Journal
WORLD JOURNAL OF GASTROENTEROLOGY
Volume 15, Issue 48, Pages 6117-6122Publisher
BAISHIDENG PUBLISHING GROUP INC
DOI: 10.3748/wjg.15.6117
Keywords
Osteoglycin; Transfection; Hepatocellular carcinoma; Neoplasm metastasis; Genes; Gelatinases
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Funding
- National Natural Science Foundation of China [30500586]
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AIM: To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells. METHODS: A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis. RESULTS: High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo. CONCLUSION: High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression. (C) 2009 The WJG Press and Baishideng. All rights reserved
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