyte and MSC transplantation and explores their potential for future effective applications.
Article
Engineering, Biomedical
Gyunggyu Lee, Hyemin Kim, Ji Young Park, Gyeongmin Kim, Jiyou Han, Seok Chung, Ji Hun Yang, Jang Su Jeon, Dong-Hun Woo, Choongseong Han, Sang Kyum Kim, Han-Jin Park, Jong-Hoon Kim
Summary: Advances in pluripotent stem cell technology have led to the efficient and reproducible generation of hepatocyte-like cell spheroids in various sizes using microwell culture platforms. These spheroids show high expressions of hepatic marker genes, enhanced drug-metabolizing activity, and increased sensitivity to hepatotoxicants compared to traditional hepatocytes. This imaging-based toxicity screening system holds promise for predicting drug-induced hepatotoxicity in the early stages of drug discovery.
Article
Cell & Tissue Engineering
Ka Zhang, Haixia Sun, Huijuan Cao, Yifan Jia, Xin Shu, Hong Cao, Yufeng Zhang, Xiaoan Yang
Summary: The therapeutic effects of umbilical cord mesenchymal stem cells (UCMSCs) for HBV-related acute-on-chronic liver failure and liver cirrhosis varied partly by patient age. Older patients showed greater decreases in TBIL and ALT levels after treatment. During UCMSC treatment, ACLF patients had significantly greater decreases in ALT levels compared to LC patients.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Medicine, General & Internal
Arshi Khanam, Shyam Kottilil
Summary: ACLF is a complex disease that can occur in patients with or without liver cirrhosis, with pathophysiological mechanisms involving systemic inflammation and acute hepatic insult. Currently, no specific and definitive treatment is available apart from liver transplantation. Regenerative medicine using MSCs shows potential as an alternative treatment for ACLF.
FRONTIERS IN MEDICINE
(2021)
Article
Gastroenterology & Hepatology
Cornelius Engelmann, Adam Herber, Annegret Franke, Tony Bruns, Philipp Reuken, Ingolf Schiefke, Alexander Zipprich, Stefan Zeuzem, Tobias Goeser, Ali Canbay, Christoph Berg, Jonel Trebicka, Frank E. Uschner, Johannes Chang, Tobias Mueller, Niklas Aehling, Moritz Schmelzle, Katrin Splith, Frank Lammert, Christian M. Lange, Christoph Sarrazin, Christian Trautwein, Michael Manns, Dieter Haeussinger, Jan Pfeiffenberger, Peter R. Galle, Anett Schmiedeknecht, Thomas Berg
Summary: In this multicenter controlled trial, G-CSF did not show significant beneficial effects on ACLF patients compared to standard medical therapy alone. The study was terminated prematurely due to futility after analysis. Therefore, G-CSF should not be recommended as a standard treatment for ACLF.
JOURNAL OF HEPATOLOGY
(2021)
Review
Gastroenterology & Hepatology
Jingfeng Liu, Zhiming Yuan, Qingwen Wang
Summary: Liver disease is a significant burden worldwide, and liver transplantation is the only option for end-stage or acute organ failure. However, the shortage of suitable donors and the need for aggressive immunosuppressive drugs limit its wide usage. Pluripotent stem cells derived hepatocytes provide a promising alternative with unlimited proliferative and differential potential. This review discusses the recent progress and future directions of using pluripotent stem cell-derived hepatocytes in treating acute liver failure and explores the opportunities and challenges in their clinical applications.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Yang Li, Xia Yang, Richie Plummer, Yoshihito Hayashi, Xiao-Shan Deng, Yun-Zhong Nie, Hideki Taniguchi
Summary: Liver disease is a global health issue, and organ transplantation is the only effective therapy but is hindered by donor shortage. Human pluripotent stem cells (hPSCs) are used for studying liver biology and pathology, developing alternative therapies. hPSCs can differentiate into multiple cell types for generating models to investigate and replicate biological activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Gastroenterology & Hepatology
Florent Artru, David Goldberg, Patrick S. Kamath
Summary: The authors debate whether patients with acute-on-chronic liver failure grade 3 (ACLF-3) should have higher liver transplant priority. They present a clinical case of a 62-year-old male with decompensated alcohol-associated cirrhosis and multiple comorbidities. The patient is at an exceedingly high risk of death without liver transplantation based on the severity of his liver disease and multiorgan failure.
JOURNAL OF HEPATOLOGY
(2023)
Review
Gastroenterology & Hepatology
Maria Pilar Ballester, Ahmed Elshabrawi, Rajiv Jalan
Summary: Acute-on-chronic liver failure (ACLF) is a life-threatening condition characterized by acute decompensation, organ failure, and high mortality risk. Extracorporeal liver support systems have been developed to improve outcomes, but their impact on survival remains unclear. DIALIVE, a novel liver support device, shows promise in reducing ACLF progression and is associated with faster recovery compared to standard treatment. Liver transplantation is a lifesaving option for severe ACLF patients, but careful patient selection is essential for optimal outcomes. This review discusses the current perspectives on extracorporeal liver support and liver transplantation for ACLF patients.
