Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Maolong Hu, Hongkun Zhang, Lingna Kong, Juanjuan Ma, Ting Wang, Xinyu Lu, Yue Guo, Jiefu Zhang, Rongzhan Guan, Pu Chu
Summary: The application of herbicides is an effective strategy for weed control, and the development of herbicide-resistant crops can enhance weed management. However, the use of the herbicide tribenuron-methyl (TBM) is limited in rapeseed fields due to rapeseed's sensitivity to TBM. A study was conducted on the TBM-resistant rapeseed mutant M342 and its wild-type plants, revealing that M342 exhibited improved tolerance to TBM and had higher levels of proteins related to non-target-site resistance (NTSR) to herbicides compared to the wild-type plants. Differentially accumulated proteins (DAPs) between the two genotypes were enriched in glutathione metabolism and oxidoreduction coenzyme metabolic process, providing protection against oxidative stress caused by TBM. These findings offer insights into the mechanism of NTSR in plants and contribute to the development of herbicide-resistant crops.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Biochemistry & Molecular Biology
Caiyue Liu, Tianbao Zhang, Xinsen Yang, Liu Wang, Yan Long, Agula Hasi, Xinwu Pei
Summary: In this study, a mutant with resistance to ALS-inhibiting herbicides was obtained from the elite cultivar Longya10 through EMS mutagenesis. The mutant showed significantly increased resistance to TBM and slight resistance to imazethapyr compared to Longya10. Biochemical analysis revealed a Pro197Ser mutation in the LuALS1 gene, which conferred high resistance to sulfonylurea herbicides in the mutant. Two CAPS markers were developed based on this mutation site for marker assistant selection in breeding. The mutant did not cause yield losses in natural field conditions, making it a valuable germplasm resource for breeding herbicide-resistant flax varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Food Science & Technology
So Hee Yang, Euyeon Kim, Hyosun Park, Yeonjong Koo
Summary: Herbicide resistance is an important trait in improving farming methods and crop productivity. This study demonstrates the usefulness of using CRISPR-Cas9 system to edit herbicide binding protein genes in tomatoes and confirms the feasibility of targeting site editing through sgRNA efficiency testing. The success of breeding herbicide-resistant transgenic tomatoes highlights the value of CRISPR-Cas9 as a tool for crop improvement.
APPLIED BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Ryan Widianto, Denny Kurniadie, Dedi Widayat, Uum Umiyati, Ceppy Nasahi, Santika Sari, Abdul Shukor Juraimi, Hisashi Kato-Noguchi
Summary: Monochoria vaginalis is an aquatic herbaceous plant that is often found as a noxious weed in paddy fields. This research evaluated the resistance levels of M. vaginalis to different groups of herbicides and investigated the mutations in the ALS gene of the resistant biotype. The study found that the resistant biotype had varying levels of resistance to different herbicides and had specific amino acid substitutions in the ALS protein. Further research is needed to understand the molecular basis of resistance mechanisms and to develop control strategies.
Article
Plant Sciences
Sarah Shah, Thierry Lonhienne, Cody-Ellen Murray, Yibi Chen, Katherine E. Dougan, Yu Shang Low, Craig M. Williams, Gerhard Schenk, Gimme H. Walter, Luke W. Guddat, Cheong Xin Chan
Summary: This study identified 12 common herbicide-target genes and their proteins from seven species of Weeds of National Significance in Australia using high-throughput genome sequencing. The study found high sequence conservation at the herbicide-target sites among the diverse weed species. Mutations observed in the proteins of fireweed and parthenium suggest resistance of these weeds to certain herbicides. These findings provide important insights for herbicide discovery and development.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Nan Wang, Shuang Bai, Feng Bei, Ning Zhao, Sisi Jia, Tao Jin, Jinxin Wang, Hengzhi Wang, Weitang Liu
Summary: This study reports on a population of Myosoton aquaticum L. that exhibits resistance to herbicides in the absence of known acetolactate synthase (ALS) resistance mutations, highlighting the role of non-target-site resistance mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Plant Sciences
Vanessa Vital Silva, Rafael Mendes, Andreia Suzukawa, Fernando Adegas, Francismar Marcelino-Guimaraes, Rubem Oliveira
Summary: The study confirmed cross-resistance of a weed population in Brazil to ALS inhibitors and identified a mutation in the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in Erigeron sumatrensis for resistance to ALS-inhibiting herbicides.
Article
Biotechnology & Applied Microbiology
Wegi A. Wuddineh, Xia Xu, Gan-Yuan Zhong
Summary: This study identified VvALS1 as the best candidate gene for providing resistance to ALS-inhibiting herbicides in grapevine. The researchers found that chlorsulfuron and imazapyr herbicides showed a linear response curve in grapevine callus and in vitro shoots. By introducing amino acid substitutions in VvALS gene, the transgenic plants exhibited high resistance to chlorsulfuron or imazapyr herbicides. This research highlights the potential use of VvALS mutations as a selectable marker and enables gene-editing in grapevine improvement.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Ingvild Loubet, Lucie Meyer, Severine Michel, Fanny Pernin, Sebastien Carrere, Benoit Barres, Valerie Le Corre, Christophe Delye
Summary: This study explores the diversity of non-target site resistance (NTSR) mechanisms in common ragweed populations from different regions in France. Transcriptome analysis identifies a wide range of genes associated with NTSR and reveals population-specific evolution in response to herbicide selective pressure. The findings contribute to a better understanding of the complexity and diversity of NTSR mechanisms.
Article
Environmental Sciences
Ning-Hai Lu, Meng-Yuan Zhang, Kun Jiang, Gui-Lei Hu, Bai-Zhong Zhang
Summary: Descurainia Sophia (L.) is a common weed that causes serious damage in wheat fields worldwide. It is primarily controlled by chemical herbicides, but the unscientific application of herbicides has led to the development of resistance in different populations of D. sophia. This study determined the mutational sites of the ALS gene in four populations and found that point mutations and down-regulation of ALS mRNA expression may play a role in the resistance of D. sophia to ALS inhibitor herbicides. These findings are valuable for managing ALS inhibitor herbicide-resistant D. sophia in wheat fields in China.
FRESENIUS ENVIRONMENTAL BULLETIN
(2022)
Article
Plant Sciences
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Maria D. Osuna, Blanca Garcia-Garcia, Joel Torra, Joao Portugal, Rafael De Prado
Summary: A study in southern Spain found that Sinapis alba populations have developed resistance to the acetolactate synthase-inhibiting herbicide tribenuron-methyl. The resistant populations showed significantly higher resistance to tribenuron-methyl compared to the susceptible population. A mutation in the ALS gene and P450-mediated metabolism were identified as contributing factors to the resistance. Alternative chemical control methods were effective in controlling both the resistant and susceptible populations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Eduardo Jose Haverroth, Fabricio Andre Musa, Filipe Kalikoski Coelho, Vanessa de Freitas Duarte, Mariana Barbosa Pereira, Marcelo Teixeira Pacheco, Luiz Carlos Federizzi, Carla Andrea Delatorre
Summary: This study aimed to determine the principal grain-filling component across the oat panicle and the effect of nitrogen (N) fertilizer dosage. Results indicate that the grain weight differences across the oat panicle and genotypes are mainly explained by grain-filling rate, and there are differences in grain-filling onset with no change in the effective grain-filling duration.
Article
Agronomy
Marlon O. Bastiani, Nilda Roma-Burgos, Ana C. Langaro, Reiofeli A. Salas-Perez, Christopher E. Rouse, Marcus V. Fipke, Fabiane P. Lamego
Summary: South African lovegrass is the most important weed in native pastures of southern Brazil, and glyphosate is the main control method. Potassium salt of glyphosate has the fastest effect on South African lovegrass, while younger plants are easier to control. Adding AMS can enhance the effect of glyphosate on drought-stressed lovegrass.
Article
Agronomy
Leonard Bonilha Piveta, Nilda Roma-Burgos, Jose Alberto Noldin, Vivian Ebeling Viana, Claudia de Oliveira, Fabiane Pinto Lamego, Luis Antonio de Avila
Summary: Rice, the staple food for many people worldwide, is affected by climatic changes impacting its yield and quality. Weedy rice, with high genetic diversity, is crucial for rice crop improvement. This study analyzed the physiological and molecular profiles of rice cultivars and weedy rice under heat and drought stress, revealing genotype-specific responses to stress conditions. Research suggests that certain genotypes perform well under stress conditions and can be used as a source of genetic diversity for rice breeding.
Article
Agronomy
Leonard Bonilla Piveta, Jose Alberto Noldin, Nilda Roma-Burgos, Vivian Ebeling Viana, Lariza Benedetti, Jesus Juares Oliveira Pinto, Fabiane Pinto Lamego, Luis Antonio de Avila
Summary: This study characterized the morphological diversity of weedy rice in southern Brazil, analyzing 249 accessions from Rio Grande do Sul (RS) and Santa Catarina (SC) states. All accessions were of indica lineage and separated into 14 distinct groups based on phenotypic traits, with high variability in flowering time, indicating potential for gene flow and diverse responses to weed management in herbicide-resistant rice planting.
Article
Agronomy
Carla Andrea Delatorre, Vanessa de Freitas Duarte, Andriele Wairich, Guilherme Paim Fraga, Marcio Pacheco Ribeiro, Helen Estima Lazzari
Summary: This study evaluates changes in phyllochron and thermal sum required for flowering in oat genotypes developed at different decades under different temperature regimes. The results show that the UFRGS 078030-2 genotype flowers earlier, has fewer developed phytomeres, and greater tolerance to high temperatures. More recent genotypes transition to reproductive stage earlier than older ones.