LIVER INTERNATIONAL
(2023)
Editorial Material
Hematology
Benny J. Chen
Summary: In this study, a novel mouse model of central nervous system chronic graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (HSCT) is presented, and unique mediators of this understudied complication are identified.
Review
Gastroenterology & Hepatology
William Bernal, Constantine Karvellas, Faouzi Saliba, Fuat H. Saner, Philippe Meersseman
Summary: Acute-on-chronic liver failure syndrome involves liver function deterioration, extrahepatic organ failure, and high mortality rate, requiring rapid and coherent management in the intensive care unit to improve patient outcomes.
JOURNAL OF HEPATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pallavi Subramanian, Jochen Hampe, Frank Tacke, Triantafyllos Chavakis
Summary: The prevalence of nonalcoholic fatty liver disease (NAFLD), also known as metabolic dysfunction associated fatty liver disease (MAFLD), is increasing globally. This disease spectrum includes benign hepatic steatosis and more advanced non-alcoholic steatohepatitis (NASH), which carries a higher risk of liver fibrosis, cirrhosis, and hepatocellular carcinoma. The activation of hepatic stellate cells (HSC) plays a key role in NASH-related fibrosis, and the intercellular interaction between hepatocytes and macrophages with HSC is a crucial factor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Salvatore Gruttadauria, Floriana Barbera, Pier Giulio Conaldi, Duilio Pagano, Rosa Liotta, Enrico Gringeri, Roberto Miraglia, Gaetano Burgio, Marco Barbara, Giada Pietrosi, Calogero Camma, Fabrizio Di Francesco
Summary: Hepatocellular carcinoma (HCC) recurrence is a major issue post liver resection, with clinical factors and loss of heterozygosity in the PTEN locus playing a role in predicting and potentially reducing the risk of recurrence. Multiple cancer gene alterations are associated with HCC progression, with specific AI mutations presenting a protective effect on recurrence risk.
Letter
Surgery
Salvatore Gruttadauria, Marco Barbara, Rosa Liotta
UPDATES IN SURGERY
(2021)
Article
Surgery
Renato Romagnoli, Salvatore Gruttadauria, Giuseppe Tisone, Giuseppe Maria Ettorre, Luciano De Carlis, Silvia Martini, Francesco Tandoi, Silvia Trapani, Margherita Saracco, Angelo Luca, Tommaso Maria Manzia, Ubaldo Visco Comandini, Riccardo De Carlis, Valeria Ghisetti, Rossana Cavallo, Massimo Cardillo, Paolo Antonio Grossi
Summary: In specific circumstances, liver transplant from donors with active COVID-19 may be considered for recipients with SARS-CoV-2 immunity to safely expand the donor pool and minimize transmission risks.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Surgery
Aurelio Seidita, Rosalia Longo, Fabrizio Di Francesco, Alessandro Tropea, Sergio Calamia, Giovanna Panarello, Marco Barbara, Salvatore Gruttadauria
Summary: The use of normothermic machine perfusion (NMP) to test liver viability before transplantation allows for the preventive use of very marginal grafts, increasing the number of transplants in low donation areas without negatively influencing patient outcomes.
UPDATES IN SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Mariangela Pampalone, Giampiero Vitale, Salvatore Gruttadauria, Giandomenico Amico, Gioacchin Iannolo, Bruno Douradinha, Alessandra Mularoni, Pier Giulio Conaldi, Giada Pietrosi
Summary: This study demonstrates the potential usefulness of hA-MSCs in treating carbapenem-resistant bacterial infections in ascites, with significant effects on bacterial load and immune factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Surgery
Alessandro Tropea, Marco Barbara, Duilio Pagano, Gianluca Marrone, Ioannis Petridis, Sergio Li Petri, Davide Cintorino, Salvatore Gruttadauria
Summary: This case report describes a 32-year-old female patient with biliary atresia who underwent three liver transplant surgeries. Intraoperative exploration revealed the presence of multiple collaterals and large splenocaval shunts, causing significant damage to the portal flow, which was successfully restored through surgical intervention.
Article
Oncology
Salvatore Gruttadauria, Floriana Barbera, Duilio Pagano, Rosa Liotta, Roberto Miraglia, Marco Barbara, Maria Grazia Bavetta, Calogero Camma, Ioannis Petridis, Daniele Di Carlo, Pier Giulio Conaldi, Fabrizio Di Francesco
Summary: Intrahepatic cholangiocarcinoma (iCCA) is a rare and aggressive liver tumor, with increasing incidence and mortality rates even after radical resection. Recent attention has focused on the disease, supporting liver transplantation as a viable treatment option. Genetic mutations activating signaling pathways have been linked to iCCA tumorigenesis, with a strong association found between Notch pathway mutations and tumor size. Efforts in molecular analysis may improve diagnostic capabilities and treatment protocols for better long-term survival in iCCA and HCC-iCCA patients.