Article
Agronomy
Andrew R. S. de Queiroz, Carla A. Delatorre, Catarine Markus, Felipe R. Lucio, Paula S. Angonese, Aldo Merotto
Summary: Plants of Sumatran fleabane showed unusual rapid necrosis symptoms after application of herbicide 2,4-D. The resistance factor was high and the resistance may be related to reduced translocation, ABC transporters, auxin perception genes, or genes involved in response to pathogens and abiotic stresses. Inhibitors of detoxifying enzymes and transporters did not reduce the frequency of the rapid necrosis phenotype. The expression of certain genes decreased while others increased in response to 2,4-D.
Article
Agronomy
Paula Sinigaglia Angonese, Andrew Rerison Silva de Queiroz, Liana Sinigaglia Angonese, Filipi Mesquita Machado, Richard Napier, Catarine Markus, Carla A. Delatorre, Aldo Merotto Jr
Summary: Previous studies have characterized the resistant plants of Sumatran fleabane with rapid necrosis symptoms after applying 2,4-D. This study aimed to investigate the causes of variability in the occurrence of the rapid necrosis and found that environmental conditions, plant growth stage, and herbicide interactions can interfere with the symptoms. The herbicides dicamba and triclopyr effectively controlled resistant plants, especially when sprayed at the initial growth stages, providing insights into the mechanisms and causes of herbicide symptomology and performance variability.
Article
Plant Sciences
Diana Zabala-Pardo, Todd Gaines, Fabiane P. Lamego, Luis A. Avila
Summary: RNA interference (RNAi) is a promising technology for weed management, which involves spraying small RNAs (sRNAs) to induce gene silencing without the use of transgenes. However, the development of this technology in weed science has been slower compared to other crop protection areas due to the challenges in synthesizing stable sRNAs and ensuring their effectiveness. Once these challenges are overcome, RNAi technology could become a valuable tool for weed management and herbicide resistance management.
ADVANCES IN WEED SCIENCE
(2022)
Article
Agronomy
Eduardo Avelino Faleiro, Fabiane Pinto Lamego, Carlos Eduardo Schaedler, Tiago Antonio Del Valle, Eduardo Bohrer de Azevedo
Summary: This study evaluated the efficiency of different methods, including mechanical, physical, chemical, and cultural approaches, for controlling tough lovegrass. The results showed that isolated control methods were not effective, except for the use of glyphosate salts, which could control tough lovegrass plants. The best strategy for controlling the invasive plant was the combination of glyphosate salts, improved soil fertility, and the implantation of Pangola grass.
Article
Agriculture, Multidisciplinary
Dalvane Rockenbach, Andrisa Balbinot, Marlon Ouriques Bastiani, Fernanda Cassiane Caratti, Dirceu Agostinetto, Fabiane Lamego
Summary: This study evaluated the relative competition between white clover and weed species Silene gallica through replacement series, showing that the competitive relationship changes with different plant proportions. White clover displayed phenotypic plasticity, proving similar competitive abilities with Silene gallica.
Article
Agronomy
Tiago Edu Kaspary, Fabiane Pinto Lamego, Cristiano Belle, Adalin Cezar Moraes de Aguiar, Luan Cutti
Summary: The study assessed the responses of glyphosate-susceptible and -resistant hairy fleabane at different developmental stages and evaluated the physiological potential of seeds. Results showed that alternative herbicides effectively controlled resistant biotype at early stages, and resistant seeds exhibited stronger physiological potential.
Article
Agronomy
Maike Lovatto, Gerarda Beatriz Pinto da Silva, Filipe Kalikoski Coelho, Jose Antonio Martinelli, Marcelo Teixeira Pacheco, Luiz Carlos Federizzi, Carla Andrea Delatorre
Summary: This study aimed to characterize the crown rust resistance of oat genotypes and evaluate its impact on productivity and economy. Results showed that some oat genotypes exhibit high resistance to crown rust, while others are highly susceptible, with significant grain yield reduction. Genetic resistance, particularly partial resistance, plays a crucial role in maintaining oat yield potential, reducing environmental contamination, and increasing economic gains.
Article
Agronomy
Eduardo Avelino Faleiro, Diego Martins Chiapinotto, Fabiane Pinto Lamego, Carlos Eduardo Schaedler, Eduardo Bohrer de Azevedo
Summary: The study aimed to evaluate the efficiency of herbicides in controlling tough lovegrass under different environmental conditions and herbicides. Glyphosate was found to be an effective herbicide for tough lovegrass control under various resource conditions, while reductions in solar radiation improved the control effect of imazethapyr and cyhalofop. Flooded conditions did not significantly impact the control of tough lovegrass by herbicides.
Correction
Agronomy
Gerarda Beatriz Pinto da Silva, Camila Martini Zanella, Carla Andrea Delatorre, Marcia Soares Chaves, Jose Antonio Martinelli, Luiz Carlos Federizzi
Article
Agronomy
Tiago Edu Kaspary, Luan Cutti, Rafael Schwalm Rafaeli, Carla Andrea Delatorre, Aldo Merotto