Letter
Surgery
Salvatore Gruttadauria, Fabrizio Di Francesco, Roberto Miraglia
UPDATES IN SURGERY
(2022)
Article
Immunology
Alessandra Agnese Grossi, Federico Nicoli, Massimo Cardillo, Salvatore Gruttadauria, Giuseppe Tisone, Giuseppe Maria Ettorre, Luciano De Carlis, Renato Romagnoli, Carlo Petrini, Paolo Antonio Grossi, Mario Picozzi
Summary: The ethical analysis of performing liver transplantation in patients with resolved or active COVID-19 shows that the decision is ethically justifiable. By considering medical indications, patient preferences, quality of life, and contextual features, the decision is made on a case-by-case basis. Shared decision-making allows for the integration of clinical options with the patient's subjective preferences and considerations, providing valid informed consent tailored to individual circumstances. Including carefully selected SARS-CoV-2 positive donors offers the opportunity to save lives for patients who may have limited chances of receiving a transplant.
TRANSPLANT INFECTIOUS DISEASE
(2022)
Article
Medicine, General & Internal
Gennaro Martucci, Matteo Rossetti, Sergio Li Petri, Rossella Alduino, Riccardo Volpes, Giovanna Panarello, Salvatore Gruttadauria, Gaetano Burgio, Antonio Arcadipane
Summary: This study retrospectively observed and analyzed the early use of continuous renal replacement therapy (CRRT) following orthotopic liver transplantation (OLT). The study found that the early use of CRRT had a low incidence and was associated with worse outcomes, including lower survival and higher postoperative creatinine levels. Factors such as preoperative severity and intraoperative hypotension episodes were found to be associated with the increased use of CRRT.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Surgery
Sergio Calamia, Marco Barbara, Calogero Cipolla, Nello Grassi, Gianni Pantuso, Sergio Li Petri, Duilio Pagano, Salvatore Gruttadauria
Summary: The study aimed to identify risk factors for biliary leakage (BL) after liver surgery. The results showed that BL occurrence was associated with bilio-digestive anastomosis, neoadjuvant chemotherapy, body mass index, anatomical resection, and blood loss. Identifying high-risk patients can guide the positioning of drainage after surgery.
UPDATES IN SURGERY
(2022)
Article
Gastroenterology & Hepatology
Felice Giuliante, Francesca Ratti, Elena Panettieri, Vincenzo Mazzaferro, Alfredo Guglielmi, Giuseppe M. Ettorre, Salvatore Gruttadauria, Fabrizio Di Benedetto, Umberto Cillo, Luciano De Carlis, Raffaele Dalla Valle, Alessandro Ferrero, Roberto Santambrogio, Francesco Ardito, Luca Aldrighetti
Summary: The use of minimally invasive liver surgery has greatly reduced surgical risk for hepatocellular carcinoma. This study analyzed the outcomes of minimally invasive liver surgery for small hepatocellular carcinoma tumors less than 3 cm in size.
Letter
Surgery
Salvatore Gruttadauria, Duilio Pagano, Fabrizio di Francesco
UPDATES IN SURGERY
(2023)
Article
Medicine, General & Internal
Duilio Pagano, Ester Badami, Giovanni Zito, Pier Giulio Conaldi, Ivan Vella, Barbara Buscemi, Giandomenico Amico, Rosalia Busa, Paola Salis, Sergio Li Petri, Fabrizio di Francesco, Sergio Calamia, Pasquale Bonsignore, Alessandro Tropea, Caterina Accardo, Salvatore Piazza, Salvatore Gruttadauria
Summary: This study aimed to evaluate the impact of the immune system after graft reperfusion during kidney transplantation in adults in order to identify predictive biomarkers. The study found that the percentage of T-lymphocytes in the liver perfusate was significantly associated with delayed functional recovery and secondary infection. However, there was no significant association between the proportions of NK cells and NK-T cells with clinical outcomes.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Clinical Neurology
Federica Avorio, Gianvincenzo Sparacia, Giovanna Russelli, Aurelio Seidita, Giuseppe Mamone, Rossella Alduino, Fabio Tuzzolino, Salvatore Gruttadauria, Roberto Miraglia, Matteo Bulati, Vincenzina Lo Re
Summary: This study aimed to determine whether cerebral small vessels disease (cSVD) predicts acute neurological complications (NCs) in liver transplant patients aged 65 years or older. The results showed that more severe cSVD was associated with seizures, longer hospital stay, and disability. Hepatic encephalopathy and ascites were predictors of NCs after liver transplantation.
NEUROLOGY INTERNATIONAL
(2022)